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Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients

COVID-19 is a current global threat, and the characterization of antibody response is vitally important to update vaccine development and strategies. In this study we assessed SARS-CoV-2 antibody concentrations in SARS-CoV-2 positive patients (N = 272) and subjects vaccinated with the BNT162b2 m-RNA...

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Autores principales: Amodio, Emanuele, Capra, Giuseppina, Casuccio, Alessandra, Grazia, Simona De, Genovese, Dario, Pizzo, Stefano, Calamusa, Giuseppe, Ferraro, Donatella, Giammanco, Giovanni Maurizio, Vitale, Francesco, Bonura, Floriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309986/
https://www.ncbi.nlm.nih.gov/pubmed/34358130
http://dx.doi.org/10.3390/vaccines9070714
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author Amodio, Emanuele
Capra, Giuseppina
Casuccio, Alessandra
Grazia, Simona De
Genovese, Dario
Pizzo, Stefano
Calamusa, Giuseppe
Ferraro, Donatella
Giammanco, Giovanni Maurizio
Vitale, Francesco
Bonura, Floriana
author_facet Amodio, Emanuele
Capra, Giuseppina
Casuccio, Alessandra
Grazia, Simona De
Genovese, Dario
Pizzo, Stefano
Calamusa, Giuseppe
Ferraro, Donatella
Giammanco, Giovanni Maurizio
Vitale, Francesco
Bonura, Floriana
author_sort Amodio, Emanuele
collection PubMed
description COVID-19 is a current global threat, and the characterization of antibody response is vitally important to update vaccine development and strategies. In this study we assessed SARS-CoV-2 antibody concentrations in SARS-CoV-2 positive patients (N = 272) and subjects vaccinated with the BNT162b2 m-RNA COVID-19 vaccine (N = 1256). For each participant, socio-demographic data, COVID-19 vaccination records, serological analyses, and SARS-CoV-2 infection status were collected. IgG antibodies against S1/S2 antigens of SARS-CoV-2 were detected. Almost all vaccinated subjects (99.8%) showed a seropositivity to anti-SARS-COV-2 IgG and more than 80% of vaccinated subjects had IgG concentrations > 200 AU/mL. In a Tobit multivariable regression analysis, SARS-CoV-2 vaccination was statistically significantly associated with increased IgG concentrations (β coef = 266.4; p < 0.001). A statistically significant reduction in SARS-CoV-2 IgG concentrations was found with older age (β coef = −1.96 per year increase; p < 0.001), male sex (β coef = −22.3; p < 0.001), and days after immunization (β coef = −1.67 per day increase; p < 0.001). Our findings could support the vaccination campaigns confirming the high immunogenicity of the SARS-CoV-2 vaccine under investigation with respect to the natural infection. Further studies will be required for evaluating the role of age and days after immunization in the persistence of vaccine antibodies and protection from the disease.
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spelling pubmed-83099862021-07-25 Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients Amodio, Emanuele Capra, Giuseppina Casuccio, Alessandra Grazia, Simona De Genovese, Dario Pizzo, Stefano Calamusa, Giuseppe Ferraro, Donatella Giammanco, Giovanni Maurizio Vitale, Francesco Bonura, Floriana Vaccines (Basel) Article COVID-19 is a current global threat, and the characterization of antibody response is vitally important to update vaccine development and strategies. In this study we assessed SARS-CoV-2 antibody concentrations in SARS-CoV-2 positive patients (N = 272) and subjects vaccinated with the BNT162b2 m-RNA COVID-19 vaccine (N = 1256). For each participant, socio-demographic data, COVID-19 vaccination records, serological analyses, and SARS-CoV-2 infection status were collected. IgG antibodies against S1/S2 antigens of SARS-CoV-2 were detected. Almost all vaccinated subjects (99.8%) showed a seropositivity to anti-SARS-COV-2 IgG and more than 80% of vaccinated subjects had IgG concentrations > 200 AU/mL. In a Tobit multivariable regression analysis, SARS-CoV-2 vaccination was statistically significantly associated with increased IgG concentrations (β coef = 266.4; p < 0.001). A statistically significant reduction in SARS-CoV-2 IgG concentrations was found with older age (β coef = −1.96 per year increase; p < 0.001), male sex (β coef = −22.3; p < 0.001), and days after immunization (β coef = −1.67 per day increase; p < 0.001). Our findings could support the vaccination campaigns confirming the high immunogenicity of the SARS-CoV-2 vaccine under investigation with respect to the natural infection. Further studies will be required for evaluating the role of age and days after immunization in the persistence of vaccine antibodies and protection from the disease. MDPI 2021-07-01 /pmc/articles/PMC8309986/ /pubmed/34358130 http://dx.doi.org/10.3390/vaccines9070714 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amodio, Emanuele
Capra, Giuseppina
Casuccio, Alessandra
Grazia, Simona De
Genovese, Dario
Pizzo, Stefano
Calamusa, Giuseppe
Ferraro, Donatella
Giammanco, Giovanni Maurizio
Vitale, Francesco
Bonura, Floriana
Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients
title Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients
title_full Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients
title_fullStr Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients
title_full_unstemmed Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients
title_short Antibodies Responses to SARS-CoV-2 in a Large Cohort of Vaccinated Subjects and Seropositive Patients
title_sort antibodies responses to sars-cov-2 in a large cohort of vaccinated subjects and seropositive patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309986/
https://www.ncbi.nlm.nih.gov/pubmed/34358130
http://dx.doi.org/10.3390/vaccines9070714
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