Cargando…
α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats
Excessive liver lipid deposition is a vital risk factor for the development of many diseases. Here, we fed Sprague-Dawley rats with a control or α-lipoic acid-supplemented diet (0.2%) for 5 weeks to elucidate the effects of α-lipoic acid on preventive ability, hepatic lipid metabolism-related gene e...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308747/ https://www.ncbi.nlm.nih.gov/pubmed/34371841 http://dx.doi.org/10.3390/nu13072331 |
_version_ | 1783728355261022208 |
---|---|
author | Guo, Shihui Yan, Kai Fang, Xi Ni, Yingdong Ma, Wenqiang Zhao, Ruqian |
author_facet | Guo, Shihui Yan, Kai Fang, Xi Ni, Yingdong Ma, Wenqiang Zhao, Ruqian |
author_sort | Guo, Shihui |
collection | PubMed |
description | Excessive liver lipid deposition is a vital risk factor for the development of many diseases. Here, we fed Sprague-Dawley rats with a control or α-lipoic acid-supplemented diet (0.2%) for 5 weeks to elucidate the effects of α-lipoic acid on preventive ability, hepatic lipid metabolism-related gene expression, and the involved regulatory mechanisms. In the current study, α-lipoic acid supplementation lowered plasma triglyceride level and hepatic triglyceride content. Reduced hepatic lipid deposition was closely associated with inhibiting fatty acid-binding protein 1 and fatty acid synthase expression, as well as increasing phosphorylated hormone-sensitive lipase expression at the protein level in α-lipoic acid-exposed rats. Hepatic miRNA sequencing revealed increased expression of miR-3548 targeting the 3′untranslated region of Fasn mRNA, and the direct regulatory link between miRNA-3548 and FASN was verified by dual-luciferase reporter assay. Taken together, α-lipoic acid lowered hepatic lipid accumulation, which involved changes in miRNA-mediated lipogenic genes. |
format | Online Article Text |
id | pubmed-8308747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83087472021-07-25 α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats Guo, Shihui Yan, Kai Fang, Xi Ni, Yingdong Ma, Wenqiang Zhao, Ruqian Nutrients Article Excessive liver lipid deposition is a vital risk factor for the development of many diseases. Here, we fed Sprague-Dawley rats with a control or α-lipoic acid-supplemented diet (0.2%) for 5 weeks to elucidate the effects of α-lipoic acid on preventive ability, hepatic lipid metabolism-related gene expression, and the involved regulatory mechanisms. In the current study, α-lipoic acid supplementation lowered plasma triglyceride level and hepatic triglyceride content. Reduced hepatic lipid deposition was closely associated with inhibiting fatty acid-binding protein 1 and fatty acid synthase expression, as well as increasing phosphorylated hormone-sensitive lipase expression at the protein level in α-lipoic acid-exposed rats. Hepatic miRNA sequencing revealed increased expression of miR-3548 targeting the 3′untranslated region of Fasn mRNA, and the direct regulatory link between miRNA-3548 and FASN was verified by dual-luciferase reporter assay. Taken together, α-lipoic acid lowered hepatic lipid accumulation, which involved changes in miRNA-mediated lipogenic genes. MDPI 2021-07-08 /pmc/articles/PMC8308747/ /pubmed/34371841 http://dx.doi.org/10.3390/nu13072331 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guo, Shihui Yan, Kai Fang, Xi Ni, Yingdong Ma, Wenqiang Zhao, Ruqian α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats |
title | α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats |
title_full | α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats |
title_fullStr | α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats |
title_full_unstemmed | α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats |
title_short | α-Lipoic Acid Alleviates Hepatic Lipid Deposition by Inhibiting FASN Expression via miR-3548 in Rats |
title_sort | α-lipoic acid alleviates hepatic lipid deposition by inhibiting fasn expression via mir-3548 in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308747/ https://www.ncbi.nlm.nih.gov/pubmed/34371841 http://dx.doi.org/10.3390/nu13072331 |
work_keys_str_mv | AT guoshihui alipoicacidalleviateshepaticlipiddepositionbyinhibitingfasnexpressionviamir3548inrats AT yankai alipoicacidalleviateshepaticlipiddepositionbyinhibitingfasnexpressionviamir3548inrats AT fangxi alipoicacidalleviateshepaticlipiddepositionbyinhibitingfasnexpressionviamir3548inrats AT niyingdong alipoicacidalleviateshepaticlipiddepositionbyinhibitingfasnexpressionviamir3548inrats AT mawenqiang alipoicacidalleviateshepaticlipiddepositionbyinhibitingfasnexpressionviamir3548inrats AT zhaoruqian alipoicacidalleviateshepaticlipiddepositionbyinhibitingfasnexpressionviamir3548inrats |