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N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study
The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephri...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307704/ https://www.ncbi.nlm.nih.gov/pubmed/34299175 http://dx.doi.org/10.3390/ijms22147549 |
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author | Paskal, Wiktor Paskal, Adriana M. Pietruski, Piotr Stachura, Albert Pełka, Kacper Woessner, Alan E. Quinn, Kyle P. Kopka, Michał Galus, Ryszard Wejman, Jarosław Włodarski, Paweł |
author_facet | Paskal, Wiktor Paskal, Adriana M. Pietruski, Piotr Stachura, Albert Pełka, Kacper Woessner, Alan E. Quinn, Kyle P. Kopka, Michał Galus, Ryszard Wejman, Jarosław Włodarski, Paweł |
author_sort | Paskal, Wiktor |
collection | PubMed |
description | The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration. |
format | Online Article Text |
id | pubmed-8307704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83077042021-07-25 N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study Paskal, Wiktor Paskal, Adriana M. Pietruski, Piotr Stachura, Albert Pełka, Kacper Woessner, Alan E. Quinn, Kyle P. Kopka, Michał Galus, Ryszard Wejman, Jarosław Włodarski, Paweł Int J Mol Sci Article The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration. MDPI 2021-07-14 /pmc/articles/PMC8307704/ /pubmed/34299175 http://dx.doi.org/10.3390/ijms22147549 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Paskal, Wiktor Paskal, Adriana M. Pietruski, Piotr Stachura, Albert Pełka, Kacper Woessner, Alan E. Quinn, Kyle P. Kopka, Michał Galus, Ryszard Wejman, Jarosław Włodarski, Paweł N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study |
title | N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study |
title_full | N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study |
title_fullStr | N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study |
title_full_unstemmed | N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study |
title_short | N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study |
title_sort | n-acetylcysteine added to local anesthesia reduces scar area and width in early wound healing—an animal model study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307704/ https://www.ncbi.nlm.nih.gov/pubmed/34299175 http://dx.doi.org/10.3390/ijms22147549 |
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