Stomatal Traits and Barley (Hordeum vulgare L.) Forage Yield in Drought Conditions of Northeastern Mexico

Infrared technology is a practical, fast, non-destructive method that helps in forecasting plant development and can be used to select physiological traits, instead of other methodologies that require more time and breeding efforts. According to the statistical analyses and the relationship between the direct and indirect effects of the variables, this technology could serve as the basis for implementing a genotype selection methodology. Awnless barley was assessed in a randomized block design with three replicates in two crop seasons at Mexico’s northeastern region. Two samplings were carried out during crop development: at 75 and 90 days after sowing. The infrared and stomatal sensors were used to identify the direct and indirect effects of stomata’s traits on dry forage yield. The data were analyzed in a subdivided plot design, using mean comparison tests, correlation coefficients and path analyses, finding significant differences (p < 0.05) among localities and among samplings. Dry forage yield was significant and positively correlated with plant height (r = 0.578) and canopy temperature (r = 0.724), and negatively correlated with the leaf upper side stomatal width (r = −0.409) and the leaf lower side stomatal width (r = −0.641), chlorophyll content and vegetation index. Temperature, chlorophyll, density and leaf lower side stomatal index had the strongest direct effects on yield. Therefore, the infrared technology appears as a way to select high yielding awnless forage barley, to obtain the correlation, the positive direct effect of temperature and the negative effect of chlorophyll. Due to their direct effects, low density and low leaf underside stomatal indexes can also help in the indirect selection of higher yielding forage barley genotypes.