Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis

Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibod...

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Autores principales: Bachtiar, Endang Winiati, Putri, Citra F., Soejoedono, Retno D., Bachtiar, Boy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311800/
https://www.ncbi.nlm.nih.gov/pubmed/34367622
http://dx.doi.org/10.12688/f1000research.26749.4
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author Bachtiar, Endang Winiati
Putri, Citra F.
Soejoedono, Retno D.
Bachtiar, Boy M.
author_facet Bachtiar, Endang Winiati
Putri, Citra F.
Soejoedono, Retno D.
Bachtiar, Boy M.
author_sort Bachtiar, Endang Winiati
collection PubMed
description Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B,  iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and  iNOS2 of neuron cells.
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spelling pubmed-83118002021-08-05 Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis Bachtiar, Endang Winiati Putri, Citra F. Soejoedono, Retno D. Bachtiar, Boy M. F1000Res Brief Report Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B,  iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and  iNOS2 of neuron cells. F1000 Research Limited 2021-06-28 /pmc/articles/PMC8311800/ /pubmed/34367622 http://dx.doi.org/10.12688/f1000research.26749.4 Text en Copyright: © 2021 Bachtiar EW et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Bachtiar, Endang Winiati
Putri, Citra F.
Soejoedono, Retno D.
Bachtiar, Boy M.
Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis
title Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis
title_full Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis
title_fullStr Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis
title_full_unstemmed Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis
title_short Expression of TNF, IL1B, and  iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis
title_sort expression of tnf, il1b, and  inos2 in the neural cell after induced by porphyromonas gingivalis with and without coating antibody anti -porphyromonas gingivalis
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311800/
https://www.ncbi.nlm.nih.gov/pubmed/34367622
http://dx.doi.org/10.12688/f1000research.26749.4
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