Effect of Yiqi Huoxue Granules on Platelet Activation Induced by Thrombin

OBJECTIVE: To study the effects of Yiqi Huoxue (YQHX) granules on platelet activation and aggregation induced by thrombin. METHODS: The effect of YQHX on platelet aggregation rate was detected by platelet aggregation instrument; the effect of YQHX on thrombosis time was observed by the mouse mesentery thrombosis model. DAMI cells were induced to transform into platelet-like granules using PMA, and the effects of SCH (PAR-1 inhibitor) on thrombin-induced changes in platelet intracellular calcium concentration, PAR-1 protein expression, and phosphorylation of MAPK were examined. RESULTS: Compared with the control group, the platelet aggregation rate, PAR-1 protein expression, phosphorylation of ERK1/2, and p38 protein in the YQHX group decreased (P < 0.05), and there was no significant difference between the YQHX + SCH group and YQHX group (P > 0.05). CONCLUSION: YQHX suppresses the platelet activation induced by thrombin by inhibiting PAR-1 expression.