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Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet
Background: A low-sodium (LS) diet reduces blood pressure, contributing to the prevention of cardiovascular diseases. However, intense dietary sodium restriction impairs insulin sensitivity and worsens lipid profile. Considering the benefits of aerobic exercise training (AET), the effect of LS diet...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308437/ https://www.ncbi.nlm.nih.gov/pubmed/34202724 http://dx.doi.org/10.3390/nu13072174 |
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author | da Silva Ferreira, Guilherme Bochi, Ana Paula Garcia Pinto, Paula Ramos Del Bianco, Vanessa Rodrigues, Letícia Gomes Morais, Mychel Raony Paiva Teixeira Nakandakare, Edna Regina Machado, Ubiratan Fabres Catanozi, Sergio Passarelli, Marisa |
author_facet | da Silva Ferreira, Guilherme Bochi, Ana Paula Garcia Pinto, Paula Ramos Del Bianco, Vanessa Rodrigues, Letícia Gomes Morais, Mychel Raony Paiva Teixeira Nakandakare, Edna Regina Machado, Ubiratan Fabres Catanozi, Sergio Passarelli, Marisa |
author_sort | da Silva Ferreira, Guilherme |
collection | PubMed |
description | Background: A low-sodium (LS) diet reduces blood pressure, contributing to the prevention of cardiovascular diseases. However, intense dietary sodium restriction impairs insulin sensitivity and worsens lipid profile. Considering the benefits of aerobic exercise training (AET), the effect of LS diet and AET in hepatic lipid content and gene expression was investigated in LDL receptor knockout (LDLr-KO) mice. Methods: Twelve-week-old male LDLr-KO mice fed a normal sodium (NS) or LS diet were kept sedentary (S) or trained (T) for 90 days. Body mass, plasma lipids, insulin tolerance testing, hepatic triglyceride (TG) content, gene expression, and citrate synthase (CS) activity were determined. Results were compared by 2-way ANOVA and Tukey’s post-test. Results: Compared to NS, LS increased body mass and plasma TG, and impaired insulin sensitivity, which was prevented by AET. The LS-S group, but not the LS-T group, presented greater hepatic TG than the NS-S group. The LS diet increased the expression of genes related to insulin resistance (ApocIII, G6pc, Pck1) and reduced those involved in oxidative capacity (Prkaa1, Prkaa2, Ppara, Lipe) and lipoprotein assembly (Mttp). Conclusion: AET prevented the LS-diet-induced TG accumulation in the liver by improving insulin sensitivity and the expression of insulin-regulated genes and oxidative capacity. |
format | Online Article Text |
id | pubmed-8308437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83084372021-07-25 Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet da Silva Ferreira, Guilherme Bochi, Ana Paula Garcia Pinto, Paula Ramos Del Bianco, Vanessa Rodrigues, Letícia Gomes Morais, Mychel Raony Paiva Teixeira Nakandakare, Edna Regina Machado, Ubiratan Fabres Catanozi, Sergio Passarelli, Marisa Nutrients Article Background: A low-sodium (LS) diet reduces blood pressure, contributing to the prevention of cardiovascular diseases. However, intense dietary sodium restriction impairs insulin sensitivity and worsens lipid profile. Considering the benefits of aerobic exercise training (AET), the effect of LS diet and AET in hepatic lipid content and gene expression was investigated in LDL receptor knockout (LDLr-KO) mice. Methods: Twelve-week-old male LDLr-KO mice fed a normal sodium (NS) or LS diet were kept sedentary (S) or trained (T) for 90 days. Body mass, plasma lipids, insulin tolerance testing, hepatic triglyceride (TG) content, gene expression, and citrate synthase (CS) activity were determined. Results were compared by 2-way ANOVA and Tukey’s post-test. Results: Compared to NS, LS increased body mass and plasma TG, and impaired insulin sensitivity, which was prevented by AET. The LS-S group, but not the LS-T group, presented greater hepatic TG than the NS-S group. The LS diet increased the expression of genes related to insulin resistance (ApocIII, G6pc, Pck1) and reduced those involved in oxidative capacity (Prkaa1, Prkaa2, Ppara, Lipe) and lipoprotein assembly (Mttp). Conclusion: AET prevented the LS-diet-induced TG accumulation in the liver by improving insulin sensitivity and the expression of insulin-regulated genes and oxidative capacity. MDPI 2021-06-24 /pmc/articles/PMC8308437/ /pubmed/34202724 http://dx.doi.org/10.3390/nu13072174 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article da Silva Ferreira, Guilherme Bochi, Ana Paula Garcia Pinto, Paula Ramos Del Bianco, Vanessa Rodrigues, Letícia Gomes Morais, Mychel Raony Paiva Teixeira Nakandakare, Edna Regina Machado, Ubiratan Fabres Catanozi, Sergio Passarelli, Marisa Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet |
title | Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet |
title_full | Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet |
title_fullStr | Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet |
title_full_unstemmed | Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet |
title_short | Aerobic Exercise Training Prevents Insulin Resistance and Hepatic Lipid Accumulation in LDL Receptor Knockout Mice Chronically Fed a Low-Sodium Diet |
title_sort | aerobic exercise training prevents insulin resistance and hepatic lipid accumulation in ldl receptor knockout mice chronically fed a low-sodium diet |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308437/ https://www.ncbi.nlm.nih.gov/pubmed/34202724 http://dx.doi.org/10.3390/nu13072174 |
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