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Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices
The fabrication of fiber mats via electrospinning has been adopted in the last decades to produce high quality scaffolds for tissue engineering. However, an effective combination of electrospinning methods with gene delivery therapies remains a challenge. In this study, we describe how the delivery...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308744/ https://www.ncbi.nlm.nih.gov/pubmed/34358092 http://dx.doi.org/10.3390/ph14070666 |
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author | Tsekoura, Eleni K. Dick, Teo Pankongadisak, Porntipa Graf, Daniel Boluk, Yaman Uludağ, Hasan |
author_facet | Tsekoura, Eleni K. Dick, Teo Pankongadisak, Porntipa Graf, Daniel Boluk, Yaman Uludağ, Hasan |
author_sort | Tsekoura, Eleni K. |
collection | PubMed |
description | The fabrication of fiber mats via electrospinning has been adopted in the last decades to produce high quality scaffolds for tissue engineering. However, an effective combination of electrospinning methods with gene delivery therapies remains a challenge. In this study, we describe how the delivery of gene complexes via electrospun mats that contain different volumes of gelatin (Gel), collagen (Col), and polyethylene glycol (PEG) can affect gene expression by transfected cells. Non-viral complexes were formulated by using lipid modified polyethylenimine (PEI) polymer and plasmid DNAs (pDNA) like the reporter Green Fluorescent Protein (GFP) and the therapeutically relevant Bone Morphogenetic Protein-2 (BMP-2) and electrospuned after being mixed with different volumes of Gel-Col-PEG mats and delivered to human myoblast (C2C12) and mouse osteoblast cells (MC3T3). The entrapment of GFP complexes via different homogeneous electrospun fiber mats revealed that a high fraction of collagen in the mats affected the quality of the fibers and led to reduced transfection efficiency on target cells. On the other hand, the fabrication of double-layered mats that contained collagen without complexes as a first layer and gelatin-collagen-PEG with complexes as a second layer successfully induced GFP expression and ALP activity in C2C12 cells. We conclude that this study has established the advantage of formulating multilayered bioactive collagen-based mats for gene delivery applications. |
format | Online Article Text |
id | pubmed-8308744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83087442021-07-25 Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices Tsekoura, Eleni K. Dick, Teo Pankongadisak, Porntipa Graf, Daniel Boluk, Yaman Uludağ, Hasan Pharmaceuticals (Basel) Article The fabrication of fiber mats via electrospinning has been adopted in the last decades to produce high quality scaffolds for tissue engineering. However, an effective combination of electrospinning methods with gene delivery therapies remains a challenge. In this study, we describe how the delivery of gene complexes via electrospun mats that contain different volumes of gelatin (Gel), collagen (Col), and polyethylene glycol (PEG) can affect gene expression by transfected cells. Non-viral complexes were formulated by using lipid modified polyethylenimine (PEI) polymer and plasmid DNAs (pDNA) like the reporter Green Fluorescent Protein (GFP) and the therapeutically relevant Bone Morphogenetic Protein-2 (BMP-2) and electrospuned after being mixed with different volumes of Gel-Col-PEG mats and delivered to human myoblast (C2C12) and mouse osteoblast cells (MC3T3). The entrapment of GFP complexes via different homogeneous electrospun fiber mats revealed that a high fraction of collagen in the mats affected the quality of the fibers and led to reduced transfection efficiency on target cells. On the other hand, the fabrication of double-layered mats that contained collagen without complexes as a first layer and gelatin-collagen-PEG with complexes as a second layer successfully induced GFP expression and ALP activity in C2C12 cells. We conclude that this study has established the advantage of formulating multilayered bioactive collagen-based mats for gene delivery applications. MDPI 2021-07-12 /pmc/articles/PMC8308744/ /pubmed/34358092 http://dx.doi.org/10.3390/ph14070666 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsekoura, Eleni K. Dick, Teo Pankongadisak, Porntipa Graf, Daniel Boluk, Yaman Uludağ, Hasan Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices |
title | Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices |
title_full | Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices |
title_fullStr | Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices |
title_full_unstemmed | Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices |
title_short | Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices |
title_sort | delivery of bioactive gene particles via gelatin-collagen-peg-based electrospun matrices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308744/ https://www.ncbi.nlm.nih.gov/pubmed/34358092 http://dx.doi.org/10.3390/ph14070666 |
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