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Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell

Gravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of Eug...

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Autores principales: Ozasa, Kazunari, Kang, Hyunwoong, Song, Simon, Kato, Shota, Shinomura, Tomoko, Maeda, Mizuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309284/
https://www.ncbi.nlm.nih.gov/pubmed/34371614
http://dx.doi.org/10.3390/plants10071411
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author Ozasa, Kazunari
Kang, Hyunwoong
Song, Simon
Kato, Shota
Shinomura, Tomoko
Maeda, Mizuo
author_facet Ozasa, Kazunari
Kang, Hyunwoong
Song, Simon
Kato, Shota
Shinomura, Tomoko
Maeda, Mizuo
author_sort Ozasa, Kazunari
collection PubMed
description Gravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of Euglena gracilis using vertically placed microchambers containing a single cell. A temporal change in gravitaxis and cell multiplication was observed after transferring the cells to fresh culture medium for 9 days. We performed 29 individual experiments with 2.5 mm × 2.5 mm × 0.1 mm square microchambers and found that the cells showed positive, negative, and moderate gravitaxis in 8, 7, and 14 cases, respectively, after transferring to fresh culture medium. A common trend was observed for the temporal change in gravitaxis for the eight initially positive gravitaxis cases. The cells with initially positive gravitaxis showed a higher rate of cell multiplication than those with initially negative gravitaxis. We also discussed the gravitaxis mechanism of E. gracilis from the observed trend of gravitaxis change and swimming traces. In addition, bioconvection in a larger and thicker chamber was investigated at a millimeter scale and visualized.
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spelling pubmed-83092842021-07-25 Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell Ozasa, Kazunari Kang, Hyunwoong Song, Simon Kato, Shota Shinomura, Tomoko Maeda, Mizuo Plants (Basel) Article Gravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of Euglena gracilis using vertically placed microchambers containing a single cell. A temporal change in gravitaxis and cell multiplication was observed after transferring the cells to fresh culture medium for 9 days. We performed 29 individual experiments with 2.5 mm × 2.5 mm × 0.1 mm square microchambers and found that the cells showed positive, negative, and moderate gravitaxis in 8, 7, and 14 cases, respectively, after transferring to fresh culture medium. A common trend was observed for the temporal change in gravitaxis for the eight initially positive gravitaxis cases. The cells with initially positive gravitaxis showed a higher rate of cell multiplication than those with initially negative gravitaxis. We also discussed the gravitaxis mechanism of E. gracilis from the observed trend of gravitaxis change and swimming traces. In addition, bioconvection in a larger and thicker chamber was investigated at a millimeter scale and visualized. MDPI 2021-07-09 /pmc/articles/PMC8309284/ /pubmed/34371614 http://dx.doi.org/10.3390/plants10071411 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ozasa, Kazunari
Kang, Hyunwoong
Song, Simon
Kato, Shota
Shinomura, Tomoko
Maeda, Mizuo
Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
title Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
title_full Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
title_fullStr Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
title_full_unstemmed Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
title_short Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
title_sort temporal evolution of the gravitaxis of euglena gracilis from a single cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309284/
https://www.ncbi.nlm.nih.gov/pubmed/34371614
http://dx.doi.org/10.3390/plants10071411
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