Unified platform for genetic and serological detection of COVID-19 with single-molecule technology

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The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for...

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Autores principales: Furth, Noa, Shilo, Shay, Cohen, Niv, Erez, Nir, Fedyuk, Vadim, Schrager, Alexander M., Weinberger, Adina, Dror, Amiel A., Zigron, Asaf, Shehadeh, Mona, Sela, Eyal, Srouji, Samer, Amit, Sharon, Levy, Itzchak, Segal, Eran, Dahan, Rony, Jones, Dan, Douek, Daniel C., Shema, Efrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312974/
https://www.ncbi.nlm.nih.gov/pubmed/34310620
http://dx.doi.org/10.1371/journal.pone.0255096
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author Furth, Noa
Shilo, Shay
Cohen, Niv
Erez, Nir
Fedyuk, Vadim
Schrager, Alexander M.
Weinberger, Adina
Dror, Amiel A.
Zigron, Asaf
Shehadeh, Mona
Sela, Eyal
Srouji, Samer
Amit, Sharon
Levy, Itzchak
Segal, Eran
Dahan, Rony
Jones, Dan
Douek, Daniel C.
Shema, Efrat
author_facet Furth, Noa
Shilo, Shay
Cohen, Niv
Erez, Nir
Fedyuk, Vadim
Schrager, Alexander M.
Weinberger, Adina
Dror, Amiel A.
Zigron, Asaf
Shehadeh, Mona
Sela, Eyal
Srouji, Samer
Amit, Sharon
Levy, Itzchak
Segal, Eran
Dahan, Rony
Jones, Dan
Douek, Daniel C.
Shema, Efrat
author_sort Furth, Noa
collection PubMed
description The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients’ samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.
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spelling pubmed-83129742021-07-31 Unified platform for genetic and serological detection of COVID-19 with single-molecule technology Furth, Noa Shilo, Shay Cohen, Niv Erez, Nir Fedyuk, Vadim Schrager, Alexander M. Weinberger, Adina Dror, Amiel A. Zigron, Asaf Shehadeh, Mona Sela, Eyal Srouji, Samer Amit, Sharon Levy, Itzchak Segal, Eran Dahan, Rony Jones, Dan Douek, Daniel C. Shema, Efrat PLoS One Research Article The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients’ samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens. Public Library of Science 2021-07-26 /pmc/articles/PMC8312974/ /pubmed/34310620 http://dx.doi.org/10.1371/journal.pone.0255096 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Furth, Noa
Shilo, Shay
Cohen, Niv
Erez, Nir
Fedyuk, Vadim
Schrager, Alexander M.
Weinberger, Adina
Dror, Amiel A.
Zigron, Asaf
Shehadeh, Mona
Sela, Eyal
Srouji, Samer
Amit, Sharon
Levy, Itzchak
Segal, Eran
Dahan, Rony
Jones, Dan
Douek, Daniel C.
Shema, Efrat
Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_full Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_fullStr Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_full_unstemmed Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_short Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
title_sort unified platform for genetic and serological detection of covid-19 with single-molecule technology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312974/
https://www.ncbi.nlm.nih.gov/pubmed/34310620
http://dx.doi.org/10.1371/journal.pone.0255096
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