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Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil

BACKGROUND: The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected in...

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Autores principales: Mota, Magaly Lima, dos Santos Souza Marinho, Robson, Duro, Rodrigo Lopes Sanz, Hunter, James, de Menezes, Irwin Rose Alencar, de Lima Silva, João Marcos Ferreira, Pereira, Glaubervânio Leite Tavares, Sabino, Ester Cerdeira, Grumach, Anete, Diaz, Ricardo Sobhie, do Socorro Lucena, Maria, Komninakis, Shirley Vasconcelos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310596/
https://www.ncbi.nlm.nih.gov/pubmed/34303348
http://dx.doi.org/10.1186/s12879-021-06401-3
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author Mota, Magaly Lima
dos Santos Souza Marinho, Robson
Duro, Rodrigo Lopes Sanz
Hunter, James
de Menezes, Irwin Rose Alencar
de Lima Silva, João Marcos Ferreira
Pereira, Glaubervânio Leite Tavares
Sabino, Ester Cerdeira
Grumach, Anete
Diaz, Ricardo Sobhie
do Socorro Lucena, Maria
Komninakis, Shirley Vasconcelos
author_facet Mota, Magaly Lima
dos Santos Souza Marinho, Robson
Duro, Rodrigo Lopes Sanz
Hunter, James
de Menezes, Irwin Rose Alencar
de Lima Silva, João Marcos Ferreira
Pereira, Glaubervânio Leite Tavares
Sabino, Ester Cerdeira
Grumach, Anete
Diaz, Ricardo Sobhie
do Socorro Lucena, Maria
Komninakis, Shirley Vasconcelos
author_sort Mota, Magaly Lima
collection PubMed
description BACKGROUND: The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil. METHODS: In 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval. RESULTS: In serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI: 45.76–70.56) than against DENV (95.05% with 95% CI: 78.09–98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence: mono-infection by ZIKV of 19.23% (95% CI: 14.29–34.82) patients, mono-infection by CHIKV of 3.84% (95% CI: 2.01–5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI: 0.89–4.56). CONCLUSION: The serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil.
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spelling pubmed-83105962021-07-28 Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil Mota, Magaly Lima dos Santos Souza Marinho, Robson Duro, Rodrigo Lopes Sanz Hunter, James de Menezes, Irwin Rose Alencar de Lima Silva, João Marcos Ferreira Pereira, Glaubervânio Leite Tavares Sabino, Ester Cerdeira Grumach, Anete Diaz, Ricardo Sobhie do Socorro Lucena, Maria Komninakis, Shirley Vasconcelos BMC Infect Dis Research BACKGROUND: The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil. METHODS: In 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval. RESULTS: In serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI: 45.76–70.56) than against DENV (95.05% with 95% CI: 78.09–98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence: mono-infection by ZIKV of 19.23% (95% CI: 14.29–34.82) patients, mono-infection by CHIKV of 3.84% (95% CI: 2.01–5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI: 0.89–4.56). CONCLUSION: The serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil. BioMed Central 2021-07-24 /pmc/articles/PMC8310596/ /pubmed/34303348 http://dx.doi.org/10.1186/s12879-021-06401-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mota, Magaly Lima
dos Santos Souza Marinho, Robson
Duro, Rodrigo Lopes Sanz
Hunter, James
de Menezes, Irwin Rose Alencar
de Lima Silva, João Marcos Ferreira
Pereira, Glaubervânio Leite Tavares
Sabino, Ester Cerdeira
Grumach, Anete
Diaz, Ricardo Sobhie
do Socorro Lucena, Maria
Komninakis, Shirley Vasconcelos
Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil
title Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil
title_full Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil
title_fullStr Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil
title_full_unstemmed Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil
title_short Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil
title_sort serological and molecular epidemiology of the dengue, zika and chikungunya viruses in a risk area in brazil
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310596/
https://www.ncbi.nlm.nih.gov/pubmed/34303348
http://dx.doi.org/10.1186/s12879-021-06401-3
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