A multi‐functional genetic manipulation system and its use in high‐level expression of a β‐mannanase mutant with high specific activity in Pichia pastoris

To further extend the practical application of a thermostable and acidic resistance β‐mannanase (ManAK) in animal feed additives, an effective strategy that combined directed evolution and metabolic engineering was developed. Four positive mutants (P191M, P194E, S199G and S268Q) with enhanced specif...

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Detalles Bibliográficos
Autores principales: Liu, Zhemin, Cao, Linyuan, Fu, Xiaodan, Liang, Qingping, Sun, Han, Mou, Haijin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313266/
https://www.ncbi.nlm.nih.gov/pubmed/33942496
http://dx.doi.org/10.1111/1751-7915.13812

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