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Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a

Nicotiana tabacum is a non‐food herb that has the potential to be utilized as bio‐factory for generating medicines, vaccines or valuable small metabolites. To achieve these goals, the improvement of genetic tools for pre‐designed genome modifications is indispensable. The development of CRISPR/Cas n...

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Detalles Bibliográficos
Autores principales: Huang, Teng‐Kuei, Armstrong, Brittney, Schindele, Patrick, Puchta, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313123/
https://www.ncbi.nlm.nih.gov/pubmed/33511745
http://dx.doi.org/10.1111/pbi.13546