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Combined FCS and PCH Analysis to Quantify Protein Dimerization in Living Cells
Protein dimerization plays a crucial role in the regulation of numerous biological processes. However, detecting protein dimers in a cellular environment is still a challenge. Here we present a methodology to measure the extent of dimerization of GFP-tagged proteins in living cells, using a combinat...
Autores principales: | Nederveen-Schippers, Laura M., Pathak, Pragya, Keizer-Gunnink, Ineke, Westphal, Adrie H., van Haastert, Peter J. M., Borst, Jan Willem, Kortholt, Arjan, Skakun, Victor |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307594/ https://www.ncbi.nlm.nih.gov/pubmed/34298920 http://dx.doi.org/10.3390/ijms22147300 |
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