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Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection

Prosthetic mesh infection is a devastating complication of abdominal hernia repair which impairs natural healing in the implant area, leading to increased rates of patient morbidity, mortality, and prolonged hospitalization. This preclinical study was designed to assess the effects on abdominal wall...

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Autores principales: Benito-Martínez, Selma, Pérez-Köhler, Bárbara, Rodríguez, Marta, García-Moreno, Francisca, Gómez-Gil, Verónica, Pascual, Gemma, Bellón, Juan Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309493/
https://www.ncbi.nlm.nih.gov/pubmed/34301128
http://dx.doi.org/10.3390/polym13142371
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author Benito-Martínez, Selma
Pérez-Köhler, Bárbara
Rodríguez, Marta
García-Moreno, Francisca
Gómez-Gil, Verónica
Pascual, Gemma
Bellón, Juan Manuel
author_facet Benito-Martínez, Selma
Pérez-Köhler, Bárbara
Rodríguez, Marta
García-Moreno, Francisca
Gómez-Gil, Verónica
Pascual, Gemma
Bellón, Juan Manuel
author_sort Benito-Martínez, Selma
collection PubMed
description Prosthetic mesh infection is a devastating complication of abdominal hernia repair which impairs natural healing in the implant area, leading to increased rates of patient morbidity, mortality, and prolonged hospitalization. This preclinical study was designed to assess the effects on abdominal wall tissue repair of coating meshes with a chlorhexidine or rifampicin-carboxymethylcellulose biopolymer gel in a Staphylococcus aureus (S. aureus) infection model. Partial abdominal wall defects were created in New Zealand white rabbits (n = 20). Four study groups were established according to whether the meshes were coated or not with each of the antibacterial gels. Three groups were inoculated with S. aureus and finally repaired with lightweight polypropylene mesh. Fourteen days after surgery, implanted meshes were recovered for analysis of the gene and protein expression of collagens, macrophage phenotypes, and mRNA expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Compared to uncoated meshes, those coated with either biopolymer gel showed higher collagen 1/3 messenger RNA and collagen I protein expression, relatively increased VEGF mRNA expression, a significantly reduced macrophage response, and lower relative amounts of MMPs mRNAs. Our findings suggest that following mesh implant these coatings may help improving abdominal wall tissue repair in the presence of infection.
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spelling pubmed-83094932021-07-25 Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection Benito-Martínez, Selma Pérez-Köhler, Bárbara Rodríguez, Marta García-Moreno, Francisca Gómez-Gil, Verónica Pascual, Gemma Bellón, Juan Manuel Polymers (Basel) Article Prosthetic mesh infection is a devastating complication of abdominal hernia repair which impairs natural healing in the implant area, leading to increased rates of patient morbidity, mortality, and prolonged hospitalization. This preclinical study was designed to assess the effects on abdominal wall tissue repair of coating meshes with a chlorhexidine or rifampicin-carboxymethylcellulose biopolymer gel in a Staphylococcus aureus (S. aureus) infection model. Partial abdominal wall defects were created in New Zealand white rabbits (n = 20). Four study groups were established according to whether the meshes were coated or not with each of the antibacterial gels. Three groups were inoculated with S. aureus and finally repaired with lightweight polypropylene mesh. Fourteen days after surgery, implanted meshes were recovered for analysis of the gene and protein expression of collagens, macrophage phenotypes, and mRNA expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Compared to uncoated meshes, those coated with either biopolymer gel showed higher collagen 1/3 messenger RNA and collagen I protein expression, relatively increased VEGF mRNA expression, a significantly reduced macrophage response, and lower relative amounts of MMPs mRNAs. Our findings suggest that following mesh implant these coatings may help improving abdominal wall tissue repair in the presence of infection. MDPI 2021-07-20 /pmc/articles/PMC8309493/ /pubmed/34301128 http://dx.doi.org/10.3390/polym13142371 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Benito-Martínez, Selma
Pérez-Köhler, Bárbara
Rodríguez, Marta
García-Moreno, Francisca
Gómez-Gil, Verónica
Pascual, Gemma
Bellón, Juan Manuel
Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection
title Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection
title_full Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection
title_fullStr Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection
title_full_unstemmed Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection
title_short Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection
title_sort antibacterial biopolymer gel coating on meshes used for abdominal hernia repair promotes effective wound repair in the presence of infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309493/
https://www.ncbi.nlm.nih.gov/pubmed/34301128
http://dx.doi.org/10.3390/polym13142371
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