Combined FCS and PCH Analysis to Quantify Protein Dimerization in Living Cells

Protein dimerization plays a crucial role in the regulation of numerous biological processes. However, detecting protein dimers in a cellular environment is still a challenge. Here we present a methodology to measure the extent of dimerization of GFP-tagged proteins in living cells, using a combinat...

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Detalles Bibliográficos
Autores principales: Nederveen-Schippers, Laura M., Pathak, Pragya, Keizer-Gunnink, Ineke, Westphal, Adrie H., van Haastert, Peter J. M., Borst, Jan Willem, Kortholt, Arjan, Skakun, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307594/
https://www.ncbi.nlm.nih.gov/pubmed/34298920
http://dx.doi.org/10.3390/ijms22147300

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307594/
https://www.ncbi.nlm.nih.gov/pubmed/34298920
http://dx.doi.org/10.3390/ijms22147300

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