Cargando…
Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia
Arthropod-borne infections are a medical and economic threat to humans and livestock. Over the last three decades, several unprecedented viral outbreaks have been recorded in the Western part of the Arabian Peninsula. However, little is known about the circulation and diversity of arthropod-borne vi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310382/ https://www.ncbi.nlm.nih.gov/pubmed/34372602 http://dx.doi.org/10.3390/v13071396 |
_version_ | 1783728748006211584 |
---|---|
author | Zakham, Fathiah Albalawi, Aishah E. Alanazi, Abdullah D. Truong Nguyen, Phuoc Alouffi, Abdulaziz S. Alaoui, Altaf Sironen, Tarja Smura, Teemu Vapalahti, Olli |
author_facet | Zakham, Fathiah Albalawi, Aishah E. Alanazi, Abdullah D. Truong Nguyen, Phuoc Alouffi, Abdulaziz S. Alaoui, Altaf Sironen, Tarja Smura, Teemu Vapalahti, Olli |
author_sort | Zakham, Fathiah |
collection | PubMed |
description | Arthropod-borne infections are a medical and economic threat to humans and livestock. Over the last three decades, several unprecedented viral outbreaks have been recorded in the Western part of the Arabian Peninsula. However, little is known about the circulation and diversity of arthropod-borne viruses in this region. To prepare for new outbreaks of vector-borne diseases, it is important to detect which viruses circulate in each vector population. In this study, we used a metagenomics approach to characterize the RNA virome of ticks infesting dromedary camels (Camelus dromedaries) in Makkah province, Saudi Arabia. Two hundred ticks of species Hyalomma dromedarii (n = 196) and Hyalomma impeltatum (n = 4) were collected from the Alkhurma district in Jeddah and Al-Taif city. Virome analysis showed the presence of several tick-specific viruses and tick-borne viruses associated with severe illness in humans. Some were identified for the first time in the Arabian Peninsula. The human disease-associated viruses detected included Crimean Congo Hemorrhagic fever virus and Tamdy virus (family Nairoviridae), Guertu virus (family Phenuiviridae), and a novel coltivirus that shares similarities with Tarumizu virus, Tai forest reovirus and Kundal virus (family Reoviridae). Furthermore, Alkhurma hemorrhagic virus (Flaviviridae) was detected in two tick pools by specific qPCR. In addition, tick-specific viruses in families Phenuiviridae (phleboviruses), Iflaviridae, Chuviridae, Totiviridae and Flaviviridae (Pestivirus) were detected. The presence of human pathogenetic viruses warrants further efforts in tick surveillance, xenosurveillence, vector control, and sero-epidemiological investigations in human and animal populations to predict, contain and mitigate future outbreaks in the region. |
format | Online Article Text |
id | pubmed-8310382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83103822021-07-25 Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia Zakham, Fathiah Albalawi, Aishah E. Alanazi, Abdullah D. Truong Nguyen, Phuoc Alouffi, Abdulaziz S. Alaoui, Altaf Sironen, Tarja Smura, Teemu Vapalahti, Olli Viruses Article Arthropod-borne infections are a medical and economic threat to humans and livestock. Over the last three decades, several unprecedented viral outbreaks have been recorded in the Western part of the Arabian Peninsula. However, little is known about the circulation and diversity of arthropod-borne viruses in this region. To prepare for new outbreaks of vector-borne diseases, it is important to detect which viruses circulate in each vector population. In this study, we used a metagenomics approach to characterize the RNA virome of ticks infesting dromedary camels (Camelus dromedaries) in Makkah province, Saudi Arabia. Two hundred ticks of species Hyalomma dromedarii (n = 196) and Hyalomma impeltatum (n = 4) were collected from the Alkhurma district in Jeddah and Al-Taif city. Virome analysis showed the presence of several tick-specific viruses and tick-borne viruses associated with severe illness in humans. Some were identified for the first time in the Arabian Peninsula. The human disease-associated viruses detected included Crimean Congo Hemorrhagic fever virus and Tamdy virus (family Nairoviridae), Guertu virus (family Phenuiviridae), and a novel coltivirus that shares similarities with Tarumizu virus, Tai forest reovirus and Kundal virus (family Reoviridae). Furthermore, Alkhurma hemorrhagic virus (Flaviviridae) was detected in two tick pools by specific qPCR. In addition, tick-specific viruses in families Phenuiviridae (phleboviruses), Iflaviridae, Chuviridae, Totiviridae and Flaviviridae (Pestivirus) were detected. The presence of human pathogenetic viruses warrants further efforts in tick surveillance, xenosurveillence, vector control, and sero-epidemiological investigations in human and animal populations to predict, contain and mitigate future outbreaks in the region. MDPI 2021-07-18 /pmc/articles/PMC8310382/ /pubmed/34372602 http://dx.doi.org/10.3390/v13071396 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zakham, Fathiah Albalawi, Aishah E. Alanazi, Abdullah D. Truong Nguyen, Phuoc Alouffi, Abdulaziz S. Alaoui, Altaf Sironen, Tarja Smura, Teemu Vapalahti, Olli Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia |
title | Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia |
title_full | Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia |
title_fullStr | Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia |
title_full_unstemmed | Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia |
title_short | Viral RNA Metagenomics of Hyalomma Ticks Collected from Dromedary Camels in Makkah Province, Saudi Arabia |
title_sort | viral rna metagenomics of hyalomma ticks collected from dromedary camels in makkah province, saudi arabia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310382/ https://www.ncbi.nlm.nih.gov/pubmed/34372602 http://dx.doi.org/10.3390/v13071396 |
work_keys_str_mv | AT zakhamfathiah viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT albalawiaishahe viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT alanaziabdullahd viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT truongnguyenphuoc viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT alouffiabdulazizs viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT alaouialtaf viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT sironentarja viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT smurateemu viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia AT vapalahtiolli viralrnametagenomicsofhyalommatickscollectedfromdromedarycamelsinmakkahprovincesaudiarabia |