Cargando…

Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1

Vitis vinifera represents an important and renowned source of compounds with significant biological activity. Wines and winery bioproducts, such as grape pomace, skins, and seeds, are rich in bioactive compounds against a wide range of human pathogens, including bacteria, fungi, and viruses. However...

Descripción completa

Detalles Bibliográficos
Autores principales: Zannella, Carla, Giugliano, Rosa, Chianese, Annalisa, Buonocore, Carmine, Vitale, Giovanni Andrea, Sanna, Giuseppina, Sarno, Federica, Manzin, Aldo, Nebbioso, Angela, Termolino, Pasquale, Altucci, Lucia, Galdiero, Massimiliano, de Pascale, Donatella, Franci, Gianluigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310055/
https://www.ncbi.nlm.nih.gov/pubmed/34209556
http://dx.doi.org/10.3390/v13071263
_version_ 1783728668429778944
author Zannella, Carla
Giugliano, Rosa
Chianese, Annalisa
Buonocore, Carmine
Vitale, Giovanni Andrea
Sanna, Giuseppina
Sarno, Federica
Manzin, Aldo
Nebbioso, Angela
Termolino, Pasquale
Altucci, Lucia
Galdiero, Massimiliano
de Pascale, Donatella
Franci, Gianluigi
author_facet Zannella, Carla
Giugliano, Rosa
Chianese, Annalisa
Buonocore, Carmine
Vitale, Giovanni Andrea
Sanna, Giuseppina
Sarno, Federica
Manzin, Aldo
Nebbioso, Angela
Termolino, Pasquale
Altucci, Lucia
Galdiero, Massimiliano
de Pascale, Donatella
Franci, Gianluigi
author_sort Zannella, Carla
collection PubMed
description Vitis vinifera represents an important and renowned source of compounds with significant biological activity. Wines and winery bioproducts, such as grape pomace, skins, and seeds, are rich in bioactive compounds against a wide range of human pathogens, including bacteria, fungi, and viruses. However, little is known about the biological properties of vine leaves. The aim of this study was the evaluation of phenolic composition and antiviral activity of Vitis vinifera leaf extract against two human viruses: the Herpes simplex virus type 1 (HSV-1) and the pandemic and currently widespread severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). About 40 phenolic compounds were identified in the extract by HPLC-MS/MS analysis: most of them were quercetin derivatives, others included derivatives of luteolin, kaempferol, apigenin, isorhamnetin, myricetin, chrysoeriol, biochanin, isookanin, and scutellarein. Leaf extract was able to inhibit both HSV-1 and SARS-CoV-2 replication in the early stages of infection by directly blocking the proteins enriched on the viral surface, at a very low concentration of 10 μg/mL. These results are very promising and highlight how natural extracts could be used in the design of antiviral drugs and the development of future vaccines.
format Online
Article
Text
id pubmed-8310055
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83100552021-07-25 Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1 Zannella, Carla Giugliano, Rosa Chianese, Annalisa Buonocore, Carmine Vitale, Giovanni Andrea Sanna, Giuseppina Sarno, Federica Manzin, Aldo Nebbioso, Angela Termolino, Pasquale Altucci, Lucia Galdiero, Massimiliano de Pascale, Donatella Franci, Gianluigi Viruses Article Vitis vinifera represents an important and renowned source of compounds with significant biological activity. Wines and winery bioproducts, such as grape pomace, skins, and seeds, are rich in bioactive compounds against a wide range of human pathogens, including bacteria, fungi, and viruses. However, little is known about the biological properties of vine leaves. The aim of this study was the evaluation of phenolic composition and antiviral activity of Vitis vinifera leaf extract against two human viruses: the Herpes simplex virus type 1 (HSV-1) and the pandemic and currently widespread severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). About 40 phenolic compounds were identified in the extract by HPLC-MS/MS analysis: most of them were quercetin derivatives, others included derivatives of luteolin, kaempferol, apigenin, isorhamnetin, myricetin, chrysoeriol, biochanin, isookanin, and scutellarein. Leaf extract was able to inhibit both HSV-1 and SARS-CoV-2 replication in the early stages of infection by directly blocking the proteins enriched on the viral surface, at a very low concentration of 10 μg/mL. These results are very promising and highlight how natural extracts could be used in the design of antiviral drugs and the development of future vaccines. MDPI 2021-06-29 /pmc/articles/PMC8310055/ /pubmed/34209556 http://dx.doi.org/10.3390/v13071263 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zannella, Carla
Giugliano, Rosa
Chianese, Annalisa
Buonocore, Carmine
Vitale, Giovanni Andrea
Sanna, Giuseppina
Sarno, Federica
Manzin, Aldo
Nebbioso, Angela
Termolino, Pasquale
Altucci, Lucia
Galdiero, Massimiliano
de Pascale, Donatella
Franci, Gianluigi
Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1
title Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1
title_full Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1
title_fullStr Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1
title_full_unstemmed Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1
title_short Antiviral Activity of Vitis vinifera Leaf Extract against SARS-CoV-2 and HSV-1
title_sort antiviral activity of vitis vinifera leaf extract against sars-cov-2 and hsv-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310055/
https://www.ncbi.nlm.nih.gov/pubmed/34209556
http://dx.doi.org/10.3390/v13071263
work_keys_str_mv AT zannellacarla antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT giuglianorosa antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT chianeseannalisa antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT buonocorecarmine antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT vitalegiovanniandrea antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT sannagiuseppina antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT sarnofederica antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT manzinaldo antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT nebbiosoangela antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT termolinopasquale antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT altuccilucia antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT galdieromassimiliano antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT depascaledonatella antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1
AT francigianluigi antiviralactivityofvitisviniferaleafextractagainstsarscov2andhsv1