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Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response

Porcine NK-Lysine (PNKL) is a new antimicrobial peptide (AMP) identified in the small intestine. In this study, PNKL protein was obtained through heterologous expression in Escherichia coli and was estimated by SDS-PAGE at 33 kDa. The antibacterial activities of PNKL were determined using various ba...

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Autores principales: Lin, Qian, Fu, Qingqing, Chen, Daiwen, Yu, Bing, Luo, Yuheng, Huang, Zhiqing, Zheng, Ping, Mao, Xiangbing, Yu, Jie, Luo, Junqiu, Yan, Hui, He, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307250/
https://www.ncbi.nlm.nih.gov/pubmed/34299517
http://dx.doi.org/10.3390/molecules26144242
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author Lin, Qian
Fu, Qingqing
Chen, Daiwen
Yu, Bing
Luo, Yuheng
Huang, Zhiqing
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
author_facet Lin, Qian
Fu, Qingqing
Chen, Daiwen
Yu, Bing
Luo, Yuheng
Huang, Zhiqing
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
author_sort Lin, Qian
collection PubMed
description Porcine NK-Lysine (PNKL) is a new antimicrobial peptide (AMP) identified in the small intestine. In this study, PNKL protein was obtained through heterologous expression in Escherichia coli and was estimated by SDS-PAGE at 33 kDa. The antibacterial activities of PNKL were determined using various bacterial strains and showed broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria. Furthermore, E. coli K88-challenged IPEC-J2 cells were used to determine PNKL influences on inflammatory responses. Hemolytic assays showed that PNKL had no detrimental impact on cell viability. Interestingly, PNKL elevated the viability of IPEC-J2 cells exposure to E. coli K88. PNKL significantly decreased the cell apoptosis rate, and improved the distribution and abundance of tight junction protein ZO-1 in IPEC-J2 cells upon E. coli K88-challenge. Importantly, PNKL not only down regulated the expressions of inflammatory cytokines such as the IL-6 and TNF-α, but also down regulated the expressions of NF-κB, Caspase3, and Caspase9 in the E. coli K88-challenged cells. These results suggest a novel function of natural killer (NK)-lysin, and the anti-bacterial and anti-inflammatory properties of PNKL may allow it a potential substitute for conventionally used antibiotics or drugs.
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spelling pubmed-83072502021-07-25 Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response Lin, Qian Fu, Qingqing Chen, Daiwen Yu, Bing Luo, Yuheng Huang, Zhiqing Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui He, Jun Molecules Article Porcine NK-Lysine (PNKL) is a new antimicrobial peptide (AMP) identified in the small intestine. In this study, PNKL protein was obtained through heterologous expression in Escherichia coli and was estimated by SDS-PAGE at 33 kDa. The antibacterial activities of PNKL were determined using various bacterial strains and showed broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria. Furthermore, E. coli K88-challenged IPEC-J2 cells were used to determine PNKL influences on inflammatory responses. Hemolytic assays showed that PNKL had no detrimental impact on cell viability. Interestingly, PNKL elevated the viability of IPEC-J2 cells exposure to E. coli K88. PNKL significantly decreased the cell apoptosis rate, and improved the distribution and abundance of tight junction protein ZO-1 in IPEC-J2 cells upon E. coli K88-challenge. Importantly, PNKL not only down regulated the expressions of inflammatory cytokines such as the IL-6 and TNF-α, but also down regulated the expressions of NF-κB, Caspase3, and Caspase9 in the E. coli K88-challenged cells. These results suggest a novel function of natural killer (NK)-lysin, and the anti-bacterial and anti-inflammatory properties of PNKL may allow it a potential substitute for conventionally used antibiotics or drugs. MDPI 2021-07-13 /pmc/articles/PMC8307250/ /pubmed/34299517 http://dx.doi.org/10.3390/molecules26144242 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Qian
Fu, Qingqing
Chen, Daiwen
Yu, Bing
Luo, Yuheng
Huang, Zhiqing
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response
title Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response
title_full Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response
title_fullStr Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response
title_full_unstemmed Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response
title_short Functional Characterization of Porcine NK-Lysin: A Novel Immunomodulator That Regulates Intestinal Inflammatory Response
title_sort functional characterization of porcine nk-lysin: a novel immunomodulator that regulates intestinal inflammatory response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307250/
https://www.ncbi.nlm.nih.gov/pubmed/34299517
http://dx.doi.org/10.3390/molecules26144242
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