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Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet

The intestinal microbiome may trigger celiac disease (CD) in individuals with a genetic disposition when exposed to dietary gluten. Research demonstrates that nutrition during infancy is crucial to the intestinal microbiome engraftment. Very few studies to date have focused on the breast milk compos...

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Autores principales: Olshan, Katherine L., Zomorrodi, Ali R., Pujolassos, Meritxell, Troisi, Jacopo, Khan, Nayeim, Fanelli, Brian, Kenyon, Victoria, Fasano, Alessio, Leonard, Maureen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308312/
https://www.ncbi.nlm.nih.gov/pubmed/34210038
http://dx.doi.org/10.3390/nu13072243
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author Olshan, Katherine L.
Zomorrodi, Ali R.
Pujolassos, Meritxell
Troisi, Jacopo
Khan, Nayeim
Fanelli, Brian
Kenyon, Victoria
Fasano, Alessio
Leonard, Maureen M.
author_facet Olshan, Katherine L.
Zomorrodi, Ali R.
Pujolassos, Meritxell
Troisi, Jacopo
Khan, Nayeim
Fanelli, Brian
Kenyon, Victoria
Fasano, Alessio
Leonard, Maureen M.
author_sort Olshan, Katherine L.
collection PubMed
description The intestinal microbiome may trigger celiac disease (CD) in individuals with a genetic disposition when exposed to dietary gluten. Research demonstrates that nutrition during infancy is crucial to the intestinal microbiome engraftment. Very few studies to date have focused on the breast milk composition of subjects with a history of CD on a gluten-free diet. Here, we utilize a multi-omics approach with shotgun metagenomics to analyze the breast milk microbiome integrated with metabolome profiling of 36 subjects, 20 with CD on a gluten-free diet and 16 healthy controls. These analyses identified significant differences in bacterial and viral species/strains and functional pathways but no difference in metabolite abundance. Specifically, three bacterial strains with increased abundance were identified in subjects with CD on a gluten-free diet of which one (Rothia mucilaginosa) has been previously linked to autoimmune conditions. We also identified five pathways with increased abundance in subjects with CD on a gluten-free diet. We additionally found four bacterial and two viral species/strains with increased abundance in healthy controls. Overall, the differences observed in bacterial and viral species/strains and in functional pathways observed in our analysis may influence microbiome engraftment in neonates, which may impact their future clinical outcomes.
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spelling pubmed-83083122021-07-25 Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet Olshan, Katherine L. Zomorrodi, Ali R. Pujolassos, Meritxell Troisi, Jacopo Khan, Nayeim Fanelli, Brian Kenyon, Victoria Fasano, Alessio Leonard, Maureen M. Nutrients Article The intestinal microbiome may trigger celiac disease (CD) in individuals with a genetic disposition when exposed to dietary gluten. Research demonstrates that nutrition during infancy is crucial to the intestinal microbiome engraftment. Very few studies to date have focused on the breast milk composition of subjects with a history of CD on a gluten-free diet. Here, we utilize a multi-omics approach with shotgun metagenomics to analyze the breast milk microbiome integrated with metabolome profiling of 36 subjects, 20 with CD on a gluten-free diet and 16 healthy controls. These analyses identified significant differences in bacterial and viral species/strains and functional pathways but no difference in metabolite abundance. Specifically, three bacterial strains with increased abundance were identified in subjects with CD on a gluten-free diet of which one (Rothia mucilaginosa) has been previously linked to autoimmune conditions. We also identified five pathways with increased abundance in subjects with CD on a gluten-free diet. We additionally found four bacterial and two viral species/strains with increased abundance in healthy controls. Overall, the differences observed in bacterial and viral species/strains and in functional pathways observed in our analysis may influence microbiome engraftment in neonates, which may impact their future clinical outcomes. MDPI 2021-06-29 /pmc/articles/PMC8308312/ /pubmed/34210038 http://dx.doi.org/10.3390/nu13072243 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olshan, Katherine L.
Zomorrodi, Ali R.
Pujolassos, Meritxell
Troisi, Jacopo
Khan, Nayeim
Fanelli, Brian
Kenyon, Victoria
Fasano, Alessio
Leonard, Maureen M.
Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet
title Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet
title_full Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet
title_fullStr Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet
title_full_unstemmed Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet
title_short Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet
title_sort microbiota and metabolomic patterns in the breast milk of subjects with celiac disease on a gluten-free diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308312/
https://www.ncbi.nlm.nih.gov/pubmed/34210038
http://dx.doi.org/10.3390/nu13072243
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