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Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity

Real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to achieve a sensitivity of 1 parasite/mL. For this purpose, we cloned the conserved kDNA fragment of 120 bp into competent cells and correlated them with serial dilutions of DNA extracted from reference parasi...

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Detalles Bibliográficos
Autores principales:	Castelli, Germano, Bruno, Federica, Reale, Stefano, Catanzaro, Simone, Valenza, Viviana, Vitale, Fabrizio
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308825/ https://www.ncbi.nlm.nih.gov/pubmed/34358015 http://dx.doi.org/10.3390/pathogens10070865

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