Cargando…
Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
The ideal retrograde filling material that is easy to handle, has good physicochemical properties, and is biocompatible has not yet been developed. The current study reports the development of a novel bioactive glass based powder for use as a retrograde filling material that is capable of altering t...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308453/ https://www.ncbi.nlm.nih.gov/pubmed/34361212 http://dx.doi.org/10.3390/nano11071828 |
_version_ | 1783728284799860736 |
---|---|
author | Murata, Kazumasa Washio, Ayako Morotomi, Takahiko Rojasawasthien, Thira Kokabu, Shoichiro Kitamura, Chiaki |
author_facet | Murata, Kazumasa Washio, Ayako Morotomi, Takahiko Rojasawasthien, Thira Kokabu, Shoichiro Kitamura, Chiaki |
author_sort | Murata, Kazumasa |
collection | PubMed |
description | The ideal retrograde filling material that is easy to handle, has good physicochemical properties, and is biocompatible has not yet been developed. The current study reports the development of a novel bioactive glass based powder for use as a retrograde filling material that is capable of altering the consistency and hardening rate of mixtures when mixed with existing bioactive glass based cement. Furthermore, its physicochemical properties, in vitro effects on human cementoblast-like cells, and in vivo effects on inflammatory responses were evaluated. The surface of the hardened cement showed the formation of hydroxyapatite-like precipitates and calcium and silicate ions were eluted from the cement when the pH level was stabilized at 10.5. Additionally, the cement was found to be insoluble and exhibited favorable handling properties. No adverse effects on viability, proliferation, and expression of differentiated markers were observed in the in vitro experiment, and the cement was capable of inducing calcium deposition in the cells. Moreover, the cement demonstrated a lower number of infiltrated inflammatory cells compared to the other materials used in the in vivo mouse subcutaneous implantation experiment. These findings suggest that the retrograde filling material composed of bioactive glass and the novel bioactive glass based powder exhibits favorable physicochemical properties, cytocompatibility, and biocompatibility. |
format | Online Article Text |
id | pubmed-8308453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83084532021-07-25 Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material Murata, Kazumasa Washio, Ayako Morotomi, Takahiko Rojasawasthien, Thira Kokabu, Shoichiro Kitamura, Chiaki Nanomaterials (Basel) Article The ideal retrograde filling material that is easy to handle, has good physicochemical properties, and is biocompatible has not yet been developed. The current study reports the development of a novel bioactive glass based powder for use as a retrograde filling material that is capable of altering the consistency and hardening rate of mixtures when mixed with existing bioactive glass based cement. Furthermore, its physicochemical properties, in vitro effects on human cementoblast-like cells, and in vivo effects on inflammatory responses were evaluated. The surface of the hardened cement showed the formation of hydroxyapatite-like precipitates and calcium and silicate ions were eluted from the cement when the pH level was stabilized at 10.5. Additionally, the cement was found to be insoluble and exhibited favorable handling properties. No adverse effects on viability, proliferation, and expression of differentiated markers were observed in the in vitro experiment, and the cement was capable of inducing calcium deposition in the cells. Moreover, the cement demonstrated a lower number of infiltrated inflammatory cells compared to the other materials used in the in vivo mouse subcutaneous implantation experiment. These findings suggest that the retrograde filling material composed of bioactive glass and the novel bioactive glass based powder exhibits favorable physicochemical properties, cytocompatibility, and biocompatibility. MDPI 2021-07-14 /pmc/articles/PMC8308453/ /pubmed/34361212 http://dx.doi.org/10.3390/nano11071828 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Murata, Kazumasa Washio, Ayako Morotomi, Takahiko Rojasawasthien, Thira Kokabu, Shoichiro Kitamura, Chiaki Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material |
title | Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material |
title_full | Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material |
title_fullStr | Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material |
title_full_unstemmed | Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material |
title_short | Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material |
title_sort | physicochemical properties, cytocompatibility, and biocompatibility of a bioactive glass based retrograde filling material |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308453/ https://www.ncbi.nlm.nih.gov/pubmed/34361212 http://dx.doi.org/10.3390/nano11071828 |
work_keys_str_mv | AT muratakazumasa physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial AT washioayako physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial AT morotomitakahiko physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial AT rojasawasthienthira physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial AT kokabushoichiro physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial AT kitamurachiaki physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial |