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DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells

The chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications an...

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Autores principales: Bara, Adrianna Wiktoria, Braszewska, Agnieszka, Kwasniewska, Jolanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309452/
https://www.ncbi.nlm.nih.gov/pubmed/34371611
http://dx.doi.org/10.3390/plants10071408
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author Bara, Adrianna Wiktoria
Braszewska, Agnieszka
Kwasniewska, Jolanta
author_facet Bara, Adrianna Wiktoria
Braszewska, Agnieszka
Kwasniewska, Jolanta
author_sort Bara, Adrianna Wiktoria
collection PubMed
description The chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications and the instability of the Brachypodium distachyon genome, which are observed as micronuclei, following maleic hydrazide (MH) and nitroso-N-methylurea (MNU) treatments. We compared the level of DNA methylation in the control (untreated) and mutagen-treated B. distachyon nuclei. An immunostaining method using specific antibodies against modified DNA anti-5-methylcytosine was used for the evaluation of DNA methylation in a single nucleus and micronucleus. Interestingly, we showed an alteration of DNA methylation in cells after mutagenic treatments. The results indicate that DNA methylation might be involved in the response of the B. distachyon genome to mutagenic treatments. This demonstrates that analyses of the epigenetic modifications should be integrated into current plant genetic toxicology in order to explain the mechanisms of DNA damage and repair in plants.
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spelling pubmed-83094522021-07-25 DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells Bara, Adrianna Wiktoria Braszewska, Agnieszka Kwasniewska, Jolanta Plants (Basel) Article The chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications and the instability of the Brachypodium distachyon genome, which are observed as micronuclei, following maleic hydrazide (MH) and nitroso-N-methylurea (MNU) treatments. We compared the level of DNA methylation in the control (untreated) and mutagen-treated B. distachyon nuclei. An immunostaining method using specific antibodies against modified DNA anti-5-methylcytosine was used for the evaluation of DNA methylation in a single nucleus and micronucleus. Interestingly, we showed an alteration of DNA methylation in cells after mutagenic treatments. The results indicate that DNA methylation might be involved in the response of the B. distachyon genome to mutagenic treatments. This demonstrates that analyses of the epigenetic modifications should be integrated into current plant genetic toxicology in order to explain the mechanisms of DNA damage and repair in plants. MDPI 2021-07-09 /pmc/articles/PMC8309452/ /pubmed/34371611 http://dx.doi.org/10.3390/plants10071408 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bara, Adrianna Wiktoria
Braszewska, Agnieszka
Kwasniewska, Jolanta
DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
title DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
title_full DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
title_fullStr DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
title_full_unstemmed DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
title_short DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
title_sort dna methylation—an epigenetic mark in mutagen-treated brachypodium distachyon cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309452/
https://www.ncbi.nlm.nih.gov/pubmed/34371611
http://dx.doi.org/10.3390/plants10071408
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