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Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM

A novel machine learning algorithm is shown to accurately discriminate between oral squamous cell carcinoma (OSCC) nodal metastases and surrounding lymphoid tissue on the basis of a single metric, the ratio of Fourier transform infrared (FTIR) absorption intensities at 1252 cm(−1) and 1285 cm(−1). T...

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Autores principales: Ellis, Barnaby G., Whitley, Conor A., Al Jedani, Safaa, Smith, Caroline I., Gunning, Philip J., Harrison, Paul, Unsworth, Paul, Gardner, Peter, Shaw, Richard J., Barrett, Steve D., Triantafyllou, Asterios, Risk, Janet M., Weightman, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311263/
https://www.ncbi.nlm.nih.gov/pubmed/34241603
http://dx.doi.org/10.1039/d1an00922b
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author Ellis, Barnaby G.
Whitley, Conor A.
Al Jedani, Safaa
Smith, Caroline I.
Gunning, Philip J.
Harrison, Paul
Unsworth, Paul
Gardner, Peter
Shaw, Richard J.
Barrett, Steve D.
Triantafyllou, Asterios
Risk, Janet M.
Weightman, Peter
author_facet Ellis, Barnaby G.
Whitley, Conor A.
Al Jedani, Safaa
Smith, Caroline I.
Gunning, Philip J.
Harrison, Paul
Unsworth, Paul
Gardner, Peter
Shaw, Richard J.
Barrett, Steve D.
Triantafyllou, Asterios
Risk, Janet M.
Weightman, Peter
author_sort Ellis, Barnaby G.
collection PubMed
description A novel machine learning algorithm is shown to accurately discriminate between oral squamous cell carcinoma (OSCC) nodal metastases and surrounding lymphoid tissue on the basis of a single metric, the ratio of Fourier transform infrared (FTIR) absorption intensities at 1252 cm(−1) and 1285 cm(−1). The metric yields discriminating sensitivities, specificities and precision of 98.8 ± 0.1%, 99.89 ± 0.01% and 99.78 ± 0.02% respectively, and an area under receiver operator characteristic (AUC) of 0.9935 ± 0.0006. The delineation of the OSCC and lymphoid tissue revealed by the image formed from the metric is in better agreement with an immunohistochemistry (IHC) stained image than are either of the FTIR images obtained at the individual wavenumbers. Scanning near-field optical microscopy (SNOM) images of the tissue obtained at a number of key wavenumbers, with high spatial resolution, show variations in the chemical structure of the tissue with a feature size down to ∼4 μm. The image formed from the ratio of the SNOM images obtained at 1252 cm(−1) and 1285 cm(−1) shows more contrast than the SNOM images obtained at these or a number of other individual wavenumbers. The discrimination between the two tissue types is dominated by the contribution from the 1252 cm(−1) signal, which is representative of nucleic acids, and this shows the OSCC tissue to be accompanied by two wide arcs of tissue which are particularly low in nucleic acids. Haematoxylin and eosin (H&E) staining shows the tumour core in this specimen to be ∼40 μm wide and the SNOM topography shows that the core centre is raised by ∼1 μm compared to the surrounding tissue. Line profiles of the SNOM signal intensity taken through the highly keratinised core show that the increase in height correlates with an increase in the protein signal. SNOM line profiles show that the nucleic acids signal decreases at the centre of the tumour core between two peaks of higher intensity. All these nucleic acid features are ∼25 μm wide, roughly the width of two cancer cells.
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spelling pubmed-83112632021-08-03 Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM Ellis, Barnaby G. Whitley, Conor A. Al Jedani, Safaa Smith, Caroline I. Gunning, Philip J. Harrison, Paul Unsworth, Paul Gardner, Peter Shaw, Richard J. Barrett, Steve D. Triantafyllou, Asterios Risk, Janet M. Weightman, Peter Analyst Chemistry A novel machine learning algorithm is shown to accurately discriminate between oral squamous cell carcinoma (OSCC) nodal metastases and surrounding lymphoid tissue on the basis of a single metric, the ratio of Fourier transform infrared (FTIR) absorption intensities at 1252 cm(−1) and 1285 cm(−1). The metric yields discriminating sensitivities, specificities and precision of 98.8 ± 0.1%, 99.89 ± 0.01% and 99.78 ± 0.02% respectively, and an area under receiver operator characteristic (AUC) of 0.9935 ± 0.0006. The delineation of the OSCC and lymphoid tissue revealed by the image formed from the metric is in better agreement with an immunohistochemistry (IHC) stained image than are either of the FTIR images obtained at the individual wavenumbers. Scanning near-field optical microscopy (SNOM) images of the tissue obtained at a number of key wavenumbers, with high spatial resolution, show variations in the chemical structure of the tissue with a feature size down to ∼4 μm. The image formed from the ratio of the SNOM images obtained at 1252 cm(−1) and 1285 cm(−1) shows more contrast than the SNOM images obtained at these or a number of other individual wavenumbers. The discrimination between the two tissue types is dominated by the contribution from the 1252 cm(−1) signal, which is representative of nucleic acids, and this shows the OSCC tissue to be accompanied by two wide arcs of tissue which are particularly low in nucleic acids. Haematoxylin and eosin (H&E) staining shows the tumour core in this specimen to be ∼40 μm wide and the SNOM topography shows that the core centre is raised by ∼1 μm compared to the surrounding tissue. Line profiles of the SNOM signal intensity taken through the highly keratinised core show that the increase in height correlates with an increase in the protein signal. SNOM line profiles show that the nucleic acids signal decreases at the centre of the tumour core between two peaks of higher intensity. All these nucleic acid features are ∼25 μm wide, roughly the width of two cancer cells. The Royal Society of Chemistry 2021-07-05 /pmc/articles/PMC8311263/ /pubmed/34241603 http://dx.doi.org/10.1039/d1an00922b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Ellis, Barnaby G.
Whitley, Conor A.
Al Jedani, Safaa
Smith, Caroline I.
Gunning, Philip J.
Harrison, Paul
Unsworth, Paul
Gardner, Peter
Shaw, Richard J.
Barrett, Steve D.
Triantafyllou, Asterios
Risk, Janet M.
Weightman, Peter
Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM
title Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM
title_full Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM
title_fullStr Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM
title_full_unstemmed Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM
title_short Insight into metastatic oral cancer tissue from novel analyses using FTIR spectroscopy and aperture IR-SNOM
title_sort insight into metastatic oral cancer tissue from novel analyses using ftir spectroscopy and aperture ir-snom
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311263/
https://www.ncbi.nlm.nih.gov/pubmed/34241603
http://dx.doi.org/10.1039/d1an00922b
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