Cargando…

Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis

Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and co...

Descripción completa

Detalles Bibliográficos
Autores principales: Pacheco, Iván, Díaz-Sánchez, Sandra, Contreras, Marinela, Villar, Margarita, Cabezas-Cruz, Alejandro, Gortázar, Christian, de la Fuente, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308674/
https://www.ncbi.nlm.nih.gov/pubmed/34208966
http://dx.doi.org/10.3390/ph14070635
_version_ 1783728337443618816
author Pacheco, Iván
Díaz-Sánchez, Sandra
Contreras, Marinela
Villar, Margarita
Cabezas-Cruz, Alejandro
Gortázar, Christian
de la Fuente, José
author_facet Pacheco, Iván
Díaz-Sánchez, Sandra
Contreras, Marinela
Villar, Margarita
Cabezas-Cruz, Alejandro
Gortázar, Christian
de la Fuente, José
author_sort Pacheco, Iván
collection PubMed
description Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water quality. However, the identification and characterization of the molecules and mechanisms associated with probiotics is a challenge that requires investigation. To address this challenge, herein we used the zebrafish model for the study of the efficacy and mechanisms of probiotic interventions against tuberculosis. First, bacteria from fish gut microbiota were identified with high content of the surface glycotope Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) that has been shown to induce protective immune responses. The results showed that probiotics of selected bacteria with high α-Gal content, namely Aeromonas veronii and Pseudomonas entomophila, were biosafe and effective for the control of Mycobacterium marinum. Protective mechanisms regulating immunity and metabolism activated in response to α-Gal and probiotics with high α-Gal content included modification of gut microbiota composition, B-cell maturation, anti-α-Gal antibodies-mediated control of mycobacteria, induced innate immune responses, beneficial effects on nutrient metabolism and reduced oxidative stress. These results support the potential of probiotics with high α-Gal content for the control of fish mycobacteriosis and suggested the possibility of exploring the development of combined probiotic treatments alone and in combination with α-Gal for the control of infectious diseases.
format Online
Article
Text
id pubmed-8308674
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83086742021-07-25 Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis Pacheco, Iván Díaz-Sánchez, Sandra Contreras, Marinela Villar, Margarita Cabezas-Cruz, Alejandro Gortázar, Christian de la Fuente, José Pharmaceuticals (Basel) Article Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water quality. However, the identification and characterization of the molecules and mechanisms associated with probiotics is a challenge that requires investigation. To address this challenge, herein we used the zebrafish model for the study of the efficacy and mechanisms of probiotic interventions against tuberculosis. First, bacteria from fish gut microbiota were identified with high content of the surface glycotope Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) that has been shown to induce protective immune responses. The results showed that probiotics of selected bacteria with high α-Gal content, namely Aeromonas veronii and Pseudomonas entomophila, were biosafe and effective for the control of Mycobacterium marinum. Protective mechanisms regulating immunity and metabolism activated in response to α-Gal and probiotics with high α-Gal content included modification of gut microbiota composition, B-cell maturation, anti-α-Gal antibodies-mediated control of mycobacteria, induced innate immune responses, beneficial effects on nutrient metabolism and reduced oxidative stress. These results support the potential of probiotics with high α-Gal content for the control of fish mycobacteriosis and suggested the possibility of exploring the development of combined probiotic treatments alone and in combination with α-Gal for the control of infectious diseases. MDPI 2021-06-30 /pmc/articles/PMC8308674/ /pubmed/34208966 http://dx.doi.org/10.3390/ph14070635 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pacheco, Iván
Díaz-Sánchez, Sandra
Contreras, Marinela
Villar, Margarita
Cabezas-Cruz, Alejandro
Gortázar, Christian
de la Fuente, José
Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis
title Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis
title_full Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis
title_fullStr Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis
title_full_unstemmed Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis
title_short Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis
title_sort probiotic bacteria with high alpha-gal content protect zebrafish against mycobacteriosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308674/
https://www.ncbi.nlm.nih.gov/pubmed/34208966
http://dx.doi.org/10.3390/ph14070635
work_keys_str_mv AT pachecoivan probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis
AT diazsanchezsandra probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis
AT contrerasmarinela probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis
AT villarmargarita probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis
AT cabezascruzalejandro probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis
AT gortazarchristian probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis
AT delafuentejose probioticbacteriawithhighalphagalcontentprotectzebrafishagainstmycobacteriosis