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Distinct microbial communities colonize tonsillar squamous cell carcinoma

Squamous cell carcinoma of the tonsil is one of the most frequent cancers of the oropharynx. The escalating rate of tonsil cancer during the last decades is associated with the increase of high risk-human papilloma virus (HR-HPV) infections. While the microbiome in oropharyngeal malignant diseases h...

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Autores principales: De Martin, Angelina, Lütge, Mechthild, Stanossek, Yves, Engetschwiler, Céline, Cupovic, Jovana, Brown, Kirsty, Demmer, Izadora, Broglie, Martina A., Geuking, Markus B., Jochum, Wolfram, McCoy, Kathy D., Stoeckli, Sandro J., Ludewig, Burkhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312615/
https://www.ncbi.nlm.nih.gov/pubmed/34367729
http://dx.doi.org/10.1080/2162402X.2021.1945202
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author De Martin, Angelina
Lütge, Mechthild
Stanossek, Yves
Engetschwiler, Céline
Cupovic, Jovana
Brown, Kirsty
Demmer, Izadora
Broglie, Martina A.
Geuking, Markus B.
Jochum, Wolfram
McCoy, Kathy D.
Stoeckli, Sandro J.
Ludewig, Burkhard
author_facet De Martin, Angelina
Lütge, Mechthild
Stanossek, Yves
Engetschwiler, Céline
Cupovic, Jovana
Brown, Kirsty
Demmer, Izadora
Broglie, Martina A.
Geuking, Markus B.
Jochum, Wolfram
McCoy, Kathy D.
Stoeckli, Sandro J.
Ludewig, Burkhard
author_sort De Martin, Angelina
collection PubMed
description Squamous cell carcinoma of the tonsil is one of the most frequent cancers of the oropharynx. The escalating rate of tonsil cancer during the last decades is associated with the increase of high risk-human papilloma virus (HR-HPV) infections. While the microbiome in oropharyngeal malignant diseases has been characterized to some extent, the microbial colonization of HR-HPV-associated tonsil cancer remains largely unknown. Using 16S rRNA gene amplicon sequencing, we have characterized the microbiome of human palatine tonsil crypts in patients suffering from HR-HPV-associated tonsil cancer in comparison to a control cohort of adult sleep apnea patients. We found an increased abundance of the phyla Firmicutes and Actinobacteria in tumor patients, whereas the abundance of Spirochetes and Synergistetes was significantly higher in the control cohort. Furthermore, the accumulation of several genera such as Veillonella, Streptococcus and Prevotella_7 in tonsillar crypts was associated with tonsil cancer. In contrast, Fusobacterium, Prevotella and Treponema_2 were enriched in sleep apnea patients. Machine learning-based bacterial species analysis indicated that a particular bacterial composition in tonsillar crypts is tumor-predictive. Species-specific PCR-based validation in extended patient cohorts confirmed that differential abundance of Filifactor alocis and Prevotella melaninogenica is a distinct trait of tonsil cancer. This study shows that tonsil cancer patients harbor a characteristic microbiome in the crypt environment that differs from the microbiome of sleep apnea patients on all phylogenetic levels. Moreover, our analysis indicates that profiling of microbial communities in distinct tonsillar niches provides microbiome-based avenues for the diagnosis of tonsil cancer.
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spelling pubmed-83126152021-08-06 Distinct microbial communities colonize tonsillar squamous cell carcinoma De Martin, Angelina Lütge, Mechthild Stanossek, Yves Engetschwiler, Céline Cupovic, Jovana Brown, Kirsty Demmer, Izadora Broglie, Martina A. Geuking, Markus B. Jochum, Wolfram McCoy, Kathy D. Stoeckli, Sandro J. Ludewig, Burkhard Oncoimmunology Original Research Squamous cell carcinoma of the tonsil is one of the most frequent cancers of the oropharynx. The escalating rate of tonsil cancer during the last decades is associated with the increase of high risk-human papilloma virus (HR-HPV) infections. While the microbiome in oropharyngeal malignant diseases has been characterized to some extent, the microbial colonization of HR-HPV-associated tonsil cancer remains largely unknown. Using 16S rRNA gene amplicon sequencing, we have characterized the microbiome of human palatine tonsil crypts in patients suffering from HR-HPV-associated tonsil cancer in comparison to a control cohort of adult sleep apnea patients. We found an increased abundance of the phyla Firmicutes and Actinobacteria in tumor patients, whereas the abundance of Spirochetes and Synergistetes was significantly higher in the control cohort. Furthermore, the accumulation of several genera such as Veillonella, Streptococcus and Prevotella_7 in tonsillar crypts was associated with tonsil cancer. In contrast, Fusobacterium, Prevotella and Treponema_2 were enriched in sleep apnea patients. Machine learning-based bacterial species analysis indicated that a particular bacterial composition in tonsillar crypts is tumor-predictive. Species-specific PCR-based validation in extended patient cohorts confirmed that differential abundance of Filifactor alocis and Prevotella melaninogenica is a distinct trait of tonsil cancer. This study shows that tonsil cancer patients harbor a characteristic microbiome in the crypt environment that differs from the microbiome of sleep apnea patients on all phylogenetic levels. Moreover, our analysis indicates that profiling of microbial communities in distinct tonsillar niches provides microbiome-based avenues for the diagnosis of tonsil cancer. Taylor & Francis 2021-07-25 /pmc/articles/PMC8312615/ /pubmed/34367729 http://dx.doi.org/10.1080/2162402X.2021.1945202 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
De Martin, Angelina
Lütge, Mechthild
Stanossek, Yves
Engetschwiler, Céline
Cupovic, Jovana
Brown, Kirsty
Demmer, Izadora
Broglie, Martina A.
Geuking, Markus B.
Jochum, Wolfram
McCoy, Kathy D.
Stoeckli, Sandro J.
Ludewig, Burkhard
Distinct microbial communities colonize tonsillar squamous cell carcinoma
title Distinct microbial communities colonize tonsillar squamous cell carcinoma
title_full Distinct microbial communities colonize tonsillar squamous cell carcinoma
title_fullStr Distinct microbial communities colonize tonsillar squamous cell carcinoma
title_full_unstemmed Distinct microbial communities colonize tonsillar squamous cell carcinoma
title_short Distinct microbial communities colonize tonsillar squamous cell carcinoma
title_sort distinct microbial communities colonize tonsillar squamous cell carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312615/
https://www.ncbi.nlm.nih.gov/pubmed/34367729
http://dx.doi.org/10.1080/2162402X.2021.1945202
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