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Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.

Two new species, Penicillium krskae (isolated from the air as a lab contaminant in Tulln (Austria, EU)) and Penicillium silybi (isolated as an endophyte from asymptomatic milk thistle (Silybum marianum) stems from Josephine County (Oregon, USA)) are described. The new taxa are well supported by phen...

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Autores principales: Labuda, Roman, Bacher, Markus, Rosenau, Thomas, Gasparotto, Erika, Gratzl, Hannes, Doppler, Maria, Sulyok, Michael, Kubátová, Alena, Berger, Harald, Cank, Kristof, Raja, Huzefa A., Oberlies, Nicholas H., Schüller, Christoph, Strauss, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307998/
https://www.ncbi.nlm.nih.gov/pubmed/34356936
http://dx.doi.org/10.3390/jof7070557
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author Labuda, Roman
Bacher, Markus
Rosenau, Thomas
Gasparotto, Erika
Gratzl, Hannes
Doppler, Maria
Sulyok, Michael
Kubátová, Alena
Berger, Harald
Cank, Kristof
Raja, Huzefa A.
Oberlies, Nicholas H.
Schüller, Christoph
Strauss, Joseph
author_facet Labuda, Roman
Bacher, Markus
Rosenau, Thomas
Gasparotto, Erika
Gratzl, Hannes
Doppler, Maria
Sulyok, Michael
Kubátová, Alena
Berger, Harald
Cank, Kristof
Raja, Huzefa A.
Oberlies, Nicholas H.
Schüller, Christoph
Strauss, Joseph
author_sort Labuda, Roman
collection PubMed
description Two new species, Penicillium krskae (isolated from the air as a lab contaminant in Tulln (Austria, EU)) and Penicillium silybi (isolated as an endophyte from asymptomatic milk thistle (Silybum marianum) stems from Josephine County (Oregon, USA)) are described. The new taxa are well supported by phenotypic (especially conidial ornamentation under SEM, production of red exudate and red pigments), physiological (growth at 37 °C, response to cycloheximide and CREA), chemotaxonomic (production of specific extrolites), and multilocus phylogenetic analysis using RNA-polymerase II second largest subunit (RPB2), partial tubulin (benA), and calmodulin (CaM). Both new taxa are resolved within the section Exilicaulis in series Restricta and show phylogenetic affiliation to P. restrictum sensu stricto. They produce a large spectrum of toxic anthraquinoid pigments, namely, monomeric anthraquinones related to emodic and chloremodic acids and other interesting bioactive extrolites (i.e., endocrocin, paxilline, pestalotin, and 7-hydroxypestalotin). Of note, two bianthraquinones (i.e., skyrin and oxyskyrin) were detected in a culture extract of P. silybi. Two new chloroemodic acid derivatives (2-chloro-isorhodoptilometrin and 2-chloro-desmethyldermoquinone) isolated from the exudate of P. krskae ex-type culture were analyzed by nuclear magnetic resonance (NMR) and liquid chromatography–mass spectrometry (LC–MS).
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spelling pubmed-83079982021-07-25 Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov. Labuda, Roman Bacher, Markus Rosenau, Thomas Gasparotto, Erika Gratzl, Hannes Doppler, Maria Sulyok, Michael Kubátová, Alena Berger, Harald Cank, Kristof Raja, Huzefa A. Oberlies, Nicholas H. Schüller, Christoph Strauss, Joseph J Fungi (Basel) Article Two new species, Penicillium krskae (isolated from the air as a lab contaminant in Tulln (Austria, EU)) and Penicillium silybi (isolated as an endophyte from asymptomatic milk thistle (Silybum marianum) stems from Josephine County (Oregon, USA)) are described. The new taxa are well supported by phenotypic (especially conidial ornamentation under SEM, production of red exudate and red pigments), physiological (growth at 37 °C, response to cycloheximide and CREA), chemotaxonomic (production of specific extrolites), and multilocus phylogenetic analysis using RNA-polymerase II second largest subunit (RPB2), partial tubulin (benA), and calmodulin (CaM). Both new taxa are resolved within the section Exilicaulis in series Restricta and show phylogenetic affiliation to P. restrictum sensu stricto. They produce a large spectrum of toxic anthraquinoid pigments, namely, monomeric anthraquinones related to emodic and chloremodic acids and other interesting bioactive extrolites (i.e., endocrocin, paxilline, pestalotin, and 7-hydroxypestalotin). Of note, two bianthraquinones (i.e., skyrin and oxyskyrin) were detected in a culture extract of P. silybi. Two new chloroemodic acid derivatives (2-chloro-isorhodoptilometrin and 2-chloro-desmethyldermoquinone) isolated from the exudate of P. krskae ex-type culture were analyzed by nuclear magnetic resonance (NMR) and liquid chromatography–mass spectrometry (LC–MS). MDPI 2021-07-13 /pmc/articles/PMC8307998/ /pubmed/34356936 http://dx.doi.org/10.3390/jof7070557 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Labuda, Roman
Bacher, Markus
Rosenau, Thomas
Gasparotto, Erika
Gratzl, Hannes
Doppler, Maria
Sulyok, Michael
Kubátová, Alena
Berger, Harald
Cank, Kristof
Raja, Huzefa A.
Oberlies, Nicholas H.
Schüller, Christoph
Strauss, Joseph
Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.
title Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.
title_full Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.
title_fullStr Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.
title_full_unstemmed Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.
title_short Polyphasic Approach Utilized for the Identification of Two New Toxigenic Members of Penicillium Section Exilicaulis, P. krskae and P. silybi spp. nov.
title_sort polyphasic approach utilized for the identification of two new toxigenic members of penicillium section exilicaulis, p. krskae and p. silybi spp. nov.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307998/
https://www.ncbi.nlm.nih.gov/pubmed/34356936
http://dx.doi.org/10.3390/jof7070557
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