Cargando…

Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies

Iodine, being an intrinsic part of thyroid hormones, is a vital microelement required for normal growth and development, particularly in children. Inadequate daily intake of iodine causes iodine deficiency, which is responsible for several health disorders, such as cretinism and goiters. Therefore,...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharipov, Avez, Boboev, Zufar, Fazliev, Sunnatullo, Gulyamov, Shokhid, Yunuskhodjayev, Akhmatkhodja, Razzokov, Jamoliddin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309194/
https://www.ncbi.nlm.nih.gov/pubmed/34201915
http://dx.doi.org/10.3390/pharmaceutics13070955
_version_ 1783728465143398400
author Sharipov, Avez
Boboev, Zufar
Fazliev, Sunnatullo
Gulyamov, Shokhid
Yunuskhodjayev, Akhmatkhodja
Razzokov, Jamoliddin
author_facet Sharipov, Avez
Boboev, Zufar
Fazliev, Sunnatullo
Gulyamov, Shokhid
Yunuskhodjayev, Akhmatkhodja
Razzokov, Jamoliddin
author_sort Sharipov, Avez
collection PubMed
description Iodine, being an intrinsic part of thyroid hormones, is a vital microelement required for normal growth and development, particularly in children. Inadequate daily intake of iodine causes iodine deficiency, which is responsible for several health disorders, such as cretinism and goiters. Therefore, the development of new drugs and/or food supplements for iodine deficiency is crucial. We synthesized an iodine/β-cyclodextrin complex based on a host–guest model, and in this paper, we outline the development of a new quantitative analysis method. We suggest a robust and reliable high-performance liquid chromatography method to determine the total amount of iodine species in the complex. Moreover, we performed validation of our method. The results of validation presented here show the reliability, accuracy and high precision of the method. Furthermore, for the first time, we show results of in vivo studies for the thyroid-stimulating activity of the iodine/β-cyclodextrin complex. Our findings indicate that the thyroid-stimulating activity of iodine/β-cyclodextrin is comparable to that of potassium iodide, which is the main active pharmaceutical substance of conventional drugs for iodine deficiency.
format Online
Article
Text
id pubmed-8309194
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83091942021-07-25 Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies Sharipov, Avez Boboev, Zufar Fazliev, Sunnatullo Gulyamov, Shokhid Yunuskhodjayev, Akhmatkhodja Razzokov, Jamoliddin Pharmaceutics Article Iodine, being an intrinsic part of thyroid hormones, is a vital microelement required for normal growth and development, particularly in children. Inadequate daily intake of iodine causes iodine deficiency, which is responsible for several health disorders, such as cretinism and goiters. Therefore, the development of new drugs and/or food supplements for iodine deficiency is crucial. We synthesized an iodine/β-cyclodextrin complex based on a host–guest model, and in this paper, we outline the development of a new quantitative analysis method. We suggest a robust and reliable high-performance liquid chromatography method to determine the total amount of iodine species in the complex. Moreover, we performed validation of our method. The results of validation presented here show the reliability, accuracy and high precision of the method. Furthermore, for the first time, we show results of in vivo studies for the thyroid-stimulating activity of the iodine/β-cyclodextrin complex. Our findings indicate that the thyroid-stimulating activity of iodine/β-cyclodextrin is comparable to that of potassium iodide, which is the main active pharmaceutical substance of conventional drugs for iodine deficiency. MDPI 2021-06-25 /pmc/articles/PMC8309194/ /pubmed/34201915 http://dx.doi.org/10.3390/pharmaceutics13070955 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharipov, Avez
Boboev, Zufar
Fazliev, Sunnatullo
Gulyamov, Shokhid
Yunuskhodjayev, Akhmatkhodja
Razzokov, Jamoliddin
Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies
title Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies
title_full Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies
title_fullStr Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies
title_full_unstemmed Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies
title_short Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies
title_sort development of an improved method for the determination of iodine/β-cyclodextrin by means of hplc-uv: validation and the thyroid-stimulating activity revealed by in vivo studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309194/
https://www.ncbi.nlm.nih.gov/pubmed/34201915
http://dx.doi.org/10.3390/pharmaceutics13070955
work_keys_str_mv AT sharipovavez developmentofanimprovedmethodforthedeterminationofiodinebcyclodextrinbymeansofhplcuvvalidationandthethyroidstimulatingactivityrevealedbyinvivostudies
AT boboevzufar developmentofanimprovedmethodforthedeterminationofiodinebcyclodextrinbymeansofhplcuvvalidationandthethyroidstimulatingactivityrevealedbyinvivostudies
AT fazlievsunnatullo developmentofanimprovedmethodforthedeterminationofiodinebcyclodextrinbymeansofhplcuvvalidationandthethyroidstimulatingactivityrevealedbyinvivostudies
AT gulyamovshokhid developmentofanimprovedmethodforthedeterminationofiodinebcyclodextrinbymeansofhplcuvvalidationandthethyroidstimulatingactivityrevealedbyinvivostudies
AT yunuskhodjayevakhmatkhodja developmentofanimprovedmethodforthedeterminationofiodinebcyclodextrinbymeansofhplcuvvalidationandthethyroidstimulatingactivityrevealedbyinvivostudies
AT razzokovjamoliddin developmentofanimprovedmethodforthedeterminationofiodinebcyclodextrinbymeansofhplcuvvalidationandthethyroidstimulatingactivityrevealedbyinvivostudies