Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe

Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a nov...

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Autores principales: Panfilov, Mikhail, Chernova, Darya, Khalfina, Irina, Moskalensky, Alexander, Vorob’ev, Aleksey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307419/
https://www.ncbi.nlm.nih.gov/pubmed/34299615
http://dx.doi.org/10.3390/molecules26144340
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author Panfilov, Mikhail
Chernova, Darya
Khalfina, Irina
Moskalensky, Alexander
Vorob’ev, Aleksey
author_facet Panfilov, Mikhail
Chernova, Darya
Khalfina, Irina
Moskalensky, Alexander
Vorob’ev, Aleksey
author_sort Panfilov, Mikhail
collection PubMed
description Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10H)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10H)acridone reacts with NO in aqueous media in the presence of O(2), yielding a corresponding triazole derivative with fivefold increased fluorescence intensity. The probe was shown to be capable of nitric oxide sensing in living Jurkat cells.
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spelling pubmed-83074192021-07-25 Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe Panfilov, Mikhail Chernova, Darya Khalfina, Irina Moskalensky, Alexander Vorob’ev, Aleksey Molecules Article Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10H)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10H)acridone reacts with NO in aqueous media in the presence of O(2), yielding a corresponding triazole derivative with fivefold increased fluorescence intensity. The probe was shown to be capable of nitric oxide sensing in living Jurkat cells. MDPI 2021-07-17 /pmc/articles/PMC8307419/ /pubmed/34299615 http://dx.doi.org/10.3390/molecules26144340 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panfilov, Mikhail
Chernova, Darya
Khalfina, Irina
Moskalensky, Alexander
Vorob’ev, Aleksey
Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
title Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
title_full Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
title_fullStr Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
title_full_unstemmed Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
title_short Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
title_sort design and synthesis of new acridone-based nitric oxide fluorescent probe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307419/
https://www.ncbi.nlm.nih.gov/pubmed/34299615
http://dx.doi.org/10.3390/molecules26144340
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AT moskalenskyalexander designandsynthesisofnewacridonebasednitricoxidefluorescentprobe
AT vorobevaleksey designandsynthesisofnewacridonebasednitricoxidefluorescentprobe

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