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Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies

A universal influenza candidate vaccine that targets multiple conserved influenza virus epitopes from hemagglutinin (HA), neuraminidase (NA) and matrix (M2e) proteins was combined with the potent Army liposomal adjuvant (ALFQ) to promote induction of broad immunity to seasonal and pandemic influenza...

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Autores principales: Sei, Clara J., Rao, Mangala, Schuman, Richard F., Daum, Luke T., Matyas, Gary R., Rikhi, Nimisha, Muema, Kevin, Anderson, Alexander, Jobe, Ousman, Kroscher, Kellie A., Alving, Carl R., Fischer, Gerald W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310080/
https://www.ncbi.nlm.nih.gov/pubmed/34202178
http://dx.doi.org/10.3390/vaccines9070698
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author Sei, Clara J.
Rao, Mangala
Schuman, Richard F.
Daum, Luke T.
Matyas, Gary R.
Rikhi, Nimisha
Muema, Kevin
Anderson, Alexander
Jobe, Ousman
Kroscher, Kellie A.
Alving, Carl R.
Fischer, Gerald W.
author_facet Sei, Clara J.
Rao, Mangala
Schuman, Richard F.
Daum, Luke T.
Matyas, Gary R.
Rikhi, Nimisha
Muema, Kevin
Anderson, Alexander
Jobe, Ousman
Kroscher, Kellie A.
Alving, Carl R.
Fischer, Gerald W.
author_sort Sei, Clara J.
collection PubMed
description A universal influenza candidate vaccine that targets multiple conserved influenza virus epitopes from hemagglutinin (HA), neuraminidase (NA) and matrix (M2e) proteins was combined with the potent Army liposomal adjuvant (ALFQ) to promote induction of broad immunity to seasonal and pandemic influenza strains. The unconjugated and CRM-conjugated composite peptides formulated with ALFQ were highly immunogenic and induced both humoral and cellular immune responses in mice. Broadly reactive serum antibodies were induced across various IgG isotypes. Mice immunized with the unconjugated composite peptide developed antibody responses earlier than mice immunized with conjugated peptides, and the IgG antibodies were broadly reactive and neutralizing across Groups 1 and 2 influenza viruses. Multi-epitope unconjugated influenza composite peptides formulated with ALFQ provide a novel strategy for the development of a universal influenza vaccine. These synthetic peptide vaccines avoid the pitfalls of egg-produced influenza vaccines and production can be scaled up rapidly and economically.
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spelling pubmed-83100802021-07-25 Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies Sei, Clara J. Rao, Mangala Schuman, Richard F. Daum, Luke T. Matyas, Gary R. Rikhi, Nimisha Muema, Kevin Anderson, Alexander Jobe, Ousman Kroscher, Kellie A. Alving, Carl R. Fischer, Gerald W. Vaccines (Basel) Article A universal influenza candidate vaccine that targets multiple conserved influenza virus epitopes from hemagglutinin (HA), neuraminidase (NA) and matrix (M2e) proteins was combined with the potent Army liposomal adjuvant (ALFQ) to promote induction of broad immunity to seasonal and pandemic influenza strains. The unconjugated and CRM-conjugated composite peptides formulated with ALFQ were highly immunogenic and induced both humoral and cellular immune responses in mice. Broadly reactive serum antibodies were induced across various IgG isotypes. Mice immunized with the unconjugated composite peptide developed antibody responses earlier than mice immunized with conjugated peptides, and the IgG antibodies were broadly reactive and neutralizing across Groups 1 and 2 influenza viruses. Multi-epitope unconjugated influenza composite peptides formulated with ALFQ provide a novel strategy for the development of a universal influenza vaccine. These synthetic peptide vaccines avoid the pitfalls of egg-produced influenza vaccines and production can be scaled up rapidly and economically. MDPI 2021-06-25 /pmc/articles/PMC8310080/ /pubmed/34202178 http://dx.doi.org/10.3390/vaccines9070698 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sei, Clara J.
Rao, Mangala
Schuman, Richard F.
Daum, Luke T.
Matyas, Gary R.
Rikhi, Nimisha
Muema, Kevin
Anderson, Alexander
Jobe, Ousman
Kroscher, Kellie A.
Alving, Carl R.
Fischer, Gerald W.
Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies
title Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies
title_full Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies
title_fullStr Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies
title_full_unstemmed Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies
title_short Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies
title_sort conserved influenza hemagglutinin, neuraminidase and matrix peptides adjuvanted with alfq induce broadly neutralizing antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310080/
https://www.ncbi.nlm.nih.gov/pubmed/34202178
http://dx.doi.org/10.3390/vaccines9070698
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