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Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer
MicroRNA and DNA adduct biomarkers may be used to identify the contribution of environmental pollution to some types of cancers. The aim of this study was to use integrated DNA adducts and microRNAs analyses to study retrospectively the contribution of exposures to environmental carcinogens to lung...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307636/ https://www.ncbi.nlm.nih.gov/pubmed/34357133 http://dx.doi.org/10.3390/jpm11070666 |
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author | Izzotti, Alberto Coronel Vargas, Gabriela Pulliero, Alessandra Coco, Simona Colarossi, Cristina Blanco, Giuseppina Agodi, Antonella Barchitta, Martina Maugeri, Andrea Oliveri Conti, Gea Ferrante, Margherita Sciacca, Salvatore |
author_facet | Izzotti, Alberto Coronel Vargas, Gabriela Pulliero, Alessandra Coco, Simona Colarossi, Cristina Blanco, Giuseppina Agodi, Antonella Barchitta, Martina Maugeri, Andrea Oliveri Conti, Gea Ferrante, Margherita Sciacca, Salvatore |
author_sort | Izzotti, Alberto |
collection | PubMed |
description | MicroRNA and DNA adduct biomarkers may be used to identify the contribution of environmental pollution to some types of cancers. The aim of this study was to use integrated DNA adducts and microRNAs analyses to study retrospectively the contribution of exposures to environmental carcinogens to lung cancer in 64 non-smokers living in Sicily and Catania city near to the Etna volcano. MicroRNAs were extracted from cancer lung biopsies, and from the surrounding lung normal tissue. The expression of 2549 human microRNAs was analyzed by microarray. Benzo(a)Pyrene-DNA adducts levels were analyzed in the patients’ blood by HPLC−fluorescence detection. Correlations between tetrols and environmental exposures were calculated using Pearson coefficients and regression variable plots. Compared with the healthy tissue, 273 microRNAs were downregulated in lung cancer. Tetrols levels were inversely related both with the distance from Etna and years since smoking cessation, but they were not significantly correlated to environmental exposures. The analysis of the microRNA environmental signatures indicates the contribution of environmental factors to the analyzed lung cancers in the following decreasing rank: (a) car traffic, (b) passive smoke, (c) radon, and (d) volcano ashes. These results provide evidence that microRNA analysis can be used to retrospectively investigate the contribution of environmental factors in human lung cancer occurring in non-smokers. |
format | Online Article Text |
id | pubmed-8307636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83076362021-07-25 Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer Izzotti, Alberto Coronel Vargas, Gabriela Pulliero, Alessandra Coco, Simona Colarossi, Cristina Blanco, Giuseppina Agodi, Antonella Barchitta, Martina Maugeri, Andrea Oliveri Conti, Gea Ferrante, Margherita Sciacca, Salvatore J Pers Med Article MicroRNA and DNA adduct biomarkers may be used to identify the contribution of environmental pollution to some types of cancers. The aim of this study was to use integrated DNA adducts and microRNAs analyses to study retrospectively the contribution of exposures to environmental carcinogens to lung cancer in 64 non-smokers living in Sicily and Catania city near to the Etna volcano. MicroRNAs were extracted from cancer lung biopsies, and from the surrounding lung normal tissue. The expression of 2549 human microRNAs was analyzed by microarray. Benzo(a)Pyrene-DNA adducts levels were analyzed in the patients’ blood by HPLC−fluorescence detection. Correlations between tetrols and environmental exposures were calculated using Pearson coefficients and regression variable plots. Compared with the healthy tissue, 273 microRNAs were downregulated in lung cancer. Tetrols levels were inversely related both with the distance from Etna and years since smoking cessation, but they were not significantly correlated to environmental exposures. The analysis of the microRNA environmental signatures indicates the contribution of environmental factors to the analyzed lung cancers in the following decreasing rank: (a) car traffic, (b) passive smoke, (c) radon, and (d) volcano ashes. These results provide evidence that microRNA analysis can be used to retrospectively investigate the contribution of environmental factors in human lung cancer occurring in non-smokers. MDPI 2021-07-15 /pmc/articles/PMC8307636/ /pubmed/34357133 http://dx.doi.org/10.3390/jpm11070666 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Izzotti, Alberto Coronel Vargas, Gabriela Pulliero, Alessandra Coco, Simona Colarossi, Cristina Blanco, Giuseppina Agodi, Antonella Barchitta, Martina Maugeri, Andrea Oliveri Conti, Gea Ferrante, Margherita Sciacca, Salvatore Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer |
title | Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer |
title_full | Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer |
title_fullStr | Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer |
title_full_unstemmed | Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer |
title_short | Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer |
title_sort | identification by microrna analysis of environmental risk factors bearing pathogenic relevance in non-smoker lung cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307636/ https://www.ncbi.nlm.nih.gov/pubmed/34357133 http://dx.doi.org/10.3390/jpm11070666 |
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