Cargando…
Identification of Cilia in Different Mouse Tissues
Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary l...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307782/ https://www.ncbi.nlm.nih.gov/pubmed/34209603 http://dx.doi.org/10.3390/cells10071623 |
_version_ | 1783728127998951424 |
---|---|
author | Li, Xinhua Yang, Shuting Deepk, Vishwa Chinipardaz, Zahra Yang, Shuying |
author_facet | Li, Xinhua Yang, Shuting Deepk, Vishwa Chinipardaz, Zahra Yang, Shuying |
author_sort | Li, Xinhua |
collection | PubMed |
description | Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary lengths and the percentage of ciliated cells in different organs and tissues in vivo by using cilium dual reporter-expressing transgenic mice. Cilia were identified by the presence of ARL13B with an mCherry+ signal, and the cilium basal body was identified by the presence of Centrin2 with a GFP+ signal. Here, we provide in vivo evidence that chondrocytes and cells throughout bones have cilia. Most importantly, we reveal that: 1. primary cilia are present in hepatocytes; 2. no cilia but many centrioles are distributed on the apical cell surface in the gallbladder, intestine, and thyroid epithelia; 3. cilia on the cerebral cortex are well oriented, pointing to the center of the brain; 4. ARL13B+ inclusion is evident in the thyroid and islets of Langerhans; and 5. approximately 2% of cilia show irregular movement in nucleus pulposus extracellular fluid. This study reveals the existence and distribution of cilia and centrioles in different tissues and organs, and provides new insights for further comprehensive study of ciliary function in these organs and tissues. |
format | Online Article Text |
id | pubmed-8307782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83077822021-07-25 Identification of Cilia in Different Mouse Tissues Li, Xinhua Yang, Shuting Deepk, Vishwa Chinipardaz, Zahra Yang, Shuying Cells Article Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary lengths and the percentage of ciliated cells in different organs and tissues in vivo by using cilium dual reporter-expressing transgenic mice. Cilia were identified by the presence of ARL13B with an mCherry+ signal, and the cilium basal body was identified by the presence of Centrin2 with a GFP+ signal. Here, we provide in vivo evidence that chondrocytes and cells throughout bones have cilia. Most importantly, we reveal that: 1. primary cilia are present in hepatocytes; 2. no cilia but many centrioles are distributed on the apical cell surface in the gallbladder, intestine, and thyroid epithelia; 3. cilia on the cerebral cortex are well oriented, pointing to the center of the brain; 4. ARL13B+ inclusion is evident in the thyroid and islets of Langerhans; and 5. approximately 2% of cilia show irregular movement in nucleus pulposus extracellular fluid. This study reveals the existence and distribution of cilia and centrioles in different tissues and organs, and provides new insights for further comprehensive study of ciliary function in these organs and tissues. MDPI 2021-06-29 /pmc/articles/PMC8307782/ /pubmed/34209603 http://dx.doi.org/10.3390/cells10071623 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Xinhua Yang, Shuting Deepk, Vishwa Chinipardaz, Zahra Yang, Shuying Identification of Cilia in Different Mouse Tissues |
title | Identification of Cilia in Different Mouse Tissues |
title_full | Identification of Cilia in Different Mouse Tissues |
title_fullStr | Identification of Cilia in Different Mouse Tissues |
title_full_unstemmed | Identification of Cilia in Different Mouse Tissues |
title_short | Identification of Cilia in Different Mouse Tissues |
title_sort | identification of cilia in different mouse tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307782/ https://www.ncbi.nlm.nih.gov/pubmed/34209603 http://dx.doi.org/10.3390/cells10071623 |
work_keys_str_mv | AT lixinhua identificationofciliaindifferentmousetissues AT yangshuting identificationofciliaindifferentmousetissues AT deepkvishwa identificationofciliaindifferentmousetissues AT chinipardazzahra identificationofciliaindifferentmousetissues AT yangshuying identificationofciliaindifferentmousetissues |