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Roles of the Endogenous Lunapark Protein during Flavivirus Replication
The endoplasmic reticulum (ER) of eukaryotic cells is a dynamic organelle, which undergoes continuous remodeling. At the three-way tubular junctions of the ER, the lunapark (LNP) protein acts as a membrane remodeling factor to stabilize these highly curved membrane junctions. In addition, during fla...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310331/ https://www.ncbi.nlm.nih.gov/pubmed/34206552 http://dx.doi.org/10.3390/v13071198 |
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author | Tran, Pham-Tue-Hung Asghar, Naveed Johansson, Magnus Melik, Wessam |
author_facet | Tran, Pham-Tue-Hung Asghar, Naveed Johansson, Magnus Melik, Wessam |
author_sort | Tran, Pham-Tue-Hung |
collection | PubMed |
description | The endoplasmic reticulum (ER) of eukaryotic cells is a dynamic organelle, which undergoes continuous remodeling. At the three-way tubular junctions of the ER, the lunapark (LNP) protein acts as a membrane remodeling factor to stabilize these highly curved membrane junctions. In addition, during flavivirus infection, the ER membrane is invaginated to form vesicles (Ve) for virus replication. Thus, LNP may have roles in the generation or maintenance of the Ve during flavivirus infection. In this study, our aim was to characterize the functions of LNP during flavivirus infection and investigate the underlying mechanisms of these functions. To specifically study virus replication, we generated cell lines expressing replicons of West Nile virus (Kunjin strain) or Langat virus. By using these replicon platforms and electron microscopy, we showed that depletion of LNP resulted in reduced virus replication, which is due to its role in the generation of the Ve. By using biochemical assays and high-resolution microscopy, we found that LNP is recruited to the Ve and the protein interacts with the nonstructural protein (NS) 4B. Therefore, these data shed new light on the interactions between flavivirus and host factors during viral replication. |
format | Online Article Text |
id | pubmed-8310331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83103312021-07-25 Roles of the Endogenous Lunapark Protein during Flavivirus Replication Tran, Pham-Tue-Hung Asghar, Naveed Johansson, Magnus Melik, Wessam Viruses Article The endoplasmic reticulum (ER) of eukaryotic cells is a dynamic organelle, which undergoes continuous remodeling. At the three-way tubular junctions of the ER, the lunapark (LNP) protein acts as a membrane remodeling factor to stabilize these highly curved membrane junctions. In addition, during flavivirus infection, the ER membrane is invaginated to form vesicles (Ve) for virus replication. Thus, LNP may have roles in the generation or maintenance of the Ve during flavivirus infection. In this study, our aim was to characterize the functions of LNP during flavivirus infection and investigate the underlying mechanisms of these functions. To specifically study virus replication, we generated cell lines expressing replicons of West Nile virus (Kunjin strain) or Langat virus. By using these replicon platforms and electron microscopy, we showed that depletion of LNP resulted in reduced virus replication, which is due to its role in the generation of the Ve. By using biochemical assays and high-resolution microscopy, we found that LNP is recruited to the Ve and the protein interacts with the nonstructural protein (NS) 4B. Therefore, these data shed new light on the interactions between flavivirus and host factors during viral replication. MDPI 2021-06-22 /pmc/articles/PMC8310331/ /pubmed/34206552 http://dx.doi.org/10.3390/v13071198 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tran, Pham-Tue-Hung Asghar, Naveed Johansson, Magnus Melik, Wessam Roles of the Endogenous Lunapark Protein during Flavivirus Replication |
title | Roles of the Endogenous Lunapark Protein during Flavivirus Replication |
title_full | Roles of the Endogenous Lunapark Protein during Flavivirus Replication |
title_fullStr | Roles of the Endogenous Lunapark Protein during Flavivirus Replication |
title_full_unstemmed | Roles of the Endogenous Lunapark Protein during Flavivirus Replication |
title_short | Roles of the Endogenous Lunapark Protein during Flavivirus Replication |
title_sort | roles of the endogenous lunapark protein during flavivirus replication |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310331/ https://www.ncbi.nlm.nih.gov/pubmed/34206552 http://dx.doi.org/10.3390/v13071198 |
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