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Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol

Background: The protocol study will focus on the seroprevalence of IgG antibodies to SARS-CoV-2 achieved by vaccination and/or natural protection as well as the history, symptoms, and risk factors for SARS-CoV-2 in four primary health-care workers (PHCWs) and their household contacts in metropolitan...

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Autores principales: Pouquet, Marie, Decarreaux, Dorine, Prévot-Monsacré, Pol, Hervé, Corentin, Werner, Andréas, Grosgogeat, Brigitte, Blanché, Hélène, Rabiega, Pascaline, Laupie, Julien, Kochert, Fabienne, Abraham, Nathalie, Sebaoun, Jean-Marc, de Lamballerie, Xavier, Charrel, Remi, Souty, Cecile, Camara, Ibrahima, Pergeline, Jeanne, Noël, Harold, Guerrisi, Caroline, van der Werf, Sylvie, Carrat, Fabrice, Hanslik, Thomas, Blanchon, Thierry, Falchi, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308883/
https://www.ncbi.nlm.nih.gov/pubmed/34358061
http://dx.doi.org/10.3390/pathogens10070911
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author Pouquet, Marie
Decarreaux, Dorine
Prévot-Monsacré, Pol
Hervé, Corentin
Werner, Andréas
Grosgogeat, Brigitte
Blanché, Hélène
Rabiega, Pascaline
Laupie, Julien
Kochert, Fabienne
Abraham, Nathalie
Sebaoun, Jean-Marc
de Lamballerie, Xavier
Charrel, Remi
Souty, Cecile
Camara, Ibrahima
Pergeline, Jeanne
Noël, Harold
Guerrisi, Caroline
van der Werf, Sylvie
Carrat, Fabrice
Hanslik, Thomas
Blanchon, Thierry
Falchi, Alessandra
author_facet Pouquet, Marie
Decarreaux, Dorine
Prévot-Monsacré, Pol
Hervé, Corentin
Werner, Andréas
Grosgogeat, Brigitte
Blanché, Hélène
Rabiega, Pascaline
Laupie, Julien
Kochert, Fabienne
Abraham, Nathalie
Sebaoun, Jean-Marc
de Lamballerie, Xavier
Charrel, Remi
Souty, Cecile
Camara, Ibrahima
Pergeline, Jeanne
Noël, Harold
Guerrisi, Caroline
van der Werf, Sylvie
Carrat, Fabrice
Hanslik, Thomas
Blanchon, Thierry
Falchi, Alessandra
author_sort Pouquet, Marie
collection PubMed
description Background: The protocol study will focus on the seroprevalence of IgG antibodies to SARS-CoV-2 achieved by vaccination and/or natural protection as well as the history, symptoms, and risk factors for SARS-CoV-2 in four primary health-care workers (PHCWs) and their household contacts in metropolitan France. Methods: Here, we propose a protocol for a nationwide survey to determine the seroprevalence of IgG antibodies to SARS-CoV-2 achieved by vaccination and/or natural protection in four PHCW populations (general practitioners, pediatricians, pharmacists and assistants, and dentists and assistants) and their household contacts. Participants will be included from June to July 2021 (Phase 1) among PHCW populations located throughout metropolitan France. They will be asked to provide a range of demographic and behavioral information since the first SARS-CoV-2 wave and a self-sampled dried blood spot. Phase 1 will involve also a questionnaire and serological study of PHCWs’ household contacts. Seroprevalence will be estimated using two ELISAs designed to detect specific IgG antibodies to SARS-CoV-2 in humoral fluid, and these results will be confirmed using a virus neutralization test. This study will be repeated from November to December 2021 (Phase 2) to evaluate the evolution of immune status achieved by vaccination and/or natural protection of PHCWs and to describe the history of exposure to SARS-CoV-2.
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spelling pubmed-83088832021-07-25 Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol Pouquet, Marie Decarreaux, Dorine Prévot-Monsacré, Pol Hervé, Corentin Werner, Andréas Grosgogeat, Brigitte Blanché, Hélène Rabiega, Pascaline Laupie, Julien Kochert, Fabienne Abraham, Nathalie Sebaoun, Jean-Marc de Lamballerie, Xavier Charrel, Remi Souty, Cecile Camara, Ibrahima Pergeline, Jeanne Noël, Harold Guerrisi, Caroline van der Werf, Sylvie Carrat, Fabrice Hanslik, Thomas Blanchon, Thierry Falchi, Alessandra Pathogens Study Protocol Background: The protocol study will focus on the seroprevalence of IgG antibodies to SARS-CoV-2 achieved by vaccination and/or natural protection as well as the history, symptoms, and risk factors for SARS-CoV-2 in four primary health-care workers (PHCWs) and their household contacts in metropolitan France. Methods: Here, we propose a protocol for a nationwide survey to determine the seroprevalence of IgG antibodies to SARS-CoV-2 achieved by vaccination and/or natural protection in four PHCW populations (general practitioners, pediatricians, pharmacists and assistants, and dentists and assistants) and their household contacts. Participants will be included from June to July 2021 (Phase 1) among PHCW populations located throughout metropolitan France. They will be asked to provide a range of demographic and behavioral information since the first SARS-CoV-2 wave and a self-sampled dried blood spot. Phase 1 will involve also a questionnaire and serological study of PHCWs’ household contacts. Seroprevalence will be estimated using two ELISAs designed to detect specific IgG antibodies to SARS-CoV-2 in humoral fluid, and these results will be confirmed using a virus neutralization test. This study will be repeated from November to December 2021 (Phase 2) to evaluate the evolution of immune status achieved by vaccination and/or natural protection of PHCWs and to describe the history of exposure to SARS-CoV-2. MDPI 2021-07-20 /pmc/articles/PMC8308883/ /pubmed/34358061 http://dx.doi.org/10.3390/pathogens10070911 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Study Protocol
Pouquet, Marie
Decarreaux, Dorine
Prévot-Monsacré, Pol
Hervé, Corentin
Werner, Andréas
Grosgogeat, Brigitte
Blanché, Hélène
Rabiega, Pascaline
Laupie, Julien
Kochert, Fabienne
Abraham, Nathalie
Sebaoun, Jean-Marc
de Lamballerie, Xavier
Charrel, Remi
Souty, Cecile
Camara, Ibrahima
Pergeline, Jeanne
Noël, Harold
Guerrisi, Caroline
van der Werf, Sylvie
Carrat, Fabrice
Hanslik, Thomas
Blanchon, Thierry
Falchi, Alessandra
Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol
title Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol
title_full Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol
title_fullStr Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol
title_full_unstemmed Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol
title_short Nationwide Seroprevalence of SARS-CoV-2 IgG Antibodies among Four Groups of Primary Health-Care Workers and Their Household Contacts 6 Months after the Initiation of the COVID-19 Vaccination Campaign in France: SeroPRIM Study Protocol
title_sort nationwide seroprevalence of sars-cov-2 igg antibodies among four groups of primary health-care workers and their household contacts 6 months after the initiation of the covid-19 vaccination campaign in france: seroprim study protocol
topic Study Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308883/
https://www.ncbi.nlm.nih.gov/pubmed/34358061
http://dx.doi.org/10.3390/pathogens10070911
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