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Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts

In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rod...

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Autores principales: Lücken, Anneke, Wente, Nicole, Zhang, Yanchao, Woudstra, Svenja, Krömker, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308849/
https://www.ncbi.nlm.nih.gov/pubmed/34357981
http://dx.doi.org/10.3390/pathogens10070831
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author Lücken, Anneke
Wente, Nicole
Zhang, Yanchao
Woudstra, Svenja
Krömker, Volker
author_facet Lücken, Anneke
Wente, Nicole
Zhang, Yanchao
Woudstra, Svenja
Krömker, Volker
author_sort Lücken, Anneke
collection PubMed
description In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rods were differentiated using the direct transfer method in MALDI-TOF MS. C. bovis, C. amycolatum, C. xerosis, and five other species were identified with proportions of 90.1%, 7.7%, and 0.8% for the named species, respectively, and 1.4% for the remaining unnamed species. In addition, somatic cell count (SCC) was determined by flow cytometry. Based on this, the isolates were classified into four udder health groups: “latent infection”, “subclinical mastitis”, “clinical mastitis” and “others”. Approximately 90% of isolates of C. bovis and C. amycolatum were from latently and subclinically infected quarters. Of the C. bovis isolates, 5.8% were obtained from milk samples from clinical mastitis, whereas C. amycolatum was not present in clinical mastitis. The distribution of groups in these two species differed significantly. The geometric mean SCC of all species combined was 76,000 SCC/mL, almost the same as the SCC of C. bovis. With 50,000 SCC/mL, the SCC of C. amycolatum was slightly below the SCC of C. bovis. Through the species-level detection and consideration of SCC performed here, it is apparent that individual species differ in terms of their pathogenicity. Overall, their classification as minor pathogens with an SCC increase is confirmed.
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spelling pubmed-83088492021-07-25 Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts Lücken, Anneke Wente, Nicole Zhang, Yanchao Woudstra, Svenja Krömker, Volker Pathogens Article In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rods were differentiated using the direct transfer method in MALDI-TOF MS. C. bovis, C. amycolatum, C. xerosis, and five other species were identified with proportions of 90.1%, 7.7%, and 0.8% for the named species, respectively, and 1.4% for the remaining unnamed species. In addition, somatic cell count (SCC) was determined by flow cytometry. Based on this, the isolates were classified into four udder health groups: “latent infection”, “subclinical mastitis”, “clinical mastitis” and “others”. Approximately 90% of isolates of C. bovis and C. amycolatum were from latently and subclinically infected quarters. Of the C. bovis isolates, 5.8% were obtained from milk samples from clinical mastitis, whereas C. amycolatum was not present in clinical mastitis. The distribution of groups in these two species differed significantly. The geometric mean SCC of all species combined was 76,000 SCC/mL, almost the same as the SCC of C. bovis. With 50,000 SCC/mL, the SCC of C. amycolatum was slightly below the SCC of C. bovis. Through the species-level detection and consideration of SCC performed here, it is apparent that individual species differ in terms of their pathogenicity. Overall, their classification as minor pathogens with an SCC increase is confirmed. MDPI 2021-07-02 /pmc/articles/PMC8308849/ /pubmed/34357981 http://dx.doi.org/10.3390/pathogens10070831 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lücken, Anneke
Wente, Nicole
Zhang, Yanchao
Woudstra, Svenja
Krömker, Volker
Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
title Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
title_full Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
title_fullStr Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
title_full_unstemmed Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
title_short Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
title_sort corynebacteria in bovine quarter milk samples—species and somatic cell counts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308849/
https://www.ncbi.nlm.nih.gov/pubmed/34357981
http://dx.doi.org/10.3390/pathogens10070831
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