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Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rod...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308849/ https://www.ncbi.nlm.nih.gov/pubmed/34357981 http://dx.doi.org/10.3390/pathogens10070831 |
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author | Lücken, Anneke Wente, Nicole Zhang, Yanchao Woudstra, Svenja Krömker, Volker |
author_facet | Lücken, Anneke Wente, Nicole Zhang, Yanchao Woudstra, Svenja Krömker, Volker |
author_sort | Lücken, Anneke |
collection | PubMed |
description | In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rods were differentiated using the direct transfer method in MALDI-TOF MS. C. bovis, C. amycolatum, C. xerosis, and five other species were identified with proportions of 90.1%, 7.7%, and 0.8% for the named species, respectively, and 1.4% for the remaining unnamed species. In addition, somatic cell count (SCC) was determined by flow cytometry. Based on this, the isolates were classified into four udder health groups: “latent infection”, “subclinical mastitis”, “clinical mastitis” and “others”. Approximately 90% of isolates of C. bovis and C. amycolatum were from latently and subclinically infected quarters. Of the C. bovis isolates, 5.8% were obtained from milk samples from clinical mastitis, whereas C. amycolatum was not present in clinical mastitis. The distribution of groups in these two species differed significantly. The geometric mean SCC of all species combined was 76,000 SCC/mL, almost the same as the SCC of C. bovis. With 50,000 SCC/mL, the SCC of C. amycolatum was slightly below the SCC of C. bovis. Through the species-level detection and consideration of SCC performed here, it is apparent that individual species differ in terms of their pathogenicity. Overall, their classification as minor pathogens with an SCC increase is confirmed. |
format | Online Article Text |
id | pubmed-8308849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83088492021-07-25 Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts Lücken, Anneke Wente, Nicole Zhang, Yanchao Woudstra, Svenja Krömker, Volker Pathogens Article In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rods were differentiated using the direct transfer method in MALDI-TOF MS. C. bovis, C. amycolatum, C. xerosis, and five other species were identified with proportions of 90.1%, 7.7%, and 0.8% for the named species, respectively, and 1.4% for the remaining unnamed species. In addition, somatic cell count (SCC) was determined by flow cytometry. Based on this, the isolates were classified into four udder health groups: “latent infection”, “subclinical mastitis”, “clinical mastitis” and “others”. Approximately 90% of isolates of C. bovis and C. amycolatum were from latently and subclinically infected quarters. Of the C. bovis isolates, 5.8% were obtained from milk samples from clinical mastitis, whereas C. amycolatum was not present in clinical mastitis. The distribution of groups in these two species differed significantly. The geometric mean SCC of all species combined was 76,000 SCC/mL, almost the same as the SCC of C. bovis. With 50,000 SCC/mL, the SCC of C. amycolatum was slightly below the SCC of C. bovis. Through the species-level detection and consideration of SCC performed here, it is apparent that individual species differ in terms of their pathogenicity. Overall, their classification as minor pathogens with an SCC increase is confirmed. MDPI 2021-07-02 /pmc/articles/PMC8308849/ /pubmed/34357981 http://dx.doi.org/10.3390/pathogens10070831 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lücken, Anneke Wente, Nicole Zhang, Yanchao Woudstra, Svenja Krömker, Volker Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts |
title | Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts |
title_full | Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts |
title_fullStr | Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts |
title_full_unstemmed | Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts |
title_short | Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts |
title_sort | corynebacteria in bovine quarter milk samples—species and somatic cell counts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308849/ https://www.ncbi.nlm.nih.gov/pubmed/34357981 http://dx.doi.org/10.3390/pathogens10070831 |
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