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Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio
Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308985/ https://www.ncbi.nlm.nih.gov/pubmed/34371692 http://dx.doi.org/10.3390/pharmaceutics13071000 |
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author | Cruz-Ramírez, Omar Ulises Valenzuela-Salas, Lucía Margarita Blanco-Salazar, Alberto Rodríguez-Arenas, José Antonio Mier-Maldonado, Paris A. García-Ramos, Juan Carlos Bogdanchikova, Nina Pestryakov, Alexey Toledano-Magaña, Yanis |
author_facet | Cruz-Ramírez, Omar Ulises Valenzuela-Salas, Lucía Margarita Blanco-Salazar, Alberto Rodríguez-Arenas, José Antonio Mier-Maldonado, Paris A. García-Ramos, Juan Carlos Bogdanchikova, Nina Pestryakov, Alexey Toledano-Magaña, Yanis |
author_sort | Cruz-Ramírez, Omar Ulises |
collection | PubMed |
description | Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14–1.32% w/w, PVP: 19.6–24.5% and H(2)O: 74.2–79.2% with predominant spherical shape within an average size range of 16–30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5–10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations. |
format | Online Article Text |
id | pubmed-8308985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83089852021-07-25 Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio Cruz-Ramírez, Omar Ulises Valenzuela-Salas, Lucía Margarita Blanco-Salazar, Alberto Rodríguez-Arenas, José Antonio Mier-Maldonado, Paris A. García-Ramos, Juan Carlos Bogdanchikova, Nina Pestryakov, Alexey Toledano-Magaña, Yanis Pharmaceutics Article Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14–1.32% w/w, PVP: 19.6–24.5% and H(2)O: 74.2–79.2% with predominant spherical shape within an average size range of 16–30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5–10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations. MDPI 2021-07-01 /pmc/articles/PMC8308985/ /pubmed/34371692 http://dx.doi.org/10.3390/pharmaceutics13071000 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cruz-Ramírez, Omar Ulises Valenzuela-Salas, Lucía Margarita Blanco-Salazar, Alberto Rodríguez-Arenas, José Antonio Mier-Maldonado, Paris A. García-Ramos, Juan Carlos Bogdanchikova, Nina Pestryakov, Alexey Toledano-Magaña, Yanis Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
title | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
title_full | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
title_fullStr | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
title_full_unstemmed | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
title_short | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
title_sort | antitumor activity against human colorectal adenocarcinoma of silver nanoparticles: influence of [ag]/[pvp] ratio |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308985/ https://www.ncbi.nlm.nih.gov/pubmed/34371692 http://dx.doi.org/10.3390/pharmaceutics13071000 |
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