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Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control

Central micturition control and urine storage involve a multisynaptic neuronal circuit for the efferent control of the urinary bladder. Electrical stimulation of the lateral preoptic area (LPA) at the level of the decussation of the anterior commissure in cats evokes relaxation of the bladder, where...

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Autores principales: Lamy, Gustavo B., Cafarchio, Eduardo M., do Vale, Bárbara, Antonio, Bruno B., Venancio, Daniel P., de Souza, Janaina S., Maciel, Rui M., Giannocco, Gisele, Aronsson, Patrik, Sato, Monica A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311566/
https://www.ncbi.nlm.nih.gov/pubmed/34322035
http://dx.doi.org/10.3389/fphys.2021.682711
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author Lamy, Gustavo B.
Cafarchio, Eduardo M.
do Vale, Bárbara
Antonio, Bruno B.
Venancio, Daniel P.
de Souza, Janaina S.
Maciel, Rui M.
Giannocco, Gisele
Aronsson, Patrik
Sato, Monica A.
author_facet Lamy, Gustavo B.
Cafarchio, Eduardo M.
do Vale, Bárbara
Antonio, Bruno B.
Venancio, Daniel P.
de Souza, Janaina S.
Maciel, Rui M.
Giannocco, Gisele
Aronsson, Patrik
Sato, Monica A.
author_sort Lamy, Gustavo B.
collection PubMed
description Central micturition control and urine storage involve a multisynaptic neuronal circuit for the efferent control of the urinary bladder. Electrical stimulation of the lateral preoptic area (LPA) at the level of the decussation of the anterior commissure in cats evokes relaxation of the bladder, whereas ventral stimulation of LPA evokes vigorous contraction. Endogenous Angiotensin-(1–7) [(Ang-(1–7)] synthesis depends on ACE-2, and its actions on binding to Mas receptors, which were found in LPA neurons. We aimed to investigate the Ang-(1–7) actions into the LPA on intravesical pressure (IP) and cardiovascular parameters. The gene and protein expressions of Mas receptors and ACE-2 were also evaluated in the LPA. Angiotensin-(1–7) (5 nmol/μL) or A-779 (Mas receptor antagonist, 50 nmol/μL) was injected into the LPA in anesthetized female Wistar rats; and the IP, mean arterial pressure (MAP), heart rate (HR), and renal conductance (RC) were recorded for 30 min. Unilateral injection of Ang-(1–7) into the LPA increased IP (187.46 ± 37.23%) with peak response at ∼23–25-min post-injection and yielded no changes in MAP, HR, and RC. Unilateral or bilateral injections of A-779 into the LPA decreased IP (−15.88 ± 2.76 and −27.30 ± 3.40%, respectively) and elicited no changes in MAP, HR, and RC. The genes and the protein expression of Mas receptors and ACE-2 were found in the LPA. Therefore, the LPA is an important part of the circuit involved in the urinary bladder control, in which the Ang-(1–7) synthetized into the LPA activates Mas receptors for increasing the IP independent on changes in RC and cardiovascular parameters.
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spelling pubmed-83115662021-07-27 Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control Lamy, Gustavo B. Cafarchio, Eduardo M. do Vale, Bárbara Antonio, Bruno B. Venancio, Daniel P. de Souza, Janaina S. Maciel, Rui M. Giannocco, Gisele Aronsson, Patrik Sato, Monica A. Front Physiol Physiology Central micturition control and urine storage involve a multisynaptic neuronal circuit for the efferent control of the urinary bladder. Electrical stimulation of the lateral preoptic area (LPA) at the level of the decussation of the anterior commissure in cats evokes relaxation of the bladder, whereas ventral stimulation of LPA evokes vigorous contraction. Endogenous Angiotensin-(1–7) [(Ang-(1–7)] synthesis depends on ACE-2, and its actions on binding to Mas receptors, which were found in LPA neurons. We aimed to investigate the Ang-(1–7) actions into the LPA on intravesical pressure (IP) and cardiovascular parameters. The gene and protein expressions of Mas receptors and ACE-2 were also evaluated in the LPA. Angiotensin-(1–7) (5 nmol/μL) or A-779 (Mas receptor antagonist, 50 nmol/μL) was injected into the LPA in anesthetized female Wistar rats; and the IP, mean arterial pressure (MAP), heart rate (HR), and renal conductance (RC) were recorded for 30 min. Unilateral injection of Ang-(1–7) into the LPA increased IP (187.46 ± 37.23%) with peak response at ∼23–25-min post-injection and yielded no changes in MAP, HR, and RC. Unilateral or bilateral injections of A-779 into the LPA decreased IP (−15.88 ± 2.76 and −27.30 ± 3.40%, respectively) and elicited no changes in MAP, HR, and RC. The genes and the protein expression of Mas receptors and ACE-2 were found in the LPA. Therefore, the LPA is an important part of the circuit involved in the urinary bladder control, in which the Ang-(1–7) synthetized into the LPA activates Mas receptors for increasing the IP independent on changes in RC and cardiovascular parameters. Frontiers Media S.A. 2021-07-12 /pmc/articles/PMC8311566/ /pubmed/34322035 http://dx.doi.org/10.3389/fphys.2021.682711 Text en Copyright © 2021 Lamy, Cafarchio, do Vale, Antonio, Venancio, de Souza, Maciel, Giannocco, Aronsson and Sato. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Lamy, Gustavo B.
Cafarchio, Eduardo M.
do Vale, Bárbara
Antonio, Bruno B.
Venancio, Daniel P.
de Souza, Janaina S.
Maciel, Rui M.
Giannocco, Gisele
Aronsson, Patrik
Sato, Monica A.
Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control
title Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control
title_full Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control
title_fullStr Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control
title_full_unstemmed Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control
title_short Lateral Preoptic Area Neurons Activated by Angiotensin-(1–7) Increase Intravesical Pressure: A Novel Feature in Central Micturition Control
title_sort lateral preoptic area neurons activated by angiotensin-(1–7) increase intravesical pressure: a novel feature in central micturition control
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311566/
https://www.ncbi.nlm.nih.gov/pubmed/34322035
http://dx.doi.org/10.3389/fphys.2021.682711
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