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ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan

The genus Jasminum L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of Jasminum from...

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Autores principales: Akhtar, Naeem, Hafiz, Ishfaq Ahmad, Hayat, Muhammad Qasim, Potter, Daniel, Abbasi, Nadeem Akhtar, Habib, Umer, Hussain, Adil, Hafeez, Hina, Bashir, Muhammad Ajmal, Malik, Saad Imran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308950/
https://www.ncbi.nlm.nih.gov/pubmed/34206638
http://dx.doi.org/10.3390/plants10071270
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author Akhtar, Naeem
Hafiz, Ishfaq Ahmad
Hayat, Muhammad Qasim
Potter, Daniel
Abbasi, Nadeem Akhtar
Habib, Umer
Hussain, Adil
Hafeez, Hina
Bashir, Muhammad Ajmal
Malik, Saad Imran
author_facet Akhtar, Naeem
Hafiz, Ishfaq Ahmad
Hayat, Muhammad Qasim
Potter, Daniel
Abbasi, Nadeem Akhtar
Habib, Umer
Hussain, Adil
Hafeez, Hina
Bashir, Muhammad Ajmal
Malik, Saad Imran
author_sort Akhtar, Naeem
collection PubMed
description The genus Jasminum L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of Jasminum from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon’s information index, polymorphism percentage, effective allele number, Nei’s genetic variations, and genetic unbiased diversity were found in Jasminum sambac L. and J. humile L., while the lowest were observed in J. mesnyi Hance and J. nitidum Skan. Based on Nei’s unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between J. elongatum Willd and J. multiflorum (Burm. f.) Andrews, and the lowest (0.566) between J. nitidum Skan. and J. azoricum L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in Jasminum species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the Jasminum species.
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spelling pubmed-83089502021-07-25 ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan Akhtar, Naeem Hafiz, Ishfaq Ahmad Hayat, Muhammad Qasim Potter, Daniel Abbasi, Nadeem Akhtar Habib, Umer Hussain, Adil Hafeez, Hina Bashir, Muhammad Ajmal Malik, Saad Imran Plants (Basel) Article The genus Jasminum L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of Jasminum from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon’s information index, polymorphism percentage, effective allele number, Nei’s genetic variations, and genetic unbiased diversity were found in Jasminum sambac L. and J. humile L., while the lowest were observed in J. mesnyi Hance and J. nitidum Skan. Based on Nei’s unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between J. elongatum Willd and J. multiflorum (Burm. f.) Andrews, and the lowest (0.566) between J. nitidum Skan. and J. azoricum L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in Jasminum species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the Jasminum species. MDPI 2021-06-22 /pmc/articles/PMC8308950/ /pubmed/34206638 http://dx.doi.org/10.3390/plants10071270 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akhtar, Naeem
Hafiz, Ishfaq Ahmad
Hayat, Muhammad Qasim
Potter, Daniel
Abbasi, Nadeem Akhtar
Habib, Umer
Hussain, Adil
Hafeez, Hina
Bashir, Muhammad Ajmal
Malik, Saad Imran
ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan
title ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan
title_full ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan
title_fullStr ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan
title_full_unstemmed ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan
title_short ISSR-Based Genetic Diversity Assessment of Genus Jasminum L. (Oleaceae) from Pakistan
title_sort issr-based genetic diversity assessment of genus jasminum l. (oleaceae) from pakistan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308950/
https://www.ncbi.nlm.nih.gov/pubmed/34206638
http://dx.doi.org/10.3390/plants10071270
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