Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources

The yellow fever virus vaccine, 17D, was derived through the serial passage of the wild-type (WT) strain Asibi virus in mouse and chicken tissue. Since its derivation, the mechanism of attenuation of 17D virus has been investigated using three 17D substrains and WT Asibi virus. Although all three su...

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Autores principales: Davis, Emily H., Thompson, Jill K., Widen, Steven G., Barrett, Alan D. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310116/
https://www.ncbi.nlm.nih.gov/pubmed/34372589
http://dx.doi.org/10.3390/v13071383
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author Davis, Emily H.
Thompson, Jill K.
Widen, Steven G.
Barrett, Alan D. T.
author_facet Davis, Emily H.
Thompson, Jill K.
Widen, Steven G.
Barrett, Alan D. T.
author_sort Davis, Emily H.
collection PubMed
description The yellow fever virus vaccine, 17D, was derived through the serial passage of the wild-type (WT) strain Asibi virus in mouse and chicken tissue. Since its derivation, the mechanism of attenuation of 17D virus has been investigated using three 17D substrains and WT Asibi virus. Although all three substrains of 17D have been sequenced, only one isolate of Asibi has been examined genetically and all interpretation of attenuation is based on this one isolate. Here, we sequenced the genome of Asibi virus from three different laboratories and show that the WT strain is genetically homogenous at the amino acids that distinguish Asibi from 17D vaccine virus.
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spelling pubmed-83101162021-07-25 Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources Davis, Emily H. Thompson, Jill K. Widen, Steven G. Barrett, Alan D. T. Viruses Brief Report The yellow fever virus vaccine, 17D, was derived through the serial passage of the wild-type (WT) strain Asibi virus in mouse and chicken tissue. Since its derivation, the mechanism of attenuation of 17D virus has been investigated using three 17D substrains and WT Asibi virus. Although all three substrains of 17D have been sequenced, only one isolate of Asibi has been examined genetically and all interpretation of attenuation is based on this one isolate. Here, we sequenced the genome of Asibi virus from three different laboratories and show that the WT strain is genetically homogenous at the amino acids that distinguish Asibi from 17D vaccine virus. MDPI 2021-07-16 /pmc/articles/PMC8310116/ /pubmed/34372589 http://dx.doi.org/10.3390/v13071383 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Davis, Emily H.
Thompson, Jill K.
Widen, Steven G.
Barrett, Alan D. T.
Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources
title Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources
title_full Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources
title_fullStr Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources
title_full_unstemmed Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources
title_short Genome Characterization of Yellow Fever Virus Wild-Type Strain Asibi, Parent to Live-Attenuated 17D Vaccine, from Three Different Sources
title_sort genome characterization of yellow fever virus wild-type strain asibi, parent to live-attenuated 17d vaccine, from three different sources
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310116/
https://www.ncbi.nlm.nih.gov/pubmed/34372589
http://dx.doi.org/10.3390/v13071383
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