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First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies

Bulgaria, situated on the Balkan Peninsula, is rich in small and shallow, natural and man-made non-lotic waterbodies, which are threatened by blooms of Cyanoprokaryota/Cyanobacteria. Although cyanotoxins in Bulgarian surface waters are receiving increased attention, there is no information on microv...

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Autores principales: Uzunov, Blagoy, Stefanova, Katerina, Radkova, Mariana, Descy, Jean-Pierre, Gärtner, Georg, Stoyneva-Gärtner, Maya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310014/
https://www.ncbi.nlm.nih.gov/pubmed/34203459
http://dx.doi.org/10.3390/toxins13070448
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author Uzunov, Blagoy
Stefanova, Katerina
Radkova, Mariana
Descy, Jean-Pierre
Gärtner, Georg
Stoyneva-Gärtner, Maya
author_facet Uzunov, Blagoy
Stefanova, Katerina
Radkova, Mariana
Descy, Jean-Pierre
Gärtner, Georg
Stoyneva-Gärtner, Maya
author_sort Uzunov, Blagoy
collection PubMed
description Bulgaria, situated on the Balkan Peninsula, is rich in small and shallow, natural and man-made non-lotic waterbodies, which are threatened by blooms of Cyanoprokaryota/Cyanobacteria. Although cyanotoxins in Bulgarian surface waters are receiving increased attention, there is no information on microviridins and their producers. This paper presents results from a phytoplankton study, conducted in August 2019 in three lakes (Durankulak, Vaya, Uzungeren) and five reservoirs (Duvanli, Mandra, Poroy, Sinyata Reka, Zhrebchevo) in which a molecular-genetic analysis (PCR based on the precursor mdnA gene and subsequent translation to amino acid alignments), combined with conventional light microscopy and an HPLC analysis of marker pigments, were applied for the identification of potential microviridin producers. The results provide evidence that ten strains of the genus Microcystis, and of its most widespread species M. aeruginosa in particular, are potentially toxigenic in respect to microviridins. The mdnA sequences were obtained from all studied waterbodies and their translation to amino-acid alignments revealed the presence of five microviridin variants (types B/C, Izancya, CBJ55500.1 (Microcystis 199), and MC19, as well as a variant, which was very close to type A). This study adds to the general understanding of the microviridin occurrence, producers, and sequence diversity.
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spelling pubmed-83100142021-07-25 First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies Uzunov, Blagoy Stefanova, Katerina Radkova, Mariana Descy, Jean-Pierre Gärtner, Georg Stoyneva-Gärtner, Maya Toxins (Basel) Article Bulgaria, situated on the Balkan Peninsula, is rich in small and shallow, natural and man-made non-lotic waterbodies, which are threatened by blooms of Cyanoprokaryota/Cyanobacteria. Although cyanotoxins in Bulgarian surface waters are receiving increased attention, there is no information on microviridins and their producers. This paper presents results from a phytoplankton study, conducted in August 2019 in three lakes (Durankulak, Vaya, Uzungeren) and five reservoirs (Duvanli, Mandra, Poroy, Sinyata Reka, Zhrebchevo) in which a molecular-genetic analysis (PCR based on the precursor mdnA gene and subsequent translation to amino acid alignments), combined with conventional light microscopy and an HPLC analysis of marker pigments, were applied for the identification of potential microviridin producers. The results provide evidence that ten strains of the genus Microcystis, and of its most widespread species M. aeruginosa in particular, are potentially toxigenic in respect to microviridins. The mdnA sequences were obtained from all studied waterbodies and their translation to amino-acid alignments revealed the presence of five microviridin variants (types B/C, Izancya, CBJ55500.1 (Microcystis 199), and MC19, as well as a variant, which was very close to type A). This study adds to the general understanding of the microviridin occurrence, producers, and sequence diversity. MDPI 2021-06-28 /pmc/articles/PMC8310014/ /pubmed/34203459 http://dx.doi.org/10.3390/toxins13070448 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Uzunov, Blagoy
Stefanova, Katerina
Radkova, Mariana
Descy, Jean-Pierre
Gärtner, Georg
Stoyneva-Gärtner, Maya
First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies
title First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies
title_full First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies
title_fullStr First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies
title_full_unstemmed First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies
title_short First Report on Microcystis as a Potential Microviridin Producer in Bulgarian Waterbodies
title_sort first report on microcystis as a potential microviridin producer in bulgarian waterbodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310014/
https://www.ncbi.nlm.nih.gov/pubmed/34203459
http://dx.doi.org/10.3390/toxins13070448
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