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Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis

Glucose-based solutions remain the most used osmotic agents in peritoneal dialysis (PD), but unavoidably they contribute to the loss of peritoneal filtration capacity. Here, we evaluated at a molecular level the effects of XyloCore, a new PD solution with a low glucose content, in mesothelial and en...

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Autores principales: Masola, Valentina, Bonomini, Mario, Onisto, Maurizio, Ferraro, Pietro Manuel, Arduini, Arduino, Gambaro, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308380/
https://www.ncbi.nlm.nih.gov/pubmed/34209455
http://dx.doi.org/10.3390/nu13072282
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author Masola, Valentina
Bonomini, Mario
Onisto, Maurizio
Ferraro, Pietro Manuel
Arduini, Arduino
Gambaro, Giovanni
author_facet Masola, Valentina
Bonomini, Mario
Onisto, Maurizio
Ferraro, Pietro Manuel
Arduini, Arduino
Gambaro, Giovanni
author_sort Masola, Valentina
collection PubMed
description Glucose-based solutions remain the most used osmotic agents in peritoneal dialysis (PD), but unavoidably they contribute to the loss of peritoneal filtration capacity. Here, we evaluated at a molecular level the effects of XyloCore, a new PD solution with a low glucose content, in mesothelial and endothelial cells. Cell viability, integrity of mesothelial and endothelial cell membrane, activation of mesothelial and endothelial to mesenchymal transition programs, inflammation, and angiogenesis were evaluated by several techniques. Results showed that XyloCore preserves mesothelial and endothelial cell viability and membrane integrity. Moreover XyloCore, unlike glucose-based solutions, does not exert pro-fibrotic, -inflammatory, and -angiogenic effects. Overall, the in vitro evidence suggests that XyloCore could represent a potential biocompatible solution promising better outcomes in clinical practice.
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spelling pubmed-83083802021-07-25 Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis Masola, Valentina Bonomini, Mario Onisto, Maurizio Ferraro, Pietro Manuel Arduini, Arduino Gambaro, Giovanni Nutrients Article Glucose-based solutions remain the most used osmotic agents in peritoneal dialysis (PD), but unavoidably they contribute to the loss of peritoneal filtration capacity. Here, we evaluated at a molecular level the effects of XyloCore, a new PD solution with a low glucose content, in mesothelial and endothelial cells. Cell viability, integrity of mesothelial and endothelial cell membrane, activation of mesothelial and endothelial to mesenchymal transition programs, inflammation, and angiogenesis were evaluated by several techniques. Results showed that XyloCore preserves mesothelial and endothelial cell viability and membrane integrity. Moreover XyloCore, unlike glucose-based solutions, does not exert pro-fibrotic, -inflammatory, and -angiogenic effects. Overall, the in vitro evidence suggests that XyloCore could represent a potential biocompatible solution promising better outcomes in clinical practice. MDPI 2021-06-30 /pmc/articles/PMC8308380/ /pubmed/34209455 http://dx.doi.org/10.3390/nu13072282 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Masola, Valentina
Bonomini, Mario
Onisto, Maurizio
Ferraro, Pietro Manuel
Arduini, Arduino
Gambaro, Giovanni
Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis
title Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis
title_full Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis
title_fullStr Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis
title_full_unstemmed Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis
title_short Biological Effects of XyloCore, a Glucose Sparing PD Solution, on Mesothelial Cells: Focus on Mesothelial-Mesenchymal Transition, Inflammation and Angiogenesis
title_sort biological effects of xylocore, a glucose sparing pd solution, on mesothelial cells: focus on mesothelial-mesenchymal transition, inflammation and angiogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308380/
https://www.ncbi.nlm.nih.gov/pubmed/34209455
http://dx.doi.org/10.3390/nu13072282
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