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DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples
Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular v...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309968/ https://www.ncbi.nlm.nih.gov/pubmed/34372516 http://dx.doi.org/10.3390/v13071310 |
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author | Izumi, Takuma Morioka, Yuhei Urayama, Syun-ichi Motooka, Daisuke Tamura, Tomokazu Kawagishi, Takahiro Kanai, Yuta Kobayashi, Takeshi Ono, Chikako Morinaga, Akinari Tomiyama, Takahiro Iseda, Norifumi Kosai, Yukiko Inokuchi, Shoichi Nakamura, Shota Tanaka, Tomohisa Moriishi, Kohji Kariwa, Hiroaki Yoshizumi, Tomoharu Mori, Masaki Matsuura, Yoshiharu Fukuhara, Takasuke |
author_facet | Izumi, Takuma Morioka, Yuhei Urayama, Syun-ichi Motooka, Daisuke Tamura, Tomokazu Kawagishi, Takahiro Kanai, Yuta Kobayashi, Takeshi Ono, Chikako Morinaga, Akinari Tomiyama, Takahiro Iseda, Norifumi Kosai, Yukiko Inokuchi, Shoichi Nakamura, Shota Tanaka, Tomohisa Moriishi, Kohji Kariwa, Hiroaki Yoshizumi, Tomoharu Mori, Masaki Matsuura, Yoshiharu Fukuhara, Takasuke |
author_sort | Izumi, Takuma |
collection | PubMed |
description | Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular viral genome. However, an efficient method for the detection of a viral genome derived from an RNA virus in animal or human samples has not been established. Here, we established a method for the efficient detection of RNA viruses in human clinical samples. We then tested the efficiency of the method compared to other conventional methods by using tissue samples collected from 57 recipients of living donor liver transplantations performed between June 2017 and February 2019 at Kyushu University Hospital. The viral read ratio in human clinical samples was higher by the new method than by the other conventional methods. In addition, the new method correctly identified viral RNA from liver tissues infected with hepatitis C virus. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases. |
format | Online Article Text |
id | pubmed-8309968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83099682021-07-25 DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples Izumi, Takuma Morioka, Yuhei Urayama, Syun-ichi Motooka, Daisuke Tamura, Tomokazu Kawagishi, Takahiro Kanai, Yuta Kobayashi, Takeshi Ono, Chikako Morinaga, Akinari Tomiyama, Takahiro Iseda, Norifumi Kosai, Yukiko Inokuchi, Shoichi Nakamura, Shota Tanaka, Tomohisa Moriishi, Kohji Kariwa, Hiroaki Yoshizumi, Tomoharu Mori, Masaki Matsuura, Yoshiharu Fukuhara, Takasuke Viruses Article Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular viral genome. However, an efficient method for the detection of a viral genome derived from an RNA virus in animal or human samples has not been established. Here, we established a method for the efficient detection of RNA viruses in human clinical samples. We then tested the efficiency of the method compared to other conventional methods by using tissue samples collected from 57 recipients of living donor liver transplantations performed between June 2017 and February 2019 at Kyushu University Hospital. The viral read ratio in human clinical samples was higher by the new method than by the other conventional methods. In addition, the new method correctly identified viral RNA from liver tissues infected with hepatitis C virus. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases. MDPI 2021-07-06 /pmc/articles/PMC8309968/ /pubmed/34372516 http://dx.doi.org/10.3390/v13071310 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Izumi, Takuma Morioka, Yuhei Urayama, Syun-ichi Motooka, Daisuke Tamura, Tomokazu Kawagishi, Takahiro Kanai, Yuta Kobayashi, Takeshi Ono, Chikako Morinaga, Akinari Tomiyama, Takahiro Iseda, Norifumi Kosai, Yukiko Inokuchi, Shoichi Nakamura, Shota Tanaka, Tomohisa Moriishi, Kohji Kariwa, Hiroaki Yoshizumi, Tomoharu Mori, Masaki Matsuura, Yoshiharu Fukuhara, Takasuke DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
title | DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
title_full | DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
title_fullStr | DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
title_full_unstemmed | DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
title_short | DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples |
title_sort | dsrna sequencing for rna virus surveillance using human clinical samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309968/ https://www.ncbi.nlm.nih.gov/pubmed/34372516 http://dx.doi.org/10.3390/v13071310 |
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