Cargando…

Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene

African swine fever (ASF) is an emerging disease threat to the swine industry worldwide. There is no vaccine against ASF, and progress is hindered by a lack of knowledge concerning the extent of ASFV strain diversity and the viral antigens conferring type-specific protective immunity in pigs. We hav...

Descripción completa

Detalles Bibliográficos
Autores principales: Koltsova, Galina, Koltsov, Andrey, Krutko, Sergey, Kholod, Natalia, Tulman, Edan R., Kolbasov, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309992/
https://www.ncbi.nlm.nih.gov/pubmed/34203302
http://dx.doi.org/10.3390/v13071259
_version_ 1783728653605011456
author Koltsova, Galina
Koltsov, Andrey
Krutko, Sergey
Kholod, Natalia
Tulman, Edan R.
Kolbasov, Denis
author_facet Koltsova, Galina
Koltsov, Andrey
Krutko, Sergey
Kholod, Natalia
Tulman, Edan R.
Kolbasov, Denis
author_sort Koltsova, Galina
collection PubMed
description African swine fever (ASF) is an emerging disease threat to the swine industry worldwide. There is no vaccine against ASF, and progress is hindered by a lack of knowledge concerning the extent of ASFV strain diversity and the viral antigens conferring type-specific protective immunity in pigs. We have previously demonstrated that homologous ASFV serotype-specific proteins CD2v (EP402R) and/or C-type lectin are required for protection against challenge with the virulent ASFV strain Congo (Genotype I, Serogroup 2), and we have identified T-cell epitopes on CD2v which may be associated with serotype-specific protection. Here, using a cell-culture adapted derivative of the ASFV strain Congo (Congo-a) with specific deletion of the EP402R gene (ΔCongoCD2v) in swine vaccination/challenge experiments, we demonstrated that deletion of the EP402R gene results in the failure of ΔCongoCD2v to induce protection against challenge with the virulent strain Congo (Congo-v). While ΔCongoCD2v growth kinetics in COS-1 cells and primary swine macrophage culture were almost identical to parental Congo-a, replication of ΔCongoCD2v in vivo was significantly reduced compared with parental Congo-a. Our data support the idea that the CD2v protein is important for the ability of homologous live-attenuated vaccines to induce protective immunity against the ASFV strain Congo challenge in vivo.
format Online
Article
Text
id pubmed-8309992
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83099922021-07-25 Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene Koltsova, Galina Koltsov, Andrey Krutko, Sergey Kholod, Natalia Tulman, Edan R. Kolbasov, Denis Viruses Article African swine fever (ASF) is an emerging disease threat to the swine industry worldwide. There is no vaccine against ASF, and progress is hindered by a lack of knowledge concerning the extent of ASFV strain diversity and the viral antigens conferring type-specific protective immunity in pigs. We have previously demonstrated that homologous ASFV serotype-specific proteins CD2v (EP402R) and/or C-type lectin are required for protection against challenge with the virulent ASFV strain Congo (Genotype I, Serogroup 2), and we have identified T-cell epitopes on CD2v which may be associated with serotype-specific protection. Here, using a cell-culture adapted derivative of the ASFV strain Congo (Congo-a) with specific deletion of the EP402R gene (ΔCongoCD2v) in swine vaccination/challenge experiments, we demonstrated that deletion of the EP402R gene results in the failure of ΔCongoCD2v to induce protection against challenge with the virulent strain Congo (Congo-v). While ΔCongoCD2v growth kinetics in COS-1 cells and primary swine macrophage culture were almost identical to parental Congo-a, replication of ΔCongoCD2v in vivo was significantly reduced compared with parental Congo-a. Our data support the idea that the CD2v protein is important for the ability of homologous live-attenuated vaccines to induce protective immunity against the ASFV strain Congo challenge in vivo. MDPI 2021-06-28 /pmc/articles/PMC8309992/ /pubmed/34203302 http://dx.doi.org/10.3390/v13071259 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koltsova, Galina
Koltsov, Andrey
Krutko, Sergey
Kholod, Natalia
Tulman, Edan R.
Kolbasov, Denis
Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene
title Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene
title_full Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene
title_fullStr Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene
title_full_unstemmed Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene
title_short Growth Kinetics and Protective Efficacy of Attenuated ASFV Strain Congo with Deletion of the EP402 Gene
title_sort growth kinetics and protective efficacy of attenuated asfv strain congo with deletion of the ep402 gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309992/
https://www.ncbi.nlm.nih.gov/pubmed/34203302
http://dx.doi.org/10.3390/v13071259
work_keys_str_mv AT koltsovagalina growthkineticsandprotectiveefficacyofattenuatedasfvstraincongowithdeletionoftheep402gene
AT koltsovandrey growthkineticsandprotectiveefficacyofattenuatedasfvstraincongowithdeletionoftheep402gene
AT krutkosergey growthkineticsandprotectiveefficacyofattenuatedasfvstraincongowithdeletionoftheep402gene
AT kholodnatalia growthkineticsandprotectiveefficacyofattenuatedasfvstraincongowithdeletionoftheep402gene
AT tulmanedanr growthkineticsandprotectiveefficacyofattenuatedasfvstraincongowithdeletionoftheep402gene
AT kolbasovdenis growthkineticsandprotectiveefficacyofattenuatedasfvstraincongowithdeletionoftheep402gene