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Identification of Cilia in Different Mouse Tissues

Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary l...

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Autores principales: Li, Xinhua, Yang, Shuting, Deepk, Vishwa, Chinipardaz, Zahra, Yang, Shuying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307782/
https://www.ncbi.nlm.nih.gov/pubmed/34209603
http://dx.doi.org/10.3390/cells10071623
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author Li, Xinhua
Yang, Shuting
Deepk, Vishwa
Chinipardaz, Zahra
Yang, Shuying
author_facet Li, Xinhua
Yang, Shuting
Deepk, Vishwa
Chinipardaz, Zahra
Yang, Shuying
author_sort Li, Xinhua
collection PubMed
description Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary lengths and the percentage of ciliated cells in different organs and tissues in vivo by using cilium dual reporter-expressing transgenic mice. Cilia were identified by the presence of ARL13B with an mCherry+ signal, and the cilium basal body was identified by the presence of Centrin2 with a GFP+ signal. Here, we provide in vivo evidence that chondrocytes and cells throughout bones have cilia. Most importantly, we reveal that: 1. primary cilia are present in hepatocytes; 2. no cilia but many centrioles are distributed on the apical cell surface in the gallbladder, intestine, and thyroid epithelia; 3. cilia on the cerebral cortex are well oriented, pointing to the center of the brain; 4. ARL13B+ inclusion is evident in the thyroid and islets of Langerhans; and 5. approximately 2% of cilia show irregular movement in nucleus pulposus extracellular fluid. This study reveals the existence and distribution of cilia and centrioles in different tissues and organs, and provides new insights for further comprehensive study of ciliary function in these organs and tissues.
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spelling pubmed-83077822021-07-25 Identification of Cilia in Different Mouse Tissues Li, Xinhua Yang, Shuting Deepk, Vishwa Chinipardaz, Zahra Yang, Shuying Cells Article Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary lengths and the percentage of ciliated cells in different organs and tissues in vivo by using cilium dual reporter-expressing transgenic mice. Cilia were identified by the presence of ARL13B with an mCherry+ signal, and the cilium basal body was identified by the presence of Centrin2 with a GFP+ signal. Here, we provide in vivo evidence that chondrocytes and cells throughout bones have cilia. Most importantly, we reveal that: 1. primary cilia are present in hepatocytes; 2. no cilia but many centrioles are distributed on the apical cell surface in the gallbladder, intestine, and thyroid epithelia; 3. cilia on the cerebral cortex are well oriented, pointing to the center of the brain; 4. ARL13B+ inclusion is evident in the thyroid and islets of Langerhans; and 5. approximately 2% of cilia show irregular movement in nucleus pulposus extracellular fluid. This study reveals the existence and distribution of cilia and centrioles in different tissues and organs, and provides new insights for further comprehensive study of ciliary function in these organs and tissues. MDPI 2021-06-29 /pmc/articles/PMC8307782/ /pubmed/34209603 http://dx.doi.org/10.3390/cells10071623 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xinhua
Yang, Shuting
Deepk, Vishwa
Chinipardaz, Zahra
Yang, Shuying
Identification of Cilia in Different Mouse Tissues
title Identification of Cilia in Different Mouse Tissues
title_full Identification of Cilia in Different Mouse Tissues
title_fullStr Identification of Cilia in Different Mouse Tissues
title_full_unstemmed Identification of Cilia in Different Mouse Tissues
title_short Identification of Cilia in Different Mouse Tissues
title_sort identification of cilia in different mouse tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307782/
https://www.ncbi.nlm.nih.gov/pubmed/34209603
http://dx.doi.org/10.3390/cells10071623
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AT deepkvishwa identificationofciliaindifferentmousetissues
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AT yangshuying identificationofciliaindifferentmousetissues