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Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging

The aim of this study was to develop a bioimaging probe based on magnetic iron oxide nanoparticles (MIONs) surface functionalized with the copolymer (p(MAA-g-EGMA)), which were radiolabeled with the positron emitter Gallium-68. The synthesis of the hybrid MIONs was realized by hydrolytic condensatio...

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Autores principales: Papadopoulou, Sofia, Kolokithas-Ntoukas, Argiris, Salvanou, Evangelia-Alexandra, Gaitanis, Anastasios, Xanthopoulos, Stavros, Avgoustakis, Konstantinos, Gazouli, Maria, Paravatou-Petsotas, Maria, Tsoukalas, Charalampos, Bakandritsos, Aristides, Bouziotis, Penelope
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307582/
https://www.ncbi.nlm.nih.gov/pubmed/34202370
http://dx.doi.org/10.3390/nano11071677
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author Papadopoulou, Sofia
Kolokithas-Ntoukas, Argiris
Salvanou, Evangelia-Alexandra
Gaitanis, Anastasios
Xanthopoulos, Stavros
Avgoustakis, Konstantinos
Gazouli, Maria
Paravatou-Petsotas, Maria
Tsoukalas, Charalampos
Bakandritsos, Aristides
Bouziotis, Penelope
author_facet Papadopoulou, Sofia
Kolokithas-Ntoukas, Argiris
Salvanou, Evangelia-Alexandra
Gaitanis, Anastasios
Xanthopoulos, Stavros
Avgoustakis, Konstantinos
Gazouli, Maria
Paravatou-Petsotas, Maria
Tsoukalas, Charalampos
Bakandritsos, Aristides
Bouziotis, Penelope
author_sort Papadopoulou, Sofia
collection PubMed
description The aim of this study was to develop a bioimaging probe based on magnetic iron oxide nanoparticles (MIONs) surface functionalized with the copolymer (p(MAA-g-EGMA)), which were radiolabeled with the positron emitter Gallium-68. The synthesis of the hybrid MIONs was realized by hydrolytic condensation of a single ferrous precursor in the presence of the copolymer. The synthesized MagP MIONs displayed an average D(h) of 87 nm, suitable for passive targeting of cancerous tissues through the enhanced permeation and retention (EPR) effect after intravenous administration, while their particularly high magnetic content ascribes strong magnetic properties to the colloids. Two different approaches were explored to develop MIONs radiolabeled with (68)Ga: the chelator-mediated approach, where the chelating agent NODAGA-NHS was conjugated onto the MIONs (MagP-NODAGA) to form a chelate complex with (68)Ga, and the chelator-free approach, where (68)Ga was directly incorporated onto the MIONs (MagP). Both groups of NPs showed highly efficient radiolabeling with (68)Ga, forming constructs which were stable with time, and in the presence of PBS and human serum. Ex vivo biodistribution studies of [(68)Ga]Ga- MIONs showed high accumulation in the mononuclear phagocyte system (MPS) organs and satisfactory blood retention with time. In vivo PET imaging with [(68)Ga]Ga-MagP MIONs was in accordance with the ex vivo biodistribution results. Finally, the MIONs showed low toxicity against 4T1 breast cancer cells. These detailed studies established that [(68)Ga]Ga- MIONs exhibit potential for application as tracers for early cancer detection.
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spelling pubmed-83075822021-07-25 Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging Papadopoulou, Sofia Kolokithas-Ntoukas, Argiris Salvanou, Evangelia-Alexandra Gaitanis, Anastasios Xanthopoulos, Stavros Avgoustakis, Konstantinos Gazouli, Maria Paravatou-Petsotas, Maria Tsoukalas, Charalampos Bakandritsos, Aristides Bouziotis, Penelope Nanomaterials (Basel) Article The aim of this study was to develop a bioimaging probe based on magnetic iron oxide nanoparticles (MIONs) surface functionalized with the copolymer (p(MAA-g-EGMA)), which were radiolabeled with the positron emitter Gallium-68. The synthesis of the hybrid MIONs was realized by hydrolytic condensation of a single ferrous precursor in the presence of the copolymer. The synthesized MagP MIONs displayed an average D(h) of 87 nm, suitable for passive targeting of cancerous tissues through the enhanced permeation and retention (EPR) effect after intravenous administration, while their particularly high magnetic content ascribes strong magnetic properties to the colloids. Two different approaches were explored to develop MIONs radiolabeled with (68)Ga: the chelator-mediated approach, where the chelating agent NODAGA-NHS was conjugated onto the MIONs (MagP-NODAGA) to form a chelate complex with (68)Ga, and the chelator-free approach, where (68)Ga was directly incorporated onto the MIONs (MagP). Both groups of NPs showed highly efficient radiolabeling with (68)Ga, forming constructs which were stable with time, and in the presence of PBS and human serum. Ex vivo biodistribution studies of [(68)Ga]Ga- MIONs showed high accumulation in the mononuclear phagocyte system (MPS) organs and satisfactory blood retention with time. In vivo PET imaging with [(68)Ga]Ga-MagP MIONs was in accordance with the ex vivo biodistribution results. Finally, the MIONs showed low toxicity against 4T1 breast cancer cells. These detailed studies established that [(68)Ga]Ga- MIONs exhibit potential for application as tracers for early cancer detection. MDPI 2021-06-25 /pmc/articles/PMC8307582/ /pubmed/34202370 http://dx.doi.org/10.3390/nano11071677 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Papadopoulou, Sofia
Kolokithas-Ntoukas, Argiris
Salvanou, Evangelia-Alexandra
Gaitanis, Anastasios
Xanthopoulos, Stavros
Avgoustakis, Konstantinos
Gazouli, Maria
Paravatou-Petsotas, Maria
Tsoukalas, Charalampos
Bakandritsos, Aristides
Bouziotis, Penelope
Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
title Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
title_full Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
title_fullStr Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
title_full_unstemmed Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
title_short Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
title_sort chelator-free/chelator-mediated radiolabeling of colloidally stabilized iron oxide nanoparticles for biomedical imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307582/
https://www.ncbi.nlm.nih.gov/pubmed/34202370
http://dx.doi.org/10.3390/nano11071677
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