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Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies
This study was conducted to develop a rapid, simple and reproducible method for the quantification of ceftaroline in plasma samples by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Sample processing consisted of methanol precipitation and then, after centrifugation, th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309110/ https://www.ncbi.nlm.nih.gov/pubmed/34202113 http://dx.doi.org/10.3390/pharmaceutics13070959 |
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author | Alarcia-Lacalle, Ana Barrasa, Helena Maynar, Javier Canut-Blasco, Andrés Gómez-González, Carmen Solinís, María Ángeles Isla, Arantxazu Rodríguez-Gascón, Alicia |
author_facet | Alarcia-Lacalle, Ana Barrasa, Helena Maynar, Javier Canut-Blasco, Andrés Gómez-González, Carmen Solinís, María Ángeles Isla, Arantxazu Rodríguez-Gascón, Alicia |
author_sort | Alarcia-Lacalle, Ana |
collection | PubMed |
description | This study was conducted to develop a rapid, simple and reproducible method for the quantification of ceftaroline in plasma samples by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Sample processing consisted of methanol precipitation and then, after centrifugation, the supernatant was injected into the HPLC system, working in isocratic mode. Ceftaroline was detected at 238 nm at a short acquisition time (less than 5 min). The calibration curve was linear over the concentration range from 0.25 to 40 µg/mL, and the method appeared to be selective, precise and accurate. Ceftaroline in plasma samples was stable at −80 °C for at least 3 months. The method was successfully applied to characterize the pharmacokinetic profile of ceftaroline in two critically ill patients and to evaluate whether the pharmacokinetic/pharmacodynamic (PK/PD) target was reached or not with the dose regimen administered. |
format | Online Article Text |
id | pubmed-8309110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83091102021-07-25 Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies Alarcia-Lacalle, Ana Barrasa, Helena Maynar, Javier Canut-Blasco, Andrés Gómez-González, Carmen Solinís, María Ángeles Isla, Arantxazu Rodríguez-Gascón, Alicia Pharmaceutics Article This study was conducted to develop a rapid, simple and reproducible method for the quantification of ceftaroline in plasma samples by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Sample processing consisted of methanol precipitation and then, after centrifugation, the supernatant was injected into the HPLC system, working in isocratic mode. Ceftaroline was detected at 238 nm at a short acquisition time (less than 5 min). The calibration curve was linear over the concentration range from 0.25 to 40 µg/mL, and the method appeared to be selective, precise and accurate. Ceftaroline in plasma samples was stable at −80 °C for at least 3 months. The method was successfully applied to characterize the pharmacokinetic profile of ceftaroline in two critically ill patients and to evaluate whether the pharmacokinetic/pharmacodynamic (PK/PD) target was reached or not with the dose regimen administered. MDPI 2021-06-25 /pmc/articles/PMC8309110/ /pubmed/34202113 http://dx.doi.org/10.3390/pharmaceutics13070959 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alarcia-Lacalle, Ana Barrasa, Helena Maynar, Javier Canut-Blasco, Andrés Gómez-González, Carmen Solinís, María Ángeles Isla, Arantxazu Rodríguez-Gascón, Alicia Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies |
title | Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies |
title_full | Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies |
title_fullStr | Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies |
title_full_unstemmed | Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies |
title_short | Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies |
title_sort | quantification of ceftaroline in human plasma using high-performance liquid chromatography with ultraviolet detection: application to pharmacokinetic studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309110/ https://www.ncbi.nlm.nih.gov/pubmed/34202113 http://dx.doi.org/10.3390/pharmaceutics13070959 |
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