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Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds

Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed he...

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Autores principales: Bilyy, Rostyslav, Pagneux, Quentin, François, Nathan, Bila, Galyna, Grytsko, Roman, Lebedin, Yuri, Barras, Alexandre, Dubuisson, Jean, Belouzard, Sandrine, Séron, Karin, Boukherroub, Rabah, Szunerits, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308543/
https://www.ncbi.nlm.nih.gov/pubmed/34358011
http://dx.doi.org/10.3390/pathogens10070861
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author Bilyy, Rostyslav
Pagneux, Quentin
François, Nathan
Bila, Galyna
Grytsko, Roman
Lebedin, Yuri
Barras, Alexandre
Dubuisson, Jean
Belouzard, Sandrine
Séron, Karin
Boukherroub, Rabah
Szunerits, Sabine
author_facet Bilyy, Rostyslav
Pagneux, Quentin
François, Nathan
Bila, Galyna
Grytsko, Roman
Lebedin, Yuri
Barras, Alexandre
Dubuisson, Jean
Belouzard, Sandrine
Séron, Karin
Boukherroub, Rabah
Szunerits, Sabine
author_sort Bilyy, Rostyslav
collection PubMed
description Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed here as a rapid and versatile platform for antigen conjugation, utilizing peptides common to different pathogenic strains and making this strategy a good candidate for a “ready-to-use” vaccine. Initiation of an inflammatory reaction with a resulting immune response is based on the ability of living organisms to entrap nanostructures such as nanodiamonds with neutrophil extracellular traps (NETs) formation. In this work, coronavirus peptide homological for MERS-CoV, fusion inhibitor, was conjugated to nanodiamonds and used to induce neutrophilic-driven self-limiting inflammation. The resulting adjuvant was safe and did not induce any tissue damage at the site of injection. Mice immunization resulted in IgG titers of ¼,000 within 28 days. Immunization of rabbits resulted in the formation of a high level of antibodies persistently present for up to 120 days after the first immunization (animal lifespan ~3 years). The peptide used for immunization proved to be reactive with sera of convalescent COVID patients, demonstrating the possibility of developing pancoronaviral vaccine candidates.
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spelling pubmed-83085432021-07-25 Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds Bilyy, Rostyslav Pagneux, Quentin François, Nathan Bila, Galyna Grytsko, Roman Lebedin, Yuri Barras, Alexandre Dubuisson, Jean Belouzard, Sandrine Séron, Karin Boukherroub, Rabah Szunerits, Sabine Pathogens Article Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed here as a rapid and versatile platform for antigen conjugation, utilizing peptides common to different pathogenic strains and making this strategy a good candidate for a “ready-to-use” vaccine. Initiation of an inflammatory reaction with a resulting immune response is based on the ability of living organisms to entrap nanostructures such as nanodiamonds with neutrophil extracellular traps (NETs) formation. In this work, coronavirus peptide homological for MERS-CoV, fusion inhibitor, was conjugated to nanodiamonds and used to induce neutrophilic-driven self-limiting inflammation. The resulting adjuvant was safe and did not induce any tissue damage at the site of injection. Mice immunization resulted in IgG titers of ¼,000 within 28 days. Immunization of rabbits resulted in the formation of a high level of antibodies persistently present for up to 120 days after the first immunization (animal lifespan ~3 years). The peptide used for immunization proved to be reactive with sera of convalescent COVID patients, demonstrating the possibility of developing pancoronaviral vaccine candidates. MDPI 2021-07-08 /pmc/articles/PMC8308543/ /pubmed/34358011 http://dx.doi.org/10.3390/pathogens10070861 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bilyy, Rostyslav
Pagneux, Quentin
François, Nathan
Bila, Galyna
Grytsko, Roman
Lebedin, Yuri
Barras, Alexandre
Dubuisson, Jean
Belouzard, Sandrine
Séron, Karin
Boukherroub, Rabah
Szunerits, Sabine
Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds
title Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds
title_full Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds
title_fullStr Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds
title_full_unstemmed Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds
title_short Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified Nanodiamonds
title_sort rapid generation of coronaviral immunity using recombinant peptide modified nanodiamonds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308543/
https://www.ncbi.nlm.nih.gov/pubmed/34358011
http://dx.doi.org/10.3390/pathogens10070861
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