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Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy
Influenza viruses cause respiratory infections in humans and animals, which have high morbidity and mortality rates. Although several drugs that inhibit viral neuraminidase are used to treat influenza infections, the emergence of resistant viruses necessitates the urgent development of new antiviral...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310364/ https://www.ncbi.nlm.nih.gov/pubmed/34372556 http://dx.doi.org/10.3390/v13071350 |
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author | Kim, Seong-Ryeol Jeong, Myeong-Seon Mun, Seo-Hyeon Cho, Jaewon Seo, Min-Duk Kim, Hyoungsu Lee, Jooeun Song, Jae-Hyoung Ko, Hyun-Jeong |
author_facet | Kim, Seong-Ryeol Jeong, Myeong-Seon Mun, Seo-Hyeon Cho, Jaewon Seo, Min-Duk Kim, Hyoungsu Lee, Jooeun Song, Jae-Hyoung Ko, Hyun-Jeong |
author_sort | Kim, Seong-Ryeol |
collection | PubMed |
description | Influenza viruses cause respiratory infections in humans and animals, which have high morbidity and mortality rates. Although several drugs that inhibit viral neuraminidase are used to treat influenza infections, the emergence of resistant viruses necessitates the urgent development of new antiviral drugs. Chrysin (5,7-dihydroxyflavone) is a natural flavonoid that exhibits antiviral activity against enterovirus 71 (EV71) by inhibiting viral 3C protease activity. In this study, we evaluated the antiviral activity of chrysin against influenza A/Puerto Rico/8/34 (A/PR/8). Chrysin significantly inhibited A/PR/8-mediated cell death and the replication of A/PR/8 at concentrations up to 2 μM. Viral hemagglutinin expression was also markedly decreased by the chrysin treatment in A/PR/8-infected cells. Through the time course experiment and time-of-addition assay, we found that chrysin inhibited viral infection at the early stages of the replication cycle. Additionally, the nucleoprotein expression of A/PR/8 in A549 cells was reduced upon treatment with chrysin. Regarding the mechanism of action, we found that chrysin inhibited autophagy activation by increasing the phosphorylation of mammalian target of rapamycin (mTOR). We also confirmed a decrease in LC3B expression and LC3-positive puncta levels in A/PR/8-infected cells. These results suggest that chrysin exhibits antiviral activity by activating mTOR and inhibiting autophagy to inhibit the replication of A/PR/8 in the early stages of infection. |
format | Online Article Text |
id | pubmed-8310364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83103642021-07-25 Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy Kim, Seong-Ryeol Jeong, Myeong-Seon Mun, Seo-Hyeon Cho, Jaewon Seo, Min-Duk Kim, Hyoungsu Lee, Jooeun Song, Jae-Hyoung Ko, Hyun-Jeong Viruses Article Influenza viruses cause respiratory infections in humans and animals, which have high morbidity and mortality rates. Although several drugs that inhibit viral neuraminidase are used to treat influenza infections, the emergence of resistant viruses necessitates the urgent development of new antiviral drugs. Chrysin (5,7-dihydroxyflavone) is a natural flavonoid that exhibits antiviral activity against enterovirus 71 (EV71) by inhibiting viral 3C protease activity. In this study, we evaluated the antiviral activity of chrysin against influenza A/Puerto Rico/8/34 (A/PR/8). Chrysin significantly inhibited A/PR/8-mediated cell death and the replication of A/PR/8 at concentrations up to 2 μM. Viral hemagglutinin expression was also markedly decreased by the chrysin treatment in A/PR/8-infected cells. Through the time course experiment and time-of-addition assay, we found that chrysin inhibited viral infection at the early stages of the replication cycle. Additionally, the nucleoprotein expression of A/PR/8 in A549 cells was reduced upon treatment with chrysin. Regarding the mechanism of action, we found that chrysin inhibited autophagy activation by increasing the phosphorylation of mammalian target of rapamycin (mTOR). We also confirmed a decrease in LC3B expression and LC3-positive puncta levels in A/PR/8-infected cells. These results suggest that chrysin exhibits antiviral activity by activating mTOR and inhibiting autophagy to inhibit the replication of A/PR/8 in the early stages of infection. MDPI 2021-07-13 /pmc/articles/PMC8310364/ /pubmed/34372556 http://dx.doi.org/10.3390/v13071350 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Seong-Ryeol Jeong, Myeong-Seon Mun, Seo-Hyeon Cho, Jaewon Seo, Min-Duk Kim, Hyoungsu Lee, Jooeun Song, Jae-Hyoung Ko, Hyun-Jeong Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy |
title | Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy |
title_full | Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy |
title_fullStr | Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy |
title_full_unstemmed | Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy |
title_short | Antiviral Activity of Chrysin against Influenza Virus Replication via Inhibition of Autophagy |
title_sort | antiviral activity of chrysin against influenza virus replication via inhibition of autophagy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310364/ https://www.ncbi.nlm.nih.gov/pubmed/34372556 http://dx.doi.org/10.3390/v13071350 |
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