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Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection

Wheat is a major nutritional cereal crop that has economic and strategic value worldwide. The sustainability of this extraordinary crop is facing critical challenges globally, particularly leaf rust disease, which causes endless problems for wheat farmers and countries and negatively affects humanit...

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Autores principales: Atia, Mohamed A. M., El-Khateeb, Eman A., Abd El-Maksoud, Reem M., Abou-Zeid, Mohamed A., Salah, Arwa, Abdel-Hamid, Amal M. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309345/
https://www.ncbi.nlm.nih.gov/pubmed/34371581
http://dx.doi.org/10.3390/plants10071378
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author Atia, Mohamed A. M.
El-Khateeb, Eman A.
Abd El-Maksoud, Reem M.
Abou-Zeid, Mohamed A.
Salah, Arwa
Abdel-Hamid, Amal M. E.
author_facet Atia, Mohamed A. M.
El-Khateeb, Eman A.
Abd El-Maksoud, Reem M.
Abou-Zeid, Mohamed A.
Salah, Arwa
Abdel-Hamid, Amal M. E.
author_sort Atia, Mohamed A. M.
collection PubMed
description Wheat is a major nutritional cereal crop that has economic and strategic value worldwide. The sustainability of this extraordinary crop is facing critical challenges globally, particularly leaf rust disease, which causes endless problems for wheat farmers and countries and negatively affects humanity’s food security. Developing effective marker-assisted selection programs for leaf rust resistance in wheat mainly depends on the availability of deep mining of resistance genes within the germplasm collections. This is the first study that evaluated the leaf rust resistance of 50 Egyptian wheat varieties at the adult plant stage for two successive seasons and identified the absence/presence of 28 leaf rust resistance (Lr) genes within the studied wheat collection. The field evaluation results indicated that most of these varieties demonstrated high to moderate leaf rust resistance levels except Gemmeiza 1, Gemmeiza 9, Giza162, Giza 163, Giza 164, Giza 165, Sids 1, Sids 2, Sids 3, Sakha 62, Sakha 69, Sohag 3 and Bany Swif 4, which showed fast rusting behavior. On the other hand, out of these 28 Lr genes tested against the wheat collection, 21 Lr genes were successfully identified. Out of 15 Lr genes reported conferring the adult plant resistant or slow rusting behavior in wheat, only five genes (Lr13, Lr22a, Lr34, Lr37, and Lr67) were detected within the Egyptian collection. Remarkedly, the genes Lr13, Lr19, Lr20, Lr22a, Lr28, Lr29, Lr32, Lr34, Lr36, Lr47, and Lr60, were found to be the most predominant Lr genes across the 50 Egyptian wheat varieties. The molecular phylogeny results also inferred the same classification of field evaluation, through grouping genotypes characterized by high to moderate leaf rust resistance in one cluster while being highly susceptible in a separate cluster, with few exceptions.
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spelling pubmed-83093452021-07-25 Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection Atia, Mohamed A. M. El-Khateeb, Eman A. Abd El-Maksoud, Reem M. Abou-Zeid, Mohamed A. Salah, Arwa Abdel-Hamid, Amal M. E. Plants (Basel) Article Wheat is a major nutritional cereal crop that has economic and strategic value worldwide. The sustainability of this extraordinary crop is facing critical challenges globally, particularly leaf rust disease, which causes endless problems for wheat farmers and countries and negatively affects humanity’s food security. Developing effective marker-assisted selection programs for leaf rust resistance in wheat mainly depends on the availability of deep mining of resistance genes within the germplasm collections. This is the first study that evaluated the leaf rust resistance of 50 Egyptian wheat varieties at the adult plant stage for two successive seasons and identified the absence/presence of 28 leaf rust resistance (Lr) genes within the studied wheat collection. The field evaluation results indicated that most of these varieties demonstrated high to moderate leaf rust resistance levels except Gemmeiza 1, Gemmeiza 9, Giza162, Giza 163, Giza 164, Giza 165, Sids 1, Sids 2, Sids 3, Sakha 62, Sakha 69, Sohag 3 and Bany Swif 4, which showed fast rusting behavior. On the other hand, out of these 28 Lr genes tested against the wheat collection, 21 Lr genes were successfully identified. Out of 15 Lr genes reported conferring the adult plant resistant or slow rusting behavior in wheat, only five genes (Lr13, Lr22a, Lr34, Lr37, and Lr67) were detected within the Egyptian collection. Remarkedly, the genes Lr13, Lr19, Lr20, Lr22a, Lr28, Lr29, Lr32, Lr34, Lr36, Lr47, and Lr60, were found to be the most predominant Lr genes across the 50 Egyptian wheat varieties. The molecular phylogeny results also inferred the same classification of field evaluation, through grouping genotypes characterized by high to moderate leaf rust resistance in one cluster while being highly susceptible in a separate cluster, with few exceptions. MDPI 2021-07-05 /pmc/articles/PMC8309345/ /pubmed/34371581 http://dx.doi.org/10.3390/plants10071378 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Atia, Mohamed A. M.
El-Khateeb, Eman A.
Abd El-Maksoud, Reem M.
Abou-Zeid, Mohamed A.
Salah, Arwa
Abdel-Hamid, Amal M. E.
Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection
title Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection
title_full Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection
title_fullStr Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection
title_full_unstemmed Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection
title_short Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection
title_sort mining of leaf rust resistance genes content in egyptian bread wheat collection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309345/
https://www.ncbi.nlm.nih.gov/pubmed/34371581
http://dx.doi.org/10.3390/plants10071378
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