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Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging
The aim of this study was to develop a bioimaging probe based on magnetic iron oxide nanoparticles (MIONs) surface functionalized with the copolymer (p(MAA-g-EGMA)), which were radiolabeled with the positron emitter Gallium-68. The synthesis of the hybrid MIONs was realized by hydrolytic condensatio...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307582/ https://www.ncbi.nlm.nih.gov/pubmed/34202370 http://dx.doi.org/10.3390/nano11071677 |
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author | Papadopoulou, Sofia Kolokithas-Ntoukas, Argiris Salvanou, Evangelia-Alexandra Gaitanis, Anastasios Xanthopoulos, Stavros Avgoustakis, Konstantinos Gazouli, Maria Paravatou-Petsotas, Maria Tsoukalas, Charalampos Bakandritsos, Aristides Bouziotis, Penelope |
author_facet | Papadopoulou, Sofia Kolokithas-Ntoukas, Argiris Salvanou, Evangelia-Alexandra Gaitanis, Anastasios Xanthopoulos, Stavros Avgoustakis, Konstantinos Gazouli, Maria Paravatou-Petsotas, Maria Tsoukalas, Charalampos Bakandritsos, Aristides Bouziotis, Penelope |
author_sort | Papadopoulou, Sofia |
collection | PubMed |
description | The aim of this study was to develop a bioimaging probe based on magnetic iron oxide nanoparticles (MIONs) surface functionalized with the copolymer (p(MAA-g-EGMA)), which were radiolabeled with the positron emitter Gallium-68. The synthesis of the hybrid MIONs was realized by hydrolytic condensation of a single ferrous precursor in the presence of the copolymer. The synthesized MagP MIONs displayed an average D(h) of 87 nm, suitable for passive targeting of cancerous tissues through the enhanced permeation and retention (EPR) effect after intravenous administration, while their particularly high magnetic content ascribes strong magnetic properties to the colloids. Two different approaches were explored to develop MIONs radiolabeled with (68)Ga: the chelator-mediated approach, where the chelating agent NODAGA-NHS was conjugated onto the MIONs (MagP-NODAGA) to form a chelate complex with (68)Ga, and the chelator-free approach, where (68)Ga was directly incorporated onto the MIONs (MagP). Both groups of NPs showed highly efficient radiolabeling with (68)Ga, forming constructs which were stable with time, and in the presence of PBS and human serum. Ex vivo biodistribution studies of [(68)Ga]Ga- MIONs showed high accumulation in the mononuclear phagocyte system (MPS) organs and satisfactory blood retention with time. In vivo PET imaging with [(68)Ga]Ga-MagP MIONs was in accordance with the ex vivo biodistribution results. Finally, the MIONs showed low toxicity against 4T1 breast cancer cells. These detailed studies established that [(68)Ga]Ga- MIONs exhibit potential for application as tracers for early cancer detection. |
format | Online Article Text |
id | pubmed-8307582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83075822021-07-25 Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging Papadopoulou, Sofia Kolokithas-Ntoukas, Argiris Salvanou, Evangelia-Alexandra Gaitanis, Anastasios Xanthopoulos, Stavros Avgoustakis, Konstantinos Gazouli, Maria Paravatou-Petsotas, Maria Tsoukalas, Charalampos Bakandritsos, Aristides Bouziotis, Penelope Nanomaterials (Basel) Article The aim of this study was to develop a bioimaging probe based on magnetic iron oxide nanoparticles (MIONs) surface functionalized with the copolymer (p(MAA-g-EGMA)), which were radiolabeled with the positron emitter Gallium-68. The synthesis of the hybrid MIONs was realized by hydrolytic condensation of a single ferrous precursor in the presence of the copolymer. The synthesized MagP MIONs displayed an average D(h) of 87 nm, suitable for passive targeting of cancerous tissues through the enhanced permeation and retention (EPR) effect after intravenous administration, while their particularly high magnetic content ascribes strong magnetic properties to the colloids. Two different approaches were explored to develop MIONs radiolabeled with (68)Ga: the chelator-mediated approach, where the chelating agent NODAGA-NHS was conjugated onto the MIONs (MagP-NODAGA) to form a chelate complex with (68)Ga, and the chelator-free approach, where (68)Ga was directly incorporated onto the MIONs (MagP). Both groups of NPs showed highly efficient radiolabeling with (68)Ga, forming constructs which were stable with time, and in the presence of PBS and human serum. Ex vivo biodistribution studies of [(68)Ga]Ga- MIONs showed high accumulation in the mononuclear phagocyte system (MPS) organs and satisfactory blood retention with time. In vivo PET imaging with [(68)Ga]Ga-MagP MIONs was in accordance with the ex vivo biodistribution results. Finally, the MIONs showed low toxicity against 4T1 breast cancer cells. These detailed studies established that [(68)Ga]Ga- MIONs exhibit potential for application as tracers for early cancer detection. MDPI 2021-06-25 /pmc/articles/PMC8307582/ /pubmed/34202370 http://dx.doi.org/10.3390/nano11071677 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Papadopoulou, Sofia Kolokithas-Ntoukas, Argiris Salvanou, Evangelia-Alexandra Gaitanis, Anastasios Xanthopoulos, Stavros Avgoustakis, Konstantinos Gazouli, Maria Paravatou-Petsotas, Maria Tsoukalas, Charalampos Bakandritsos, Aristides Bouziotis, Penelope Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging |
title | Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging |
title_full | Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging |
title_fullStr | Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging |
title_full_unstemmed | Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging |
title_short | Chelator-Free/Chelator-Mediated Radiolabeling of Colloidally Stabilized Iron Oxide Nanoparticles for Biomedical Imaging |
title_sort | chelator-free/chelator-mediated radiolabeling of colloidally stabilized iron oxide nanoparticles for biomedical imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307582/ https://www.ncbi.nlm.nih.gov/pubmed/34202370 http://dx.doi.org/10.3390/nano11071677 |
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