Cargando…
Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection
The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310188/ https://www.ncbi.nlm.nih.gov/pubmed/34357939 http://dx.doi.org/10.3390/toxins13070467 |
_version_ | 1783728700627353600 |
---|---|
author | Ichihara, Aina Ojima, Hinako Gotoh, Kazuyoshi Matsushita, Osamu Take, Susumu Okada, Hiroyuki Watanabe, Akari Yokota, Kenji |
author_facet | Ichihara, Aina Ojima, Hinako Gotoh, Kazuyoshi Matsushita, Osamu Take, Susumu Okada, Hiroyuki Watanabe, Akari Yokota, Kenji |
author_sort | Ichihara, Aina |
collection | PubMed |
description | The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori. |
format | Online Article Text |
id | pubmed-8310188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83101882021-07-25 Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection Ichihara, Aina Ojima, Hinako Gotoh, Kazuyoshi Matsushita, Osamu Take, Susumu Okada, Hiroyuki Watanabe, Akari Yokota, Kenji Toxins (Basel) Article The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori. MDPI 2021-07-05 /pmc/articles/PMC8310188/ /pubmed/34357939 http://dx.doi.org/10.3390/toxins13070467 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ichihara, Aina Ojima, Hinako Gotoh, Kazuyoshi Matsushita, Osamu Take, Susumu Okada, Hiroyuki Watanabe, Akari Yokota, Kenji Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection |
title | Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection |
title_full | Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection |
title_fullStr | Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection |
title_full_unstemmed | Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection |
title_short | Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection |
title_sort | serodiagnosis and bacterial genome of helicobacter pylori infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310188/ https://www.ncbi.nlm.nih.gov/pubmed/34357939 http://dx.doi.org/10.3390/toxins13070467 |
work_keys_str_mv | AT ichiharaaina serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT ojimahinako serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT gotohkazuyoshi serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT matsushitaosamu serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT takesusumu serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT okadahiroyuki serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT watanabeakari serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection AT yokotakenji serodiagnosisandbacterialgenomeofhelicobacterpyloriinfection |