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Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury

Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the...

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Autores principales: Rakib, Fazle, Al-Saad, Khalid, Ustaoglu, Sebnem Garip, Ullah, Ehsan, Mall, Raghvendra, Thompson, Richard, Abdelalim, Essam M., Ahmed, Tariq, Severcan, Feride, Ali, Mohamed H. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307811/
https://www.ncbi.nlm.nih.gov/pubmed/34356152
http://dx.doi.org/10.3390/brainsci11070918
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author Rakib, Fazle
Al-Saad, Khalid
Ustaoglu, Sebnem Garip
Ullah, Ehsan
Mall, Raghvendra
Thompson, Richard
Abdelalim, Essam M.
Ahmed, Tariq
Severcan, Feride
Ali, Mohamed H. M.
author_facet Rakib, Fazle
Al-Saad, Khalid
Ustaoglu, Sebnem Garip
Ullah, Ehsan
Mall, Raghvendra
Thompson, Richard
Abdelalim, Essam M.
Ahmed, Tariq
Severcan, Feride
Ali, Mohamed H. M.
author_sort Rakib, Fazle
collection PubMed
description Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.
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spelling pubmed-83078112021-07-25 Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury Rakib, Fazle Al-Saad, Khalid Ustaoglu, Sebnem Garip Ullah, Ehsan Mall, Raghvendra Thompson, Richard Abdelalim, Essam M. Ahmed, Tariq Severcan, Feride Ali, Mohamed H. M. Brain Sci Article Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution. MDPI 2021-07-12 /pmc/articles/PMC8307811/ /pubmed/34356152 http://dx.doi.org/10.3390/brainsci11070918 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rakib, Fazle
Al-Saad, Khalid
Ustaoglu, Sebnem Garip
Ullah, Ehsan
Mall, Raghvendra
Thompson, Richard
Abdelalim, Essam M.
Ahmed, Tariq
Severcan, Feride
Ali, Mohamed H. M.
Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
title Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
title_full Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
title_fullStr Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
title_full_unstemmed Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
title_short Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
title_sort fourier transform infrared imaging—a novel approach to monitor bio molecular changes in subacute mild traumatic brain injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307811/
https://www.ncbi.nlm.nih.gov/pubmed/34356152
http://dx.doi.org/10.3390/brainsci11070918
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