Cargando…

Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material

The ideal retrograde filling material that is easy to handle, has good physicochemical properties, and is biocompatible has not yet been developed. The current study reports the development of a novel bioactive glass based powder for use as a retrograde filling material that is capable of altering t...

Descripción completa

Detalles Bibliográficos
Autores principales: Murata, Kazumasa, Washio, Ayako, Morotomi, Takahiko, Rojasawasthien, Thira, Kokabu, Shoichiro, Kitamura, Chiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308453/
https://www.ncbi.nlm.nih.gov/pubmed/34361212
http://dx.doi.org/10.3390/nano11071828
_version_ 1783728284799860736
author Murata, Kazumasa
Washio, Ayako
Morotomi, Takahiko
Rojasawasthien, Thira
Kokabu, Shoichiro
Kitamura, Chiaki
author_facet Murata, Kazumasa
Washio, Ayako
Morotomi, Takahiko
Rojasawasthien, Thira
Kokabu, Shoichiro
Kitamura, Chiaki
author_sort Murata, Kazumasa
collection PubMed
description The ideal retrograde filling material that is easy to handle, has good physicochemical properties, and is biocompatible has not yet been developed. The current study reports the development of a novel bioactive glass based powder for use as a retrograde filling material that is capable of altering the consistency and hardening rate of mixtures when mixed with existing bioactive glass based cement. Furthermore, its physicochemical properties, in vitro effects on human cementoblast-like cells, and in vivo effects on inflammatory responses were evaluated. The surface of the hardened cement showed the formation of hydroxyapatite-like precipitates and calcium and silicate ions were eluted from the cement when the pH level was stabilized at 10.5. Additionally, the cement was found to be insoluble and exhibited favorable handling properties. No adverse effects on viability, proliferation, and expression of differentiated markers were observed in the in vitro experiment, and the cement was capable of inducing calcium deposition in the cells. Moreover, the cement demonstrated a lower number of infiltrated inflammatory cells compared to the other materials used in the in vivo mouse subcutaneous implantation experiment. These findings suggest that the retrograde filling material composed of bioactive glass and the novel bioactive glass based powder exhibits favorable physicochemical properties, cytocompatibility, and biocompatibility.
format Online
Article
Text
id pubmed-8308453
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83084532021-07-25 Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material Murata, Kazumasa Washio, Ayako Morotomi, Takahiko Rojasawasthien, Thira Kokabu, Shoichiro Kitamura, Chiaki Nanomaterials (Basel) Article The ideal retrograde filling material that is easy to handle, has good physicochemical properties, and is biocompatible has not yet been developed. The current study reports the development of a novel bioactive glass based powder for use as a retrograde filling material that is capable of altering the consistency and hardening rate of mixtures when mixed with existing bioactive glass based cement. Furthermore, its physicochemical properties, in vitro effects on human cementoblast-like cells, and in vivo effects on inflammatory responses were evaluated. The surface of the hardened cement showed the formation of hydroxyapatite-like precipitates and calcium and silicate ions were eluted from the cement when the pH level was stabilized at 10.5. Additionally, the cement was found to be insoluble and exhibited favorable handling properties. No adverse effects on viability, proliferation, and expression of differentiated markers were observed in the in vitro experiment, and the cement was capable of inducing calcium deposition in the cells. Moreover, the cement demonstrated a lower number of infiltrated inflammatory cells compared to the other materials used in the in vivo mouse subcutaneous implantation experiment. These findings suggest that the retrograde filling material composed of bioactive glass and the novel bioactive glass based powder exhibits favorable physicochemical properties, cytocompatibility, and biocompatibility. MDPI 2021-07-14 /pmc/articles/PMC8308453/ /pubmed/34361212 http://dx.doi.org/10.3390/nano11071828 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murata, Kazumasa
Washio, Ayako
Morotomi, Takahiko
Rojasawasthien, Thira
Kokabu, Shoichiro
Kitamura, Chiaki
Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
title Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
title_full Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
title_fullStr Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
title_full_unstemmed Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
title_short Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material
title_sort physicochemical properties, cytocompatibility, and biocompatibility of a bioactive glass based retrograde filling material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308453/
https://www.ncbi.nlm.nih.gov/pubmed/34361212
http://dx.doi.org/10.3390/nano11071828
work_keys_str_mv AT muratakazumasa physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial
AT washioayako physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial
AT morotomitakahiko physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial
AT rojasawasthienthira physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial
AT kokabushoichiro physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial
AT kitamurachiaki physicochemicalpropertiescytocompatibilityandbiocompatibilityofabioactiveglassbasedretrogradefillingmaterial