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Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet

Background: A low-sodium (LS) diet reduces blood pressure, contributing to the prevention of cardiovascular diseases. However, intense dietary sodium restriction impairs insulin sensitivity and worsens lipid profile. Considering the benefits of aerobic exercise training (AET), the effect of LS diet...

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Autores principales: da Silva Ferreira, Guilherme, Bochi, Ana Paula Garcia, Pinto, Paula Ramos, Del Bianco, Vanessa, Rodrigues, Letícia Gomes, Morais, Mychel Raony Paiva Teixeira, Nakandakare, Edna Regina, Machado, Ubiratan Fabres, Catanozi, Sergio, Passarelli, Marisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308437/
https://www.ncbi.nlm.nih.gov/pubmed/34202724
http://dx.doi.org/10.3390/nu13072174
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author da Silva Ferreira, Guilherme
Bochi, Ana Paula Garcia
Pinto, Paula Ramos
Del Bianco, Vanessa
Rodrigues, Letícia Gomes
Morais, Mychel Raony Paiva Teixeira
Nakandakare, Edna Regina
Machado, Ubiratan Fabres
Catanozi, Sergio
Passarelli, Marisa
author_facet da Silva Ferreira, Guilherme
Bochi, Ana Paula Garcia
Pinto, Paula Ramos
Del Bianco, Vanessa
Rodrigues, Letícia Gomes
Morais, Mychel Raony Paiva Teixeira
Nakandakare, Edna Regina
Machado, Ubiratan Fabres
Catanozi, Sergio
Passarelli, Marisa
author_sort da Silva Ferreira, Guilherme
collection PubMed
description Background: A low-sodium (LS) diet reduces blood pressure, contributing to the prevention of cardiovascular diseases. However, intense dietary sodium restriction impairs insulin sensitivity and worsens lipid profile. Considering the benefits of aerobic exercise training (AET), the effect of LS diet and AET in hepatic lipid content and gene expression was investigated in LDL receptor knockout (LDLr-KO) mice. Methods: Twelve-week-old male LDLr-KO mice fed a normal sodium (NS) or LS diet were kept sedentary (S) or trained (T) for 90 days. Body mass, plasma lipids, insulin tolerance testing, hepatic triglyceride (TG) content, gene expression, and citrate synthase (CS) activity were determined. Results were compared by 2-way ANOVA and Tukey’s post-test. Results: Compared to NS, LS increased body mass and plasma TG, and impaired insulin sensitivity, which was prevented by AET. The LS-S group, but not the LS-T group, presented greater hepatic TG than the NS-S group. The LS diet increased the expression of genes related to insulin resistance (ApocIII, G6pc, Pck1) and reduced those involved in oxidative capacity (Prkaa1, Prkaa2, Ppara, Lipe) and lipoprotein assembly (Mttp). Conclusion: AET prevented the LS-diet-induced TG accumulation in the liver by improving insulin sensitivity and the expression of insulin-regulated genes and oxidative capacity.
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spelling pubmed-83084372021-07-25 Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet da Silva Ferreira, Guilherme Bochi, Ana Paula Garcia Pinto, Paula Ramos Del Bianco, Vanessa Rodrigues, Letícia Gomes Morais, Mychel Raony Paiva Teixeira Nakandakare, Edna Regina Machado, Ubiratan Fabres Catanozi, Sergio Passarelli, Marisa Nutrients Article Background: A low-sodium (LS) diet reduces blood pressure, contributing to the prevention of cardiovascular diseases. However, intense dietary sodium restriction impairs insulin sensitivity and worsens lipid profile. Considering the benefits of aerobic exercise training (AET), the effect of LS diet and AET in hepatic lipid content and gene expression was investigated in LDL receptor knockout (LDLr-KO) mice. Methods: Twelve-week-old male LDLr-KO mice fed a normal sodium (NS) or LS diet were kept sedentary (S) or trained (T) for 90 days. Body mass, plasma lipids, insulin tolerance testing, hepatic triglyceride (TG) content, gene expression, and citrate synthase (CS) activity were determined. Results were compared by 2-way ANOVA and Tukey’s post-test. Results: Compared to NS, LS increased body mass and plasma TG, and impaired insulin sensitivity, which was prevented by AET. The LS-S group, but not the LS-T group, presented greater hepatic TG than the NS-S group. The LS diet increased the expression of genes related to insulin resistance (ApocIII, G6pc, Pck1) and reduced those involved in oxidative capacity (Prkaa1, Prkaa2, Ppara, Lipe) and lipoprotein assembly (Mttp). Conclusion: AET prevented the LS-diet-induced TG accumulation in the liver by improving insulin sensitivity and the expression of insulin-regulated genes and oxidative capacity. MDPI 2021-06-24 /pmc/articles/PMC8308437/ /pubmed/34202724 http://dx.doi.org/10.3390/nu13072174 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva Ferreira, Guilherme
Bochi, Ana Paula Garcia
Pinto, Paula Ramos
Del Bianco, Vanessa
Rodrigues, Letícia Gomes
Morais, Mychel Raony Paiva Teixeira
Nakandakare, Edna Regina
Machado, Ubiratan Fabres
Catanozi, Sergio
Passarelli, Marisa
Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
title Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
title_full Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
title_fullStr Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
title_full_unstemmed Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
title_short Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
title_sort aerobic exercise training prevents insulin resistance and hepatic lipid accumulation in ldl receptor knockout mice chronically fed a low-sodium diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308437/
https://www.ncbi.nlm.nih.gov/pubmed/34202724
http://dx.doi.org/10.3390/nu13072174
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